We report here the application of a medicinally
important plant Amaranthus spinosus for the synthesis of gold nanoparticles (AuNPs). Different concentrations of
ethanolic leaf extract of the plant were reacted with aqueous solution of HAuCl4·4H2O
under mild reaction conditions. Synthesis of AuNPs was confirmed from the
UV-Vis study of surface plasmon resonance property of the colloidal solution.
Transmission electron microscopy (TEM) revealed particles as spherical and
triangular in shape. X-ray diffraction (XRD) confirmed the crystalline nature
of AuNPs with average size of 10.74 nm as determined by Debye-Scherrer’s Equation.
Fourier transform infra-red (FT-IR) analysis of leaf extract and lyophilized
AuNPs showed the presence of various functional groups present in diverse
phytochemicals. Energy dispersive X-ray (EDX) of purified AuNPs confirmed the
formation of AuNPs and surface adsorption of biomolecules. We further
investigated the toxicity of the synthesized AuNPs and found non toxic to the
cancer cell lines and could be used for biomedical applications.