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Isolation of Pasteurella multocida subspec. Multocida from chronic periapical lesion: First isolation in ex-Yugoslavia)  [PDF]
Suvajd?i? Ljiljana D.,Mr?a Ekaterina A.,D?ambas Ljubomir D.,Bogavac Mirjana A.
Zbornik Matice Srpske za Prirodne Nauke , 2006, DOI: 10.2298/zmspn0611029s
Abstract: This study presents five isolates of Pasteurella multocida subsp. multo-cida isolated from chronic periapical inflammatory lesion. We described the methods of sampling and cultivation as well as diagnostic criteria. Pasteurella multocida was diagnosed on the basis of characteristic cultural and tinctorial properties and the facts that all strains produced indole and induced ornithine decarboxilation, glucose, saccharose and manitole fermentation. Isolates produced neither urease, nor fermented lactose and maltose. Further classification to subspecies multocida was based on the fact that all investigated isolates fermented trechalose, xylose and sorbitol the traits which are diagnostically significant for the species. Patients deny any contact with farm animals or pets, which indicates a possible aerosol transport and animal-human as well as human-human infection. We consider that this organism should be paid more attention by dentist, oral surgeons and microbiologists.
Phenotypic variability among strains of Pasteurella multocida isolated from avian, bovine, caprine, leporine and ovine origin
SO Ekundayo, MO Odugbo, AO Olabode, PA Okewole
African Journal of Biotechnology , 2008,
Abstract: Phenotypic diversity among 69 field isolates plus 3 vaccine strains previously identified as Pasteurella multocida were investigated by extended phenotypic characterization. The field isolates were obtained in Nigeria from chickens (15 isolates), quail (5 isolates), cattle (31 isolates), goats (7 isolates), sheep (8 isolates), rabbits (3 isolates) and the vaccine strains (3 isolates), which are used as prophylaxis against fowl cholera and haemorrhagic septicaemia diseases. Consistent results were obtained for all isolates in the test for Gram reaction, oxidase, catalase, urease, no growth on MacConkey agar and nitrate reduction. All isolates also fermented D-glucose, D-mannitol, and sucrose but failed to ferment lactose. The isolates differed in their ability to ferment L-arabinose, D-dulcitol, D-sorbitol, D-xylose and in the production of indole and H2S in triple sugar iron agar resulting in the identification of 8 biochemical types or biovars. Dulcitol and sorbitol fermentation patterns meant that the isolates (including the vaccine strains) could be identified as subspecies Pasteurella multocida multocida (74%), Pasteurella multocida septica (18%), or Pasteurella multocida gallicida (8%). The subspecies P. m. multocida was demonstrated in all the animal species and the vaccine strains. Among the animal species studied, P.m. septica was demonstrated in all but the leporine species while P.m. gallicida was demonstrated only in the avian and ovine species. This characterization study adds to the considerable phenotypic variability that has been reported within the P. multocida taxon.
Meningitis bacteriémica por Pasteurella multocida Pasteurella multocida bacteremic meningitis  [cached]
R. Soloaga,N. Carrión,J. Pidone,M. Suar
Revista argentina de microbiolog?-a , 2008,
Abstract: Las infecciones por Pasteurella multocida en seres humanos habitualmente están relacionadas con mordeduras o ara azos de perros y gatos. Muchas de ellas se acompa an de otros microorganismos de la orofaringe de estos animales. Se presenta un caso de meningitis bacteriémica por P. multocida en una mujer de 86 a os que convivía con siete gatos. Si bien no se documentó una infección de piel o de partes blandas, es posible que ésta haya pasado inadvertida inicialmente y que fuera la causa de la bacteriemia con impacto en meninges, o bien que la meningitis se haya producido luego de la colonización nasofaríngea (no demostrada). Los aislamientos de hemocultivos y de líquido cefalorraquídeo fueron identificados como P. multocida por medio de API 20NE, API 20E y Vitek 1. La cepa aislada presentó sensibilidad a penicilina, cefotaxima, levofloxacina y tetraciclinas, en coincidencia con lo descrito en la literatura. Human infections by Pasteurella multocida are usually associated with bites or scratches from dogs and cats. Many of them are accompanied by other oropharyngeal microorganisms of these animals. We herein present a case of bacteremic meningitis by P. multocida in an 86-year-old woman who was living with seven cats. Even though no skin or soft tissue infection was recorded, it is possible that a mild infection had gone undetected and a subsequent bacteremia had impacted on the meninges, or that meningitis could have occurred after nasopharyngeal colonization (not demonstrated). The isolates obtained from blood cultures and cerebrospinal fluid were identified as P. multocida by API 20NE, API 20E, and Vitek 1. In agreement with findings in the literature, this strain was susceptible to penicillin, cefotaxime, levofloxacin and tetracyclines.
Identification of novel immunogens in Pasteurella multocida
Keith Al-Hasani, John Boyce, Victoria P McCarl, Stephen Bottomley, Ian Wilkie, Ben Adler
Microbial Cell Factories , 2007, DOI: 10.1186/1475-2859-6-3
Abstract: Bioinformatics analysis of the P. multocida genome predicted 129 proteins as secreted, located in the outer membrane, or lipoproteins. 105 of the genes encoding these proteins were cloned and recombinant protein expressed in Escherichia coli. Polyclonal serum from P. multocida-infected chickens reacted with a subset of these proteins.These data show the range of bacterial immunogens recognized by the chicken immune system, including 6 novel immunoreactive proteins.Pasteurella multocida is a Gram-negative bacterial pathogen which is the causative agent of a range of diseases in animals, including fowl cholera in avian species, hemorrhagic septicemia in ungulates, shipping fever and pneumonia in cattle, atrophic rhinitis in swine, and snuffles in rabbits [1-3]. The bacterium also causes infection in humans, primarily through dog and cat bites. Fowl cholera, which is generally caused by serotypes A:1, A:3 or A:4 [4], is a severe systemic disease which occurs in domestic poultry and wild birds and results in significant economic losses to poultry industries worldwide. Current vaccines against fowl cholera include bacterins [5], which provide only limited protection against homologous serotypes and live attenuated strains, which have been observed to revert to virulence [6]. Therefore, there is a need for more effective vaccines to control diseases caused by P. multocida.The surface of Gram-negative bacteria is critical for interaction of the bacterium with the host cell environment as it mediates nutrient uptake, secretion of toxins and other products and is involved in avoidance of the host immune system [7]. Furthermore, it is the bacterial surface molecules that are the targets for host immunity. Indeed, bacterial surface proteins have been shown to be important for conferring protective immunity in a range of infection models [8,9]. Recently, the P. multocida PlpB protein was identified as a cross-protective antigen [10,11] and this protein is located in the P. mult
Signaling Cascades of Pasteurella multocida Toxin in Immune Evasion  [PDF]
Katharina F. Kubatzky,Bianca Kloos,Dagmar Hildebrand
Toxins , 2013, DOI: 10.3390/toxins5091664
Abstract: Pasteurella multocida toxin (PMT) is a protein toxin found in toxigenic strains of Pasteurella multocida. PMT is the causative agent for atrophic rhinitis in pigs, a disease characterized by loss of nasal turbinate bones due to an inhibition of osteoblast function and an increase in osteoclast activity and numbers. Apart from this, PMT acts as a strong mitogen, protects from apoptosis and has an impact on the differentiation and function of immune cells. Many signaling pathways have been elucidated, however, the effect of these signaling cascades as a means to subvert the host’s immune system are just beginning to unravel.
Meningitis bacteriémica por Pasteurella multocida
Soloaga,R.; Carrión,N.; Pidone,J.; Suar,M.; Salinas,A.; Guelfand,L.; Alvarez,V.; Margari,A.; Cococcella,D.;
Revista argentina de microbiolog?-a , 2008,
Abstract: human infections by pasteurella multocida are usually associated with bites or scratches from dogs and cats. many of them are accompanied by other oropharyngeal microorganisms of these animals. we herein present a case of bacteremic meningitis by p. multocida in an 86-year-old woman who was living with seven cats. even though no skin or soft tissue infection was recorded, it is possible that a mild infection had gone undetected and a subsequent bacteremia had impacted on the meninges, or that meningitis could have occurred after nasopharyngeal colonization (not demonstrated). the isolates obtained from blood cultures and cerebrospinal fluid were identified as p. multocida by api 20ne, api 20e, and vitek 1. in agreement with findings in the literature, this strain was susceptible to penicillin, cefotaxime, levofloxacin and tetracyclines.
Isolation of pasteurella multocida from cows and buffaloes in Urmia's Slaughter House  [cached]
Karimkhani, H.,Zahraie Salehi, T.,Sadeghi Zali, M.H.,Karimkhani, M.
Archives of Razi Institute , 2011,
Abstract: Pasteurellosis is one of the important economic diseases in ruminants, especially in cows and buffaloes. It is caused by Pasteurella multocida and occasionally by Mannheimia haemolytica. The aim of this study was to isolate Pasteurella multocida from lungs with probable mentioned bacterial agents in cows and buffaloes in Urmia's slaughter house. 240 lung samples over a period of 12 months were cultured. The results have revealed 6 (2.5 %) Pasteurella multocida results suggest that the animal, its breed, sex, age and season can be effective in the occurrence of these positive cases. The positive samples were all from male beef cattle of hybrid breeds (4 samples) in winter and Holstein breeds (2 samples) in spring.
Pasteurella multocida Involved in Respiratory Disease of Wild Chimpanzees  [PDF]
Sophie K?ndgen, Michaela Leider, Felix Lankester, Astrid Bethe, Antina Lübke-Becker, Fabian H. Leendertz, Christa Ewers
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024236
Abstract: Pasteurella multocida can cause a variety of diseases in various species of mammals and birds throughout the world but nothing is known about its importance for wild great apes. In this study we isolated P. multocida from wild living, habituated chimpanzees from Ta? National Park, C?te d'Ivoire. Isolates originated from two chimpanzees that died during a respiratory disease outbreak in 2004 as well as from one individual that developed chronic air-sacculitis following this outbreak. Four isolates were subjected to a full phenotypic and molecular characterisation. Two different clones were identified using pulsed field gel electrophoresis. Multi Locus Sequence Typing (MLST) enabled the identification of previous unknown alleles and two new sequence types, ST68 and ST69, were assigned. Phylogenetic analysis of the superoxide dismutase (sodA) gene and concatenated sequences from seven MLST-housekeeping genes showed close clustering within known P. multocida isolated from various hosts and geographic locations. Due to the clinical relevance of the strains described here, these results make an important contribution to our knowledge of pathogens involved in lethal disease outbreaks among endangered great apes.
Pakistan Veterinary Journal , 2007,
Abstract: Outer membrane proteins (OMPs) play a significant role in the pathogenesis of Pasteurellosis. OMPs of Pasteurella multocida (P. multocida) B:2 strain were characterized by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Molecular weights of OMPs were determined by plotting graph between Rf value and log of molecular weight. A total of 6 polypeptides ranging from 15 kDa to 91 kDa were observed which included two intense bands of 39 and 32 kDa, and four less intense bands of 91, 72, 44 and 15 kDa. OMPs, therefore, can be used to identify strain specific markers that would form a useful basis to differentiate them from field isolates of P. multocida of same serotype i.e., B:2. However, this was a preliminary type of work and further research using various field isolates as well as vaccinal strains are needed.
Effects of Dexamethasone on Rats Infected with Pasteurella multocida
A.A. Mahmood,K. Sidik,I. Salmah,K.A.R. Suzainur
Journal of Animal and Veterinary Advances , 2012,
Abstract: Two groups of Sprague-Dawley adult male rats each consist of 8 animals were used throughout the experiment. All rats were subcutaneous inoculated once with 108cfu/ml of Pasteurella multocida strain PMB 202. In addition, Group 1 animals also received intra-muscular injection of dexamethasone (1.6 mg/animal/day) once daily for 9 days. Blood was collected from all animals before and after inoculation once daily for 9 days, for total and differential leucocytes count. Rats were scarified at day 9 for swabs culture and histological sections of liver, spleen and lung. In dexamethasone-treated animals (Group 1), total leucocytes count decreased after 24 hours (day 1) post-infection and then leucocytosis at day 4 (96 h), then leucopenia at day 8 and 9. Differential leucocytes count revealed gradual neutrophilia and lymphopenia at 120 h post infection (maximum), then neutropenia and lymphocytosis at day 7 and 9. Swab cultured from liver, spleen, and lung revealed positive for P. multocida and Staphylococcus aureus. Histological sections of such organs showed abscess formation surrounded by neutrophils. In Pasteurella multocida inoculated animals (Group 2), total leucocytes count started to increase gradually at day 1 and 2, then decreased to normal level at day 3 and leucocytosis at day 4 (maximum) and then gradual decreased to reach the normal level at day 9. Differential leucocytes count revealed, neutropenia and lymphocytosis at 24 hour post infection, and remain with the same level reaching maximun at day 9 with atypical lymphocytes. Swab cultured from liver, spleen and lungs showed negative for bacterial isolation. Histological sections of such organs reveled erythrocyte infiltration and mild infiltration of inflammatory cells.
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