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In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control  [PDF]
Maria Helena Madruga Lima-Ribeiro, Ralph Santos-Oliveira, Mauricélia Firmino de Santana, Terezinha de Jesus Andreoli Pinto, Irene Satiko Kikuchi, Cheila Gon?alves Mothé, Luana Cassandra Breitenbach Barroso Coelho, Maria Tereza dos Santos Correia, Ana Maria dos Anjos Carneiro-Le?o
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2012, DOI: 10.4236/jcdsa.2012.22011
Abstract: Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60Co gamma rays in a dose of 7.549 kGy·h–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.
Megastimanes and ergostane type steroid from leaves Cratylia mollis (Leguminosae)
Lima, Luciano S.;Lima, Marcos V. B.;David, Juceni P.;Giulietti, Ana M.;Queiroz, Luciano P. de;David, Jorge M.;
Journal of the Brazilian Chemical Society , 2009, DOI: 10.1590/S0103-50532009001000022
Abstract: from the methanolic extract of the leaves of cratylia mollis were obtained by chromatographic techniques (3s,5s,6s,9r)-3,6-dihydroxy-5,6-dihydro- β-ionol (1), and a new bis-norisoprenoid named (4s*, 6s*)-4-but-1e-enyl-4,6-dihydroxy-3,5,5-trimethyl-cyclohex-2-enone (2) as well as 5a,8a-epidioxyergosta-6,22-dien-3-β-ol. the structures of the pure compounds were elucidated based on ms, 1h and 13c nmr spectroscopic data analyses.
Histochemical Evaluation of Human Prostatic Tissues with Cratylia mollis Seed Lectin
Amanda L. R. de Lima,Carmelita C. B. Cavalcanti,Mariana C. C. Silva,Patrícia M. G. Paiva,Luana C. B. B. Coelho,Eduardo I. C. Beltr o,Maria T. dos S. Correia
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/179817
Abstract: Lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation. Cratylia mollis seed lectins (Cramoll 1,4 and Cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (NP), hyperplasia (BPH), and prostate carcinoma (PCa). The staining pattern of Con-A and Cramoll 1,4 in BPH was more intense than in NP. These lectins also showed staining differences between BPH and PCa; the latter showing decreased staining intensity with an increased degree of malignancy. PNA and Cramoll 3 stained epithelial cells similarly in all diagnoses although they did present intense staining of PCa glands lumen. Corpora amylacea were not differentially recognized by any of the lectins. Cramoll 1,4 and Cramoll 3 seed lectins present themselves as candidates for histochemical probes for prostate pathologies when compared to commercial lectins such as Con-A and PNA.
Controles físico, físico-químico, químico e microbiológico dos frutos de Dimorphandra mollis Benth., Fabaceae
Hubinger, Silviane Z.;Salgado, Hérida R. N.;Moreira, Raquel R. D.;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000500007
Abstract: considering the quality of phytotherapic agents, it is important to point out that the concern with this subject includes rigorous attendance the different steps of development and production of these products, from the collection of the vegetable to the availability of the final product. the quality control for the plants and their extracts is essential when they are used in the development of phytotherapics. in this work, methodologies (pharmacopoeic and no pharmacopoeic) were employed to establish norms for the quality control of dimorphandra mollis benth. (leguminosae) fruit, popularly known as faveira. pharmacopoeic and no pharmacopoeic methodologies were employed to physic, physic-chemical, chemical and microbiological quality control. the results obtained established the physic-chemical characters of the drug and showed that the fruits and the ethanol 70% extract present a content of flavonoids of 10.25% and 17.21%, respectively. the preliminary phytochemical analysis of the d. mollis fruits evidenced tannins, saponnins and flavonoids. the microbiological analysis did not present pathogenic growth among the accomplished tests.
Evidence of an endogenous lectin receptor in seeds of the legume Cratylia floribunda
Ramos, Márcio Viana;Bomfim, Liezelotte Rezende;Bandeira,;Debray, Henri;
Brazilian Journal of Plant Physiology , 2002, DOI: 10.1590/S1677-04202002000300003
Abstract: cratylia floribunda seeds were ground and the clean crude saline extract was fractionated into albumin, globulin, prolamin, acidic and basic glutelin protein fractions. these protein fractions were examined for the presence of an endogenous lectin receptor by sds-polyacrylamide gel electrophoresis, western blot, affinity chromatography on a sepharose 4b-cratylia floribunda (cfl) lectin column and kinetic analysis in real time by surface plasmon resonance (spr). prolamin was the richest protein fraction although very poor in haemagglutinating activity. basic glutelin was far the less interesting fraction for lectin activity and protein content, even though this fraction contains considerable amounts of carbohydrates. lectin was present in all protein fractions as estimated by haemagglutinating assays but basic glutelins were almost devoid of lectin activity. except for prolamins, protein bands were detected by sds-page in all other fractions. western blot using digoxigenin labelled con a revealed a single band in the albumin, globulin, acidic and basic glutelin fractions, which specifically interacted with cona. this band migrated exactly at the same position in such fractions and seemed to be more important in the globulins. affinity chromatography of the protein fractions on a sepharose-cfl column yielded a peak, which was only recovered after elution with acidic buffered solution or with an a-d-mannose solution and the monosaccharide was recognized by the lectin. these results were fully corroborated by real time interaction of immobilized cfl with the different soluble protein fractions suggesting the presence of a lectin receptor within albumins, globulins and basic glutelins. as a whole, the results suggest that the lectin from cratylia floribunda recognizes a soluble endogenous glycosylated receptor through an interaction mediated by its carbohydrate-binding site.
Evidence of an endogenous lectin receptor in seeds of the legume Cratylia floribunda  [cached]
Ramos Márcio Viana,Bomfim Liezelotte Rezende,Bandeira,Debray Henri
Brazilian Journal of Plant Physiology , 2002,
Abstract: Cratylia floribunda seeds were ground and the clean crude saline extract was fractionated into albumin, globulin, prolamin, acidic and basic glutelin protein fractions. These protein fractions were examined for the presence of an endogenous lectin receptor by SDS-polyacrylamide gel electrophoresis, western blot, affinity chromatography on a Sepharose 4B-Cratylia floribunda (CFL) lectin column and kinetic analysis in real time by surface plasmon resonance (SPR). Prolamin was the richest protein fraction although very poor in haemagglutinating activity. Basic glutelin was far the less interesting fraction for lectin activity and protein content, even though this fraction contains considerable amounts of carbohydrates. Lectin was present in all protein fractions as estimated by haemagglutinating assays but basic glutelins were almost devoid of lectin activity. Except for prolamins, protein bands were detected by SDS-PAGE in all other fractions. Western blot using digoxigenin labelled Con A revealed a single band in the albumin, globulin, acidic and basic glutelin fractions, which specifically interacted with ConA. This band migrated exactly at the same position in such fractions and seemed to be more important in the globulins. Affinity chromatography of the protein fractions on a Sepharose-CFL column yielded a peak, which was only recovered after elution with acidic buffered solution or with an alpha-D-mannose solution and the monosaccharide was recognized by the lectin. These results were fully corroborated by real time interaction of immobilized CFL with the different soluble protein fractions suggesting the presence of a lectin receptor within albumins, globulins and basic glutelins. As a whole, the results suggest that the lectin from Cratylia floribunda recognizes a soluble endogenous glycosylated receptor through an interaction mediated by its carbohydrate-binding site.
Toxicity of Dimorphandra mollis to Workers of Apis mellifera
Cintra, Priscila;Malaspina, Osmar;Petacci, Fernando;Fernandes, Jo?o B.;Bueno, Odair C.;Vieira, Paulo C.;Silva, M. Fátima das G. F. da;
Journal of the Brazilian Chemical Society , 2002, DOI: 10.1590/S0103-50532002000100019
Abstract: in this communication we have evaluated the toxic properties of methanol extracts from flowers, peduncles, leaves, petioles and stem bark of dimorphandra mollis to apis mellifera workers. astilbin (5,7,3',4'-tetrahydroxy?2,3-dihydroflavonol-3-b-o-rhamnoside) has been isolated from peduncles and flowers of this plant in large amounts. astilbin presented insecticidal activity against confined bees. the results suggest that astilbin reduces the average survival of treated bees.
Toxicity of Dimorphandra mollis to Workers of Apis mellifera  [cached]
Cintra Priscila,Malaspina Osmar,Petacci Fernando,Fernandes Jo?o B.
Journal of the Brazilian Chemical Society , 2002,
Abstract: In this communication we have evaluated the toxic properties of methanol extracts from flowers, peduncles, leaves, petioles and stem bark of Dimorphandra mollis to Apis mellifera workers. Astilbin (5,7,3',4'-tetrahydroxy--2,3-dihydroflavonol-3-beta-O-rhamnoside) has been isolated from peduncles and flowers of this plant in large amounts. Astilbin presented insecticidal activity against confined bees. The results suggest that astilbin reduces the average survival of treated bees.
Phytochemistry and phytotoxic activity of lagascea mollis (asteraceae)
Alarcón,S. R; Ocampos,L. S. N.; Flores Galleguillo,L. V.; Pacciaroni,A; Sosa,V. E.;
The Journal of Argentine Chemical Society , 2007,
Abstract: in the course of a phytochemical study of l. mollis cav. the previously reported compounds patuletin-7-o-glucoside 1, 12-hydroxy-13-en-xanthorrhizol 2, 11-en-13-hydroxy-xanthorrhizol 3, xanthorrhizol 4, 12,13-epoxy-xanthorrhizol 5, and 1-α-angeloyloxycarotol 6, were isolated. the etoh extract of the aerial parts showed selective phytotoxic effects on s.halepense. compounds 2 and 3 exhibited potent herbicidal activity on s. halepense. additionally, at all assayed doses, compound 2 affected drastically the root growth.
Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats
Feres, Cássia A. O.;Toledo, Vicente P. C. P.;Tagliati, Carlos A.;Piedade, Josiane B.;Hermont, Fernanda;Rocha, Orivaldo A. A.;Guimar?es, Tania M. P. D.;
Revista Brasileira de Farmacognosia , 2010, DOI: 10.1590/S0102-695X2010000400022
Abstract: dimorphandra mollis benth., fabaceae, also known as "faveira" or "fava-d'anta", is a plant common to the central woodsy meadow region of brazil. it is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. its principal component is rutin. the objective of this study is the evaluation of the safety of the use of the dried d. mollis extract in rodents. the rutin content of the standardized extract was 76.0±3%. with respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of d. mollis were observed, but an increase in eosinophiles occurred. hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of d. mollis. in the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in igg was only observed in the studies with a 2000 mg/kg dose. the results obtained with rodents suggest that no toxicity exists with the administration of dried d. mollis extract in a 1000 mg/kg dose.
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