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HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF SPERMACOCE HISPIDA.LINN AGAINST CARBON TETRACHLORIDE (CCl4) INDUCED HEPATOTOXICITY ON ALBINO WISTAR RATS.  [cached]
M.Karthikeyan
International Journal of Pharmaceutical Research and Development , 2011,
Abstract: In Indian traditional system of medicine, herbal remedies are prescribed for the treatment of various diseases including liver diseases. The present study was aimed to investigate the hapatoprotective activity of the Ethanolic Extract of Spermacoce hispida.Linn (SHE) against carbon tetra chloride (CCl4) inducd hepatotoxicity in rats. Liver functions were assessed by the determination of SGOT, SGPT, ALP and bilirubin. Histopathological studies were carried out.The serum biochemical analysis results suggest that the use of Ethanolic extract of Spermacoce hispida.Linn exhibited significant protective effect from hepatic damage in CCl4 induced hepatotoxicity model. Histopathological studies revealed that concurrent administration of the extract with CCl4 exhibited protective effect on the liver, which further evidenced its hepatoprotective activity.
Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats
Zim, M.C.A.;Silveira, T.R.;Schwartsmann, G.;Cerski, T.;Motta, A.;
Brazilian Journal of Medical and Biological Research , 2002, DOI: 10.1590/S0100-879X2002001100012
Abstract: few data are available in the literature regarding the effect of pentosan polysulfate (pps) on normal and fibrotic rat livers. in addition, the combination of pps and carbon tetrachloride (ccl4) has not been studied so far. the objective of this study was to assess the effect of pps on rat livers treated or not with ccl4 for the induction of liver fibrosis. the study consisted of four stages: 1) hepatic fibrosis induction with ccl4 (n = 36 rats); 2) evaluation of the effect of pps on ccl4-induced hepatic fibrosis (n = 36 rats); 3) evaluation of the effect of higher doses of pps in combination with ccl4 (n = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by pps (n = 18 rats) using the sodium pentobarbital test which indirectly evaluates hepatic microsomal enzyme activity in vivo. adult (60 to 70 days) male wistar rats weighing 180 to 220 g were used. all animals receiving 0.5 ml 8% ccl4 (n = 36) developed hepatic fibrosis, and after 8 weeks they also developed cirrhosis. no delay or prevention of hepatic fibrosis was observed with the administration of 5 mg/kg pps (n = 8) and 1 mg/kg pps (n = 8) 1 h after the administration of ccl4, but the increased hepatotoxicity resulting from the combination of the two substances caused massive hepatic necrosis in most rats (n = 45). pps (40 mg/kg) alone caused hepatic congestion only after 8 weeks, but massive hepatic necrosis was again observed in association with 0.5 ml ccl4 after 1 to 4 weeks of treatment. unexpectedly, sleeping time increased with time of pps administration (1, 2, or 3 weeks). this suggests that pps does not function as an activator of the hepatic microsomal enzymatic system. further studies are necessary in order to clarify the unexpected increase in hepatotoxicity caused by the combination of ccl4 and high doses of pps, which results in massive hepatic necrosis.
Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats  [cached]
Zim M.C.A.,Silveira T.R.,Schwartsmann G.,Cerski T.
Brazilian Journal of Medical and Biological Research , 2002,
Abstract: Few data are available in the literature regarding the effect of pentosan polysulfate (PPS) on normal and fibrotic rat livers. In addition, the combination of PPS and carbon tetrachloride (CCl4) has not been studied so far. The objective of this study was to assess the effect of PPS on rat livers treated or not with CCl4 for the induction of liver fibrosis. The study consisted of four stages: 1) hepatic fibrosis induction with CCl4 (N = 36 rats); 2) evaluation of the effect of PPS on CCl4-induced hepatic fibrosis (N = 36 rats); 3) evaluation of the effect of higher doses of PPS in combination with CCl4 (N = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by PPS (N = 18 rats) using the sodium pentobarbital test which indirectly evaluates hepatic microsomal enzyme activity in vivo. Adult (60 to 70 days) male Wistar rats weighing 180 to 220 g were used. All animals receiving 0.5 ml 8% CCl4 (N = 36) developed hepatic fibrosis, and after 8 weeks they also developed cirrhosis. No delay or prevention of hepatic fibrosis was observed with the administration of 5 mg/kg PPS (N = 8) and 1 mg/kg PPS (N = 8) 1 h after the administration of CCl4, but the increased hepatotoxicity resulting from the combination of the two substances caused massive hepatic necrosis in most rats (N = 45). PPS (40 mg/kg) alone caused hepatic congestion only after 8 weeks, but massive hepatic necrosis was again observed in association with 0.5 ml CCl4 after 1 to 4 weeks of treatment. Unexpectedly, sleeping time increased with time of PPS administration (1, 2, or 3 weeks). This suggests that PPS does not function as an activator of the hepatic microsomal enzymatic system. Further studies are necessary in order to clarify the unexpected increase in hepatotoxicity caused by the combination of CCl4 and high doses of PPS, which results in massive hepatic necrosis.
HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ALTHAEA OFFICINALIS LINN AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY ON ALBINO WISTAR RATS
Jabbar Zoobi,Ali Mohd
International Research Journal of Pharmacy , 2011,
Abstract: In Indian traditional system of medicine, herbal remedies are prescribed for the treatment of various diseases including liver diseases. The present study was aimed to investigate the hepatoprotective activity of the ethanolic extract of Althaea officinalis against Carbon tetrachloride induced hepatotoxicity in rats. Liver function were assessed by the determination of SGPT and SGOT studies. The serum biochemical analysis results suggested that the use of ethanolic extract of Althaea officinalis exhibited significant protective effect from hepatic damage in CCl4 induced hepatotoxicity model.
Hepatoprotective Activity of Ethanolic Extract of the Stems of Anisochilus Carnosus against Carbon Tetrachloride-induced Hepatotoxicity in Rats
P Venkatesh, A Dinakar, N Senthilkumar
International Journal of Health Research , 2010,
Abstract: Purpose: To evaluate the hepatoprotective activity of ethanolic extract of the stems of Anisochilus Carnosus (EEAC) against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Methods: Hepatotoxicity was induced in albino Wistar rats of either sex by intraperitoneal injection of CCl4 in olive oil (1:1). Two doses of ethanolic extract of Anisochilus Carnosus (200 and 400 mg/kg body weight) were administered to the experimental rats. The hepatoprotective effect of the extract was evaluated by the assay of liver function biochemical parameters like serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, alkaline phosphatase, total bilirubin and total protein. Results: In ethanolic extract treated animals, the toxic effect of CCl4 was significantly controlled by the plant extract as compared to the normal and the standard drug silymarin treated group. Conclusion: Ethanolic extract of stems of Anisochilus Carnosus possesses significant hepatoprotective activity.
Hepatoprotective Activity of Livobond A Polyherbal Formulation Against CCl4 Induced Hepatotoxicity in Rats  [PDF]
Usha S. Satyapal,Vilasrao J. Kadam,Rumi Ghosh
International Journal of Pharmacology , 2008,
Abstract: In the present study, Livobond was evaluated for its hepatoprotective effects against carbon tetrachloride-induced hepatocellular injury in rats. Hepatotoxicity was induced in male Sprague-Dawley rats by intraperitoneal injection of CCl4 (1.5 mL kg-1) in olive oil (1:1). Livobond at a dose of 500 and 750 mg/kg/day and silymarin standard 50 mg/kg/day was administered orally for 7 days. The hepatoprotective effect of Livobond and standard was evaluated by the assay of biochemical parameters viz., alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), total and direct bilirubin, liver lipid peroxidation, total proteins, catalase and by histopathological studies of the liver. The toxic effects of CCl4 in Livobond treated group was controlled significantly by restoration of the levels of serum bilirubin, proteins and enzymes as compared to the CCl4 treated and silymarin treated groups. Histopathological studies further confirmed the hepatoprotective activity of Livobond. The results suggest that Livobond is able to significantly alleviate the hepatotoxicity induced by CCL4 and may be attributed to the antioxidant property of the formulation.
Hepatoprotective Effect of the Aqueous Root-Bark Extract of Ficus sycomorus (Linn) on Carbon Tetrachloride Induced Hepatotoxicity in Rats
S.H. Garba,J. Prasad,U.K. Sandabe
Journal of Biological Sciences , 2007,
Abstract: The aqueous root-bark extract of Ficus sycomorus (Linn) was tested for its chemical constituents, acute toxicity and hepatoprotective effect against Carbon tetrachloride (CCl4) induced hepatotoxicity in rats. A total of 78 adult albino rats weighing between 150-320 g were used. The animals were each weighed at the start of the experiment and divided into two segments consisting of 42 rats for the acute toxicity and 36 rats for the hepatoprotective study segments, respectively. In the acute toxicity study the aqueous extract of the root-bark of Ficus sycomorus was administered intraperitoneally (ip) in a dose range of 0.2-12 g kg-1 and the rats were observed for the physical signs of toxicity for 24 h. The hepatoprotective segment involved dosing the negative control rats intraperitonealy with carbon tetrachloride (CCl4) 3 mL kg-1 that was dissolved in corn oil to induce liver damage while the treatments groups were pretreated with 640 mg kg-1 of the extract orally an hour before CCl4 (3 mL kg-1) was administered to observe if the extract has any hepatoprotective effect against CCl4 induced hepatotoxicity At the end of each treatment period, the animals were weighed and blood was obtained from animals administered CCl4 and pre-treated with 640 mg kg-1 of the extract for biochemical analysis with the liver extracted, weighed and processed for histological assessment. Phytochemical analysis of the extract revealed the presence of saponins, flavonoids, alkaloids, tannins and reducing sugar and LD50 was calculated as 3.20 0.6031 g kg-1. Pre-treatment of the rats with the extract was able to reduce though not significantly, changes in the biochemical parameters (decrease in albumin but increase in Aspartate Transaminase (AST), Alanine-Transaminase (ALT), Alkaline Phosphatase (ALP) and bilirubin) and preserved the liver parenchymal architecture against CCl4 induced degenerative changes, fibroplasia and cirrhosis. The results of this study showed that the plant extract had hepatoprotective effect on the parenchymal architecture of the liver against CCL4 induced hepatotoxicity in rats. But further studies to observe its hepatocurative potentials would be useful and is recommended.
Hepatoprotective effects of berberine on carbon tetrachloride-induced acute hepatotoxicity in rats
Yibin Feng, Ka-Yu Siu, Xingshen Ye, Ning Wang, Man-Fung Yuen, Chung-Hang Leung, Yao Tong, Seiichi Kobayashi
Chinese Medicine , 2010, DOI: 10.1186/1749-8546-5-33
Abstract: Sprague-Dawley rats aged seven weeks were injected intraperitoneally with 50% CCl4 in olive oil. Berberine was orally administered before or after CCl4 treatment in various groups. Twenty-four hours after CCl4 injection, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, serum and liver superoxide dismutase (SOD) activities were measured. Histological changes of liver were examined with microscopy.Serum ALT and AST activities significantly decreased in a dose-dependent manner in both pre-treatment and post-treatment groups with berberine. Berberine increased the SOD activity in liver. Histological examination showed lowered liver damage in berberine-treated groups.The present study demonstrates that berberine possesses hepatoprotective effects against CCl4-induced hepatotoxicity and that the effects are both preventive and curative. Berberine should have potential for developing a new drug to treat liver toxicity.Liver damage induced by carbon tetrachloride (CCl4) involves biotransformation of free radical derivatives, increased lipid peroxidation and excessive cell death in liver tissue [1,2]. This model of CCl4-induced liver injury has been widely used in new drug development for liver diseases.Berberine is a plant alkaloid present in many medicinal herbs, such as Hydrastis canadensis, Coptidis Rhizoma, Berberis aquifolium, Berberis aristata and Berberis vulgaris [3]. Coptidis Rhizoma (Huanglian), which is rich in berberine, exhibited hepatoprotective effects on CCl4-induced liver injury via scavenging the peroxidative products [4]. Antioxidative effects of Coptidis Rhizoma and its major active ingredient berberine against peroxynitrite-induced kidney damage were demonstrated in vitro and in vivo [5]. Previous studies reported that berberine inhibited inflammation [6] and low-density lipoprotein (LDL) oxidation [7]. Other studies found that berberine was a candidate drug for Alzheimer's disease [8] and cancer [9]. Berberine exhib
PROTECTIVE EFFECT OF Solanum Pubescens LINN ON CCL4 INDUCED HEPATOTOXICITY IN ALBINO RATS  [PDF]
M.Pushpalatha,T.Ananthi
Mintage Journal of Pharmaceutical and Medical Sciences , 2012,
Abstract: Ethanol extract of Solanum pubescens Linn was evaluated for hepato protective and antioxidant activities in rats. The plant extract (500mg/kg/day) showed a remarkable hepatoprotective and antioxidant activity against Carbon tetrachloride (CCl4)-induced hepatotoxicity as judged from the serum marker enzymes and antioxidant levels in liver tissues. CCl4 induced a significant rise in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, LPO with a reduction of total protein, superoxide dismutase (SOD), catalase, and reduced glutathione (GSH). Treatment of rats with plant extract (500 mg/kg) significantly (P<0.01) altered serum marker enzymes and antioxidant levels to near normal against CCl4 - treated rats. The activity of the extract at dose of 500 mg/kg was comparable to the standard drug, Silymarin (50 mg/kg, p.o.). Histopathological examination of the liver tissues supported the hepatoprotective activity of plant.
Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats
AB Saba, AA Oyagbemi, OI Azeez
Nigerian Journal of Physiological Sciences , 2010,
Abstract: This study was conducted to explore possible protective effect of Cnidoscolus aconitifolius (CA) leaf extract on carbon tetrachloride (CCl4)-induced hepatotoxicity and haemotoxicity in experimental animal models. Thirty six rats of six per group were used in this study. Group I received 10ml/kg normal saline as control. Group II-VI rats were administered with 1.25ml/kg body weight (bwt) of carbon tetrachloride intraperitonealy. Animals in groups III, IV, V and VI were however pre-treated with aqueous extract of Cnidoscolus aconitifolius at 100, 250, 500 and 750mg/kg body weight (bwt) respectively. Administration of CCL4 in untreated rats led to microcytic hypochromic anaemia, thrombocytopenia, increased erythrocyte fragility and stress induced leucocytosis accompanied with significant (P<0.05) increase in neutrophils and decrease (P<0.01) in lymphocyte counts. CCl4 also led to significant (P<0.05) increase in serum transaminases (ALT and AST) and phosphatase (ALP) respectively compared with control animals. Also, CCL4 produced significant (P<0.05) increase in serum blood urea nitrogen (BUN) and creatinine compared with normal rats. Pre-treatment with Cnidoscolus aconitifolius leaf extract brought about significant restoration of the haematological parameters to values that were comparable to those of the control with concomitant decrease (P<0.05) in the activities of the marker of hepatic damage enzymes (ALT, AST and ALP), in a dose-dependent manner. Similarly, serum levels of blood urea nitrogen (BUN) and creatinine were also brought to near normal by the CA in a dose-dependent manner. From this study, we conclude that pre-exposure to Cnidoscolus aconitifolius leaf extract considerably reduced the effect of CCl4 on the blood parameters and ameliorated hepatic damage by the haloalkane
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