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Vitellogenin levels in hemolymph, ovary and hepatopancreas of the freshwater crayfish Cherax quadricarinatus (Decapoda: Parastacidae) during the reproductive cycle
Lilian E. Ferré,Daniel A. Medesani,C. Fernando García,Matías Grodzielski
Revista de Biología Tropical , 2012,
Abstract: The freshwater crayfish Cherax quadricarinatus is a tropical species of great interest for aquaculture. Vitellogenin (Vg), a lipoprotein precursor of the vitellum accumulated in spawned eggs, can be synthesized in the ovary and/or hepatopancreas of most crustaceans, being the hemolymph the way for transporting Vg throughout the reproductive cycle. Concentration of Vg in hemolymph, ovary and hepatopancreas of Cherax quadricarinatus adult females was measured by means of ELISA, specifically developed after purifying the native Vg. Measurements were made at four periods of the reproductive cycle: pre-reproductive, mid-reproductive, late reproductive and post-reproductive. Besides, both hepatosomatic (HSI) and gonadosomatic (GSI) indexes were determined in each period. Significant variations in Vg levels were detected in both hemolymph and hepatopancreas, being the highest values observed during the mid-reproductive period. Besides, such variations were positively correlated to the HSI. A positive correlation between Vg levels in hepatopancreas and ovary was also seen. These results support previous evidences about the central role of the hepatopancreas as a site of Vg synthesis in the studied species, together with the relevancy of hemolymph for transporting Vg from the hepatopancreas to the ovary. For aquaculture purposes, Vg monitoring in hemolymph could be used as a non-injurious method, to check the reproductive activity of C. quadricarinatus females. La langosta de agua dulce Cherax quadricarinatus es una especie tropical de gran interés para la acuicultura. Se midió la concentración de vitelogenina (Vg) en hemolinfa, ovario y hepatopáncreas de hembras adultas de esta especie, por medio de ELISA. Las mediciones fueron hechas en los cuatro períodos del ciclo reproductivo: pre-reproductivo, reproductivo medio, reproductivo tardío y post-reproductivo. Se detectaron variaciones significativas en los niveles de Vg tanto en hemolinfa como en hepatopáncreas, se observó el mayor valor durante el período reproductivo medio. Además, tales variaciones se correlacionaron positivamente con el índice hepatosomático. Se observó además una correlación positiva de los niveles de Vg entre hepatopáncreas y ovario. Estos resultados apoyan evidencias previas sobre el papel central del hepatopáncreas como sitio de síntesis de Vg, en esta especie, y también enfatizan la importancia de la hemolinfa para el transporte de la Vg del hepatopáncreas al ovario. Para propósitos de acuicultura, la medición de Vg en hemolinfa podría ser utilizada como un método no lesivo, con el fin
Determination site of vitellogenin synthesis in freshwater crayfish Cherax quadricarinatus at different maturation stages females Determinación del lugar de síntesis de la vitelogenina en hembras de la langosta de agua dulce Cherax quadricarinatus en diferentes estados de maduración  [cached]
Vania Serrano-Pinto,María Guadalupe Carrisoza-Valenzuela,Martín Ramírez-Orozco
Investigaciones Marinas , 2005,
Abstract: . The objective of this study was to determine the site of vitellogenin (Vg) synthesis at different stages of female maturation of freshwater crayfish Cherax quadricarinatus. PCR products of 1,100 bp and 900 bp were generated from genomic DNA in the first case, and hepatopancreas and ovary cDNAs in the second case. Results from RT-PCR analyses showed that the mRNA encoding the 3' end of the Vg cDNA was present in the hepatopancreas from secondary-vitellogenesis at first maturation, previously spawner and ovigerous females. The Vg mRNA was present simultaneously in the ovary from secondary-vitellogenesis at first maturation only, but was not detected at previously spawning and ovigerous females. This study provided evidence that the ovary plays a significant role in the production of this major egg yolk protein, but only in some stage of the vitellogenesis cycle El objetivo de este estudio fue determinar el lugar de síntesis de la vitelogenina (Vg) en hembras de la langosta de agua dulce Cherax quadricarinatus en diferentes estados de madurez. Un producto de PCR de 1.100 y 900 pb fueron generados a partir de ADN genómico en el primer caso, y de ADNc de tejido de hepatopáncreas y de ovario, en el segundo caso. Resultados del análisis del RT-PCR se alaron que el ARNm que codifica el extremo 3' de ADNc de la Vg estuvo presente en hepatopáncreas de hembras en vitelogénesis secundaria de primera maduración, de hembras con desoves anteriores y de hembras ovígeras. El ARNm de la Vg estuvo presente simultáneamente en el ovario de hembras en vitelogénesis secundaria de primera maduración, pero no estuvo presente en el ovario de hembras con desoves anteriores y de hembras ovígeras. Este estudio da evidencia de que el ovario juega un papel importante en la producción de la principal proteína del vitelo, pero solamente en ciertas etapas del ciclo de la vitelogénesis
The effect of protein and energy levels in diet on the antioxidant activity of juvenile redclaw Cherax quadricarinatus (von Martens, 1868) Efecto del nivel de proteína y energía en la actividad antioxidante de juveniles de langosta de agua dulce Cherax quadricarinatus (von Martens, 1868)  [cached]
Edilmar Cortés-Jacinto,ángel I. Campa-Córdova,Felipe Ascencio,Humberto Villarreal-Colmenares
Hidrobiológica , 2009,
Abstract: This study determined the influence of dietary crude protein and energy levels on growth, survival, and antioxidant response of superoxide dismutase (SOD) in juvenile redclaw Cherax quadricarinatus. Six practical diets containing three crude protein (CP) levels (28, 35, and 40%) and two energy levels (~18 and 20 kJ · g-1) were tested. The control group was fed a commercial shrimp diet. The highest SOD activity (34.8 U·mg-1 protein) occurred in the diet with 35% CP and 18 kJ g-1. Soluble protein in tissue of redclaw fed 28 and 35% CP increased, compared to the control. The best mean final weight (9.23 g), weight gain (788%), and the lowest feed conversion ratio (1.3) occurred with the 35% CP diet. We conclude that a dietary CP content of 35% and 18 kJ · g-1 is recommended for production and best immune responses in redclaw. Se realizó un estudio para evaluar el efecto de los niveles de proteína y energía sobre el crecimiento, supervivencia y la respuesta antioxidante de la enzima superóxido dismutasa (SOD) en juveniles de Cherax quadricarinatus. El experimento incluyó seis dietas prácticas con tres niveles de proteína cruda (28, 35 y 40%) y dos niveles de energía (~18 y 20 kJ·g-1). El grupo control fue alimentado con alimento comercial para camarón. La actividad SOD más alta (34.8 U·mg-1 prot.) se presentó en juveniles alimentados con 35% de proteína cruda (PC) y 18 kJ·g-1. La proteína soluble en tejido de langosta de agua dulce alimentada con 28 y 35% de PC fue superior con respecto a los acociles del grupo control. El mejor peso final promedio (9.23 g), la mejor ganancia en peso (788%) y el factor de conversión alimenticia mas bajo (1.3) se presentó en acociles alimentados con 35% de PC. Se concluye que una dieta conteniendo 35% de PC y 18 kJg-1 de energía es recomendada para la producción y además incrementa la respuesta inmune de los acociles en su fase de juvenil.
Efecto de diversos sustratos artificiales en el crecimiento y supervivencia de estadios tempranos de la langosta azul (Cherax quadricarinatus) cultivados en un sistema de recirculación - Effect of different artificial substrates on growth and survival of early stages of the blue lobster (Cherax quadricarinatus) reared in a recirculating system  [PDF]
García-Ulloa Gómez, Manuel,Pinzón López, Liliana Araceli
REDVET , 2012,
Abstract: ResumenSe evaluó la influencia de diferentes sustratos artificiales (tubo, malla, tubo + malla y grupo control, sin sustrato) sobre el crecimiento y la supervivencia de crías tempranas de Cherax quadricarinatus, cultivadas en un sistema derecirculación durante cuatro semanas.AbstractThe effect of different artificial substrates (tube or straw, mesh, mesh and tube or straw, and control or no substrate) on growth and survival of offspring early Cherax quadricarinatus raised in a recirculating system, was evaluated during four months.
Treatments for Temnocephalids Ectosymbiont Craspedella sp. on Cherax quadricarinatus and Cherax albertisii “Papua Freshwater Lobster”  [PDF]
Desy Sugiani, Angela Mariana Lusiastuti, Taukhid ?, Uni Purwaningsih
World Journal of Engineering and Technology (WJET) , 2015, DOI: 10.4236/wjet.2015.34C006
Abstract:

There were two species of crayfish “red-claw” (Cherax quadricarinatus) and “blue-huna” (Cherax albertisii) for their aquaculture potential. Crayfish were susceptible to fungal (crawfish plague), parasitic (protozoa and nematodes), and bacterial pathogen. A number of ectosymbiont Craspedella sp. have been observed on red-claw and blue-huna. The flatworms were commonly found almost in the whole body, on the upper exoskeleton behind the head, in the gill cavity and on the claws and underside of crayfish. Although their number sometimes was very high, they didn’t cause any problems especially for the new molting crayfish. Micro organisms living on the crayfish surface body and worms didn’t cause any pathological changes. Adults Craspedella sp. can be eliminated by a short bath in salt water or formaldehyde 37% solutions for several hours. This treatment didn’t kill worm eggs, so it needs to be repeated every one week. Moreover, hyposalinity or OST (Osmotic Shock Therapy) is one of the most effective therapies for ectoparasites on Craspedella sp. with dose of bath treatment 15 grams per litre of salt (15 ppt) for more than 3 hours, and dipped in salt water at 30 ppt (or 3.0 ppm, seawater salinity) for 15 - 20 minutes.

Determination site of vitellogenin synthesis in freshwater crayfish Cherax quadricarinatus at different maturation stages females
Serrano-Pinto,Vania; Carrisoza-Valenzuela,María Guadalupe; Ramírez-Orozco,Martín;
Investigaciones marinas , 2005, DOI: 10.4067/S0717-71782005000200006
Abstract: . the objective of this study was to determine the site of vitellogenin (vg) synthesis at different stages of female maturation of freshwater crayfish cherax quadricarinatus. pcr products of 1,100 bp and 900 bp were generated from genomic dna in the first case, and hepatopancreas and ovary cdnas in the second case. results from rt-pcr analyses showed that the mrna encoding the 3' end of the vg cdna was present in the hepatopancreas from secondary-vitellogenesis at first maturation, previously spawner and ovigerous females. the vg mrna was present simultaneously in the ovary from secondary-vitellogenesis at first maturation only, but was not detected at previously spawning and ovigerous females. this study provided evidence that the ovary plays a significant role in the production of this major egg yolk protein, but only in some stage of the vitellogenesis cycle
Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus)  [PDF]
EDI PRIYONO,OKID PARAMA ASTIRIN,PRABANG SETYONO
Nusantara Bioscience , 2009,
Abstract: Priyono E, Astirin OP, Setyono P. 2009. Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus). Nusantara Bioscience 1: 123-130. This research denotes to know the influence of biochemic composition to the rapid grow of fresh water lobster on the stadium of post larva (PL) of 60 within three months. This research used the complete random planning dealing with 4 treatments and each treatment would get 3 times cycle. The treatments cover, group K tested animal was treted with 100% mill food containing 30% of protein. Group A is given with food and biochemic food containing 13,34% of protein. Group B is the treated with mill food which is mixed with biochemic food containing 10,7% of protein. While group C was tested by treating them with mill food and biochemic food containing 13,58%. After all the above mentioned would be set up within 3:1 comparation. The variable of this research were the length of the abdomen, cephalothorax, total length, and the wet weight. The data analysis is using ANOVA system on 95% power test completed by of SPSS version 13. The result of the research shows that mentioned treatments give us the same influence toward the growth of fresh water lobster. The composition of biochemic food with the containing protein around 10,7%,13.34% and 13,38% has given the same effect to the lobster growth on post larva 60 level. There is strong correlation between abdomen and cephalothorax and between the total length and the lobsters weight.Key words: Cherax quadricarinatus, suplementary food, water quality.Abstrak. Priyono E, Astirin OP, Setyono P. 2009. Alternatif penambahan suplemen hayati untuk meningkatkan pertumbuhan udang lobster air tawar (Cherax quadricarinatus). Nusantara Bioscience 1: 123-130. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian suplemen hayati sehingga dapat meningkatkan pertumbuhan lobster air tawar pada stadia post larva (PL) 60 pada masa pertumbuhan 3 bulan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 macam perlakuan, masing-masing dengan 3 kali ulangan. Perlakuan yang diberikan meliputi, kelompok K hewan uji diberikan pakan pabrik 100% dengan kadar protein 30%, kelompok A hewan uji diberikan pakan pabrik ditambah suplemen hayati dengan kadar protein 13,34%, kelompok B hewan uji diberikan pakan pabrik dicampur dengan suplemen hayati kadar protein 10,7%, sedangkan kelompok C hewan uji diberikan pakan pabrik ditambah suplemen hayati dengan kadar protein 13,58% masing-masing dengan perbandingan 3:1. Variabel yang diamati adalah panjang cep
The effect of protein and energy levels in diet on the antioxidant activity of juvenile redclaw Cherax quadricarinatus (von Martens, 1868)
Cortés-Jacinto, Edilmar;Campa-Córdova, ángel I.;Ascencio, Felipe;Villarreal-Colmenares, Humberto;Holguín-Pe?a, Ramón J.;
Hidrobiológica , 2009,
Abstract: this study determined the influence of dietary crude protein and energy levels on growth, survival, and antioxidant response of superoxide dismutase (sod) in juvenile redclaw cherax quadricarinatus. six practical diets containing three crude protein (cp) levels (28, 35, and 40%) and two energy levels (~18 and 20 kj · g-1) were tested. the control group was fed a commercial shrimp diet. the highest sod activity (34.8 u·mg-1 protein) occurred in the diet with 35% cp and 18 kj g-1. soluble protein in tissue of redclaw fed 28 and 35% cp increased, compared to the control. the best mean final weight (9.23 g), weight gain (788%), and the lowest feed conversion ratio (1.3) occurred with the 35% cp diet. we conclude that a dietary cp content of 35% and 18 kj · g-1 is recommended for production and best immune responses in redclaw.
Comparative Study of the Fatty Acid Binding Process of a New FABP from Cherax quadricarinatus by Fluorescence Intensity, Lifetime and Anisotropy  [PDF]
Jiayao Li, Etienne Henry, Lanmei Wang, Olivier Delelis, Huan Wang, Fran?oise Simon, Patrick Tauc, Jean-Claude Brochon, Yunlong Zhao, Eric Deprez
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0051079
Abstract: Fatty acid-binding proteins (FABPs) are small cytosolic proteins, largely distributed in invertebrates and vertebrates, which accomplish uptake and intracellular transport of hydrophobic ligands such as fatty acids. Although long chain fatty acids play multiple crucial roles in cellular functions (structural, energy metabolism, regulation of gene expression), the precise functions of FABPs, especially those of invertebrate species, remain elusive. Here, we have identified and characterized a novel FABP family member, Cq-FABP, from the hepatopancreas of red claw crayfish Cherax quadricarinatus. We report the characterization of fatty acid-binding affinity of Cq-FABP by four different competitive fluorescence-based assays. In the two first approaches, the fluorescent probe 8-Anilino-1-naphthalenesulfonate (ANS), a binder of internal cavities of protein, was used either by directly monitoring its fluorescence emission or by monitoring the fluorescence resonance energy transfer occurring between the single tryptophan residue of Cq-FABP and ANS. The third and the fourth approaches were based on the measurement of the fluorescence emission intensity of the naturally fluorescent cis-parinaric acid probe or the steady-state fluorescence anisotropy measurements of a fluorescently labeled fatty acid (BODIPY-C16), respectively. The four methodologies displayed consistent equilibrium constants for a given fatty acid but were not equivalent in terms of analysis. Indeed, the two first methods were complicated by the existence of non specific binding modes of ANS while BODIPY-C16 and cis-parinaric acid specifically targeted the fatty acid binding site. We found a relationship between the affinity and the length of the carbon chain, with the highest affinity obtained for the shortest fatty acid, suggesting that steric effects primarily influence the interaction of fatty acids in the binding cavity of Cq-FABP. Moreover, our results show that the binding affinities of several fatty acids closely parallel their prevalences in the hepatopancreas of C. quadricarinatus as measured under specific diet conditions.
Oxidative damage in tissues of juvenile crayfish (Cherax quadricarinatus von Martens, 1868) fed with different levels of proteins and lipid
Zenteno-Savín, Tania;Cortes-Jacinto, Edilmar;Vázquez-Medina, José Pablo;Villarreal-Colmenares, Humberto;
Hidrobiológica , 2008,
Abstract: this experiment investigated the effect of dietary protein and lipid levels on superoxide radical production and lipid peroxidation in juvenile redclaw crayfish, cherax quadricarinatus. nine practical diets were formulated to contain a combination of three crude protein (cp) (26, 31, and 36%) and three crude lipid (cl) (4, 8, and 12%) levels. four replicate groups of 15 crayfish (0.71 ± 0.13 g) per diet treatment were stocked in 40 l tanks, at 28 °c for 60 days. the control group was fed with a commercial shrimp diet. after the feeding period, superoxide radical (o2-) production and lipid peroxidation, measured as thiobarbituric acid reactive substances (tbars) of muscle, digestive gland and gill were analyzed. in the group fed the control diet, o2- production and tbars levels were significantly higher in the digestive gland than in muscles or gills. there was no effect of dietary protein or lipid level on o2- production in the digestive gland, muscle, and gill. however, dietary protein level significantly affected tbars levels in crayfish gills (p < 0.05). the results suggest tissue-specific effects of dietary protein and lipid levels on indicators of oxidative stress in redclaw. results indicate that a diet containing 31% cp and 8% cl provided adequate amounts of protein and lipid to satisfy nutritional requirements for optimal growth, while preventing diet-induced oxidative stress and protecting the integrity of the immune function.
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