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Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn  [PDF]
Pedro Mena,Luca Calani,Chiara Dall'Asta,Gianni Galaverna,Cristina García-Viguera,Renato Bruni,Alan Crozier,Daniele Del Rio
Molecules , 2012, DOI: 10.3390/molecules171214821
Abstract: The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.
Antinociceptive Effects of Pomegranate (Punica granatum L.) Juice and Seed Extracts on Acute Corneal Pain in Mice  [PDF]
Zahra Malek, Shirin Mansoori Dara, Mahsa Hadipour Jahromy
World Journal of Neuroscience (WJNS) , 2014, DOI: 10.4236/wjns.2014.42012
Abstract: In the present study, the antinociceptive effects of acute (2, 4 and 6 ml/kg) and chronic (1, 2 and 3 ml/kg for 14 days)oral administration ofpomegranate (Punica granatumL.) juice and seedextract with or without morphine and naloxane were investigatedonhypertonic saline-induced acute corneal pain perception in mice. The number of eye wipes with a forelimb was counted for a period of 30 seconds as the criterion for pain assessment. Acute oral administration of the extract(at 6 ml/kg dose, once) and chronic oral administration(at 2 and 3 ml/kg for 14 days each) significantly decreased the number of eye wipes after subcutaneous injection of morphine (2 mg/kg, sc), naloxone (2 mg/kg, sc) and normal saline (2 mg/kg, sc) compared with control (p < 0.05). The morphine-induced antinociception was significantly improved by both acute and chronic oral administrations of pomegranate extract (p < 0.05). Naloxone (2 mg/kg, sc) did not reverse the antinociceptive effects of acute (at 6 ml/kg dose, once, oral) and chronic (at 2 and 3 ml/kg for 14 days each) treatments. These findings demonstrate that acute high-dose and long-term lower-dose of pomegranate juice and seed extract can decrease acute corneal pain and improve morphine-induced antinociception in mice.
Pharmacological Characterization and Beneficial Uses of Punica granatum  [PDF]
Nakuleshwar Dut Jasuja,Richa Saxena,Subhash Chandra,Richa Sharma
Asian Journal of Plant Sciences , 2012,
Abstract: Punica granatum L. commonly known as Pomegranate belongs to the Family Punicaceae. Pomegranate has been known to be a reservoir of secondary metabolites which are being exploited as source of bioactive substance for various pharmacological purposes. Many researchers have focused their interest to investigate the bioactive compounds of Punica granatum for human health. In order to facilitate the further investigation and exploit the said plant, this study summarized herein the research achievements on some of the phytochemical and pharmacological properties of Punica granatum. The different types of phytochemicals have been identified from various parts of the pomegranate tree and from pomegranate juice, leaves, flower, fruits and seeds. The constituents of this plant were thoroughly reviewed discussed based on literatures.
The Effect of Two Methods of Pomegranate (Punica granatum L) Juice Extraction on Quality During Storage at C
Gra a Miguel,Susana Dandlen,Dulce Antunes,Alcinda Neves,Denise Martins
Journal of Biomedicine and Biotechnology , 2004, DOI: 10.1155/s1110724304403064
Abstract: The effect of two extraction methods of pomegranate juice on its quality and stability was evaluated. The first method consisted of separation of the seeds from fruits and centrifugation. The second method consisted of squeezing fruit halves with an electric lemon squeezer. During a period of 72 hours of cold storage at 4∘C, the juices were evaluated for the presence of sugars, organic acids, and anthocyanins. Delphinidin 3-glucoside was identified to be the major anthocyanin present at the level of 45–69 mg/L. Among the organic acids, oxalic and tartaric acids dominated. The major sugars detected in pomegranate juice were glucose and sucrose. No significant differences in the content of sugars, organic acids, or anthocyanins in juices obtained through application of the two different extraction methods were detected, with the exception of the drastic decrease of cyanidin 3,5-diglucoside level in juice obtained by seed centrifugation. The pH did not show differences between treatments. Titrable acidity and the level of sugars expressed as ∘Brix decreased after 32 and 15 hours after extraction, respectively, when juice was obtained by centrifuging the seeds.
The Effect of Two Methods of Pomegranate (Punica granatum L) Juice Extraction on Quality During Storage at 4 ° C  [cached]
Miguel Gra?a,Dandlen Susana,Antunes Dulce,Neves Alcinda
Journal of Biomedicine and Biotechnology , 2004,
Abstract: The effect of two extraction methods of pomegranate juice on its quality and stability was evaluated. The first method consisted of separation of the seeds from fruits and centrifugation. The second method consisted of squeezing fruit halves with an electric lemon squeezer. During a period of 72 hours of cold storage at 4 ° C, the juices were evaluated for the presence of sugars, organic acids, and anthocyanins. Delphinidin 3-glucoside was identified to be the major anthocyanin present at the level of 45–69 mg/L. Among the organic acids, oxalic and tartaric acids dominated. The major sugars detected in pomegranate juice were glucose and sucrose. No significant differences in the content of sugars, organic acids, or anthocyanins in juices obtained through application of the two different extraction methods were detected, with the exception of the drastic decrease of cyanidin 3,5 -diglucoside level in juice obtained by seed centrifugation. The pH did not show differences between treatments. Titrable acidity and the level of sugars expressed as ° Brix decreased after 32 and 15 hours after extraction, respectively, when juice was obtained by centrifuging the seeds.
Punica granatum (Pomegranate) juice provides an HIV-1 entry inhibitor and candidate topical microbicide
A Robert Neurath, Nathan Strick, Yun-Yao Li, Asim K Debnath
BMC Infectious Diseases , 2004, DOI: 10.1186/1471-2334-4-41
Abstract: Fruit juices were screened for inhibitory activity against HIV-1 IIIB using CD4 and CXCR4 as cell receptors. The best juice was tested for inhibition of: (1) infection by HIV-1 BaL, utilizing CCR5 as the cellular coreceptor; and (2) binding of gp120 IIIB and gp120 BaL, respectively, to CXCR4 and CCR5. To remove most colored juice components, the adsorption of the effective ingredient(s) to dispersible excipients and other foods was investigated. A selected complex was assayed for inhibition of infection by primary HIV-1 isolates.HIV-1 entry inhibitors from pomegranate juice adsorb onto corn starch. The resulting complex blocks virus binding to CD4 and CXCR4/CCR5 and inhibits infection by primary virus clades A to G and group O.These results suggest the possibility of producing an anti-HIV-1 microbicide from inexpensive, widely available sources, whose safety has been established throughout centuries, provided that its quality is adequately standardized and monitored.The global AIDS epidemic has proceeded relentlessly for ≈ 24 years with no promising prophylactic intervention in sight. In 2003 there were 5 million new HIV infections, and 3 million AIDS deaths [1]. To date the number of individuals living with HIV-1 infection/AIDS has reached 40 million, and ≈ 30 million people have already died from AIDS since the beginning of the pandemic [1,2]. Most new infections have been acquired by the mucosal route, heterosexual transmission playing the major (≈ 80%) role. Although the incidence of transmission per unprotected coital act is estimated to be low (0.0001 – 0.004), but strikingly increased when acutely infected individuals are involved [3,4], the cumulative effect is overwhelming.Anti-HIV-1 vaccines applicable to global immunization programs are not expected to become available for many years. Thus, other prevention strategies are urgently needed. This includes educational efforts and application of mechanical and/or chemical barrier methods. The latter correspond
Effectiveness of Apricots (Prunus armeniaca), Pomegranate (Punica granatum) Juice and Lactic Acid Fermented Sobya on Plasma Levels of Lipid Profile Parameters and Total Homocysteine among Egyptian Adults  [PDF]
Laila Hussein, Emam A. Abdel-Rahim, Abd El-Moneim MR Afify, Aly Ezz El-Arab, Eid Labib
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.522236
Abstract: Objectives: The aim of this study was to investigate the effectiveness of dietary intervention with apricots, pomegranate juice or lactic acid fermented sobya on improving the plasma levels of lipid profile parameters and total plasma homocysteine (tHcy) among healthy adults. Design of the study: Thirty-five men and women (28.9 ± 3.0 years) with mean body mass index of 23.5 ± 1.2 kg/m2 were randomly assigned to a dietary intervention study. During the pre-feeding study records of 24-hour food intake, anthropometric measurements and blood sampling were collected for biochemical investigations. Thereafter the volunteers were divided into five groups: a control group (C); the other four groups received daily one of the following supplements: 200 g of deep yellow apricots (Prunus armeniaca) (AF); 250 g pomegranate (Punica granatum) juice (PJ); mixture of 100 g (PJ) and 150 g of fermented sobya (FS), (PJ-FS) or 165 g (FS). After completing the three-week dietary intervention, blood samples were collected and blood indices were again evaluated. Biochemical analysis of total lipids (TL), total glycerides (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) and tHcy were completed. The low density lipoprotein-cholesterol level and three indices of risk factors were calculated using the appropriate equations. Results: Three-week dietary intervention improved the levels of lipid profile parameters TG, TC, HDL-C and LDL-C as compared to the respective baseline levels and the response of the control group that did not receive any supplement. Consuming (PJ-FS) had a synergistic beneficially added value on some of the above mentioned lipid profile parameters. The mean plasma tHcy concentrations; a marker for occlusive vascular disease was reduced significantly following the three week consumption of (PJ) or (FS), compared with the respective pre-feeding levels. Conclusion: Pomegranate juice (PJ) or lactic acid fermented sobya (FS) or mixture of both are potential natural agents in lowering the plasma levels of lipid profile parameters and tHcy among apparently healthy young women and men, without dyslipidemic symptoms.
Antimicrobial Ellagitannin of Punica granatum Fruits
Machado, Thelma de B.;Leal, Ivana C. R.;Amaral, Ana Claudia F.;Santos, Kátia R. N. dos;Silva, Marlei G. da;Kuster, Ricardo M.;
Journal of the Brazilian Chemical Society , 2002, DOI: 10.1590/S0103-50532002000500010
Abstract: the ethyl acetate extract of punica granatum fruits was fractionated by chromatographic techniques to afford the ellagitannin punicalagin. the substance was found to be active against methicillin-resistant staphylococcus aureus strains and was identified by hplc/uv and 1hnmr. the antibacterial assays which guided the isolation of the tannin were conducted using the disc diffusion method. minimum inhibitory concentration (mic) was determined by the dilution method according to nccls (national committee for clinical laboratory standards) procedure.
Antimicrobial Ellagitannin of Punica granatum Fruits  [cached]
Machado Thelma de B.,Leal Ivana C. R.,Amaral Ana Claudia F.,Santos Kátia R. N. dos
Journal of the Brazilian Chemical Society , 2002,
Abstract: The ethyl acetate extract of Punica granatum fruits was fractionated by chromatographic techniques to afford the ellagitannin punicalagin. The substance was found to be active against methicillin-resistant Staphylococcus aureus strains and was identified by HPLC/UV and 1HNMR. The antibacterial assays which guided the isolation of the tannin were conducted using the disc diffusion method. Minimum inhibitory concentration (MIC) was determined by the dilution method according to NCCLS (National Committee for Clinical Laboratory Standards) procedure.
DETERMINATION OF ANTIBACTERIAL ACTIVITY OF PUNICA GRANATUM FRUIT
P. P. Lasure*, N. M. Munot and S. S. Lawande
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: Punica granatum L. belongs to the family Punicaceae, which is originating from the Middle East, extending throughout the Mediterranean, eastward to China and India. Punica granatum has been used extensively as a traditional medicine in many countries for the treatment of dysentery, diarrhea, helminthiasis, acidosis, hemorrhage and respiratory pathologies. In addition, P. granatum is reported to have antioxidant, anti-atherosclerotic and antiviral properties. The dried pomegranate peels (20gms) were extracted using water (100ml) & ethanol (100 ml). The Ethanolic Extract of Punica granatum (EPG) was active against the tested bacteria. EPG demonstrated promising antibacterial activity against tested gram positive and gram negative bacteria. The E. coli and S. aureus shows more susceptibility to EPG.
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