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Posible presencia de un gen R1 en germoplasma de Solanum tuberosum Grupo Phureja
Ballesteros,Diana Carolina; Gómez,Gustavo; Delgado,María Cecilia; álvarez,María Fernanda; Juyó,Deissy; Cuéllar,David; Mosquera,Teresa;
Agronomía Colombiana , 2010,
Abstract: phytophthora infestans causes late blight in potato. this disease is the most destructive worldwide. developing cultivars resistant to the pathogen is the most important strategy for its control, so identification of resistance genes (rpi) is fundamental to support breeding programs. introgression of rpi from wild or native species within cultivars can be a good way to develop resistant cultivars. s. phureja has important culinary and nutrional traits and has been identified as a source of resistance to late blight. 88 accessions of s. phureja were genotypically characterized with molecular scar markers, the resistance gene r1 for p. infestans, the candidate gene staos2 and a caps marker linked to loci for resistance to p. infestans in solanum tuberosum. polymorphism was presented in prp1 and r1 markers. the r1 marker amplification showed the 1,400 pb allele, which represents the r1 gene allele in 17 s. phureja accessions suggesting that these accessions could possibly have a homologous gene to the r1 gene identified in s. demissum
Genome-Wide Identification and Mapping of NBS-Encoding Resistance Genes in Solanum tuberosum Group Phureja  [PDF]
Roberto Lozano, Olga Ponce, Manuel Ramirez, Nelly Mostajo, Gisella Orjeda
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034775
Abstract: The majority of disease resistance (R) genes identified to date in plants encode a nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain containing protein. Additional domains such as coiled-coil (CC) and TOLL/interleukin-1 receptor (TIR) domains can also be present. In the recently sequenced Solanum tuberosum group phureja genome we used HMM models and manual curation to annotate 435 NBS-encoding R gene homologs and 142 NBS-derived genes that lack the NBS domain. Highly similar homologs for most previously documented Solanaceae R genes were identified. A surprising ~41% (179) of the 435 NBS-encoding genes are pseudogenes primarily caused by premature stop codons or frameshift mutations. Alignment of 81.80% of the 577 homologs to S. tuberosum group phureja pseudomolecules revealed non-random distribution of the R-genes; 362 of 470 genes were found in high density clusters on 11 chromosomes.
Heredabilidad del contenido de proteína total en papa diploide Solanum tuberosum grupo Phureja
Caicedo, Daniel Rodríguez;López, Carlos Eduardo ?ustez;Torres, Jose Miguel Cotes;Molano, Luis Ernesto Rodríguez;
Bragantia , 2011, DOI: 10.1590/S0006-87052011000400005
Abstract: in recent years, genetic studies that supports the plant breeding program for improve the nutritional value of potato tuber have been very important. solanum tuberosum group phureja is a species with high potential for exportation and better nutritional characteristics than species from other groups. in this research we estimate de heritability by variance components based on analysis of variance, parent offspring regression, restricted maximum likelihood and bayesian estimation. the total protein content was assessed in 103 sib maternal families. for this purpose we determined the total nitrogen by the kjeldahl method and the protein conversion factor was used. the estimates were 0.35, 0.28, 0.40 and 0.41 respectively. according to the heritability estimates and supposing a moderate selection pressure (20%), increments in protein content from0.55% to 0.78% could be obtained by each selection cycle.
Posible presencia de un gen R1 en germoplasma de Solanum tuberosum Grupo Phureja  [cached]
Ballesteros Diana carolina,Gomez gustavo,Delgado Maria Cecilia,Alvarez Maria Fernanda
Agronomía Colombiana , 2010,
Abstract: Phytophthora infestans causa la gota, que es la enfermedad en la papa de mayor importancia económica a nivel mundial. La obtención de cultivares resistentes al patógeno es una estrategia necesaria para su control, por lo cual la identificación de genes de resistencia a P. infestans (Rpi) es fundamental para apoyar los programas de mejoramiento. La introgresión de Rpi desde especies silvestres o nativas dentro de cultivares es una vía para desarrollar cultivares con resistencia. S. phureja tiene notables características culinarias y nutricionales, e igualmente ha sido identificada como una fuente de resistencia a la gota, lo cual la sitúa en una posición de interés desde el punto de vista de recurso genético con fines de mejoramiento. Se caracterizaron genotípicamente 88 accesiones de S. phureja con marcadores moleculares tipo SCAR, el gen R1 de resistencia a P. infestans, el gen candidato StAOS2 y un marcador tipo CAPS ligados a loci para resistencia a P. infestans en Solanum tuberosum. Se presentó polimorfismo en los marcadores Prp1 y R1. La amplificación del marcador R1 mostró el alelo de 1.400 pb, característico del gen R1 en 17 accesiones de la colección de S. phureja, lo cual sugiere que estas accesiones posiblemente podrían tener un gen homólogo al gen R1 identificado en S. demissum.
Comparación cuantitativa de ácido abscísico y citoquininas en la tuberización de Solanum tuberosum L. y Solanum phureja Juz. et Buk.
Ortiz,Luz Yineth; Flórez,Víctor Julio;
Agronomía Colombiana , 2008,
Abstract: of all 30 potato varieties cultivated in colombia only 10 possess a commercial importance. among other factors, this number is limited by the action of endogenous hormones that accelerate different phases of tuberization process, especially in the earliest species. in this work, the samples of tubers from different potato species solanum tuberosum l. vars. ica-unica, tuquerre?a and solanum phureja juz. et buk. var. yema de huevo were collected at the phenological stages of development 03, 10 and 40. later, the concentrations of abscisic acid in the acid fraction and ones of cytokinins in the organic fraction of the extracts were determined by high performance liquid chromatography. the results in studied varieties show a relationship between the early tuberization and aba absence, while the periods of deep latency were related with aba presence; otherwise, the cytokinin levels were high in the varieties with early tuberization. that is to say, it was found in s. phureja juz. et buk. var. yema de huevo, characterized by early tuberization, low aba concentrations and considerable levels of cytokinins, whereas, in varieties with periods of prolonged latency, such as ica-unica and, especially, tuquerre?a, aba concentrations were considerable, but those of cytokinin not.
Cinética de Inactivación de la Enzima Peroxidasa, Color y Textura en Papa Criolla (Solanum tuberosum Grupo phureja) sometida a tres Condiciones de Escaldado
Mendoza,Rolando; Herrera,Aníbal O;
Información tecnológica , 2012, DOI: 10.4067/S0718-07642012000400009
Abstract: the objective of this work was to determine the inactivation kinetics of peroxidase enzyme and the change in color and texture in golden potato tubers (solanum tuberosum phureja group) exposed to blanching with water at 80°c, 90°c and to saturated steam at 93°c. the heat transfer coefficients for the blanch water ranged between 214 and 230 w °c-1 m-2 while for saturated steam they showed an average of 84.5 w °c-1 m-2. at different pre-cooking times the golden potato tuber showed an area that developed enzymatic browning, which was fitted to the michaelis-menten kinetic model and another area where the color change decreased due to enzyme inactivation, which corresponded to first order kinetics, in a similar way as the texture behavior. the inactivation of peroxidase enzyme followed the lumry-eyring mechanism.
Comparación cuantitativa de ácido abscísico y citoquininas en la tuberización de Solanum tuberosum L. y Solanum phureja Juz. et Buk.  [cached]
Ortiz Luz Yineth,Flórez Víctor Julio
Agronomía Colombiana , 2008,
Abstract: De las 30 variedades de papa cultivadas en Colombia tan solo 10 poseen importancia comercial. Entre otros factores, este número es limitado por la acción de hormonas endógenas que aceleran diferentes fases del proceso de tuberización, especialmente en las especies más precoces. En este trabajo fueron recolectadas muestras de tubérculos de diferentes especies de papa (Solanum tuberosum L. var. ICA-única y Tuquerre a, y Solanum phureja Juz. et Buk. var. Yema de Huevo), en los estadios fenológicos de desarrollo 03, 10 y 40. Posteriormente, las concentraciones de ácido abscísico (ABA) presentes en la fracción ácida y de citoquininas presentes en la fracción orgánica de los extractos fueron determinadas a través de Cromatografía Líquida de Alta Eficiencia. Los resultados en las variedades estudiadas relacionan la precocidad de la tuberización con la ausencia de ABA y los periodos de mayor latencia con su presencia; por otro lado, las concentraciones de citoquininas fueron evidentes en las variedades con mayor precocidad. Es decir, en S. phureja Juz. et Buk. var. Yema de Huevo se encontraron concentraciones bajas de ABA y altas de citoquininas; mientras que en ICA-única y, en especial, en Tuquerre a, variedades con periodos de latencia mayor, las concentraciones de ABA fueron altas y las de citoquininas menos significativas.
Cinética de Inactivación de la Enzima Peroxidasa, Color y Textura en Papa Criolla (Solanum tuberosum Grupo phureja) sometida a tres Condiciones de Escaldado Kinetics of Peroxidase Enzyme Inactivation, Color and Texture in Golden Potato (Solanum tuberosum phureja group) under three Blanching Conditions  [cached]
Rolando Mendoza,Aníbal O Herrera
Información Tecnológica , 2012,
Abstract: El objetivo de este trabajo fue determinar la cinética de inactivación de la enzima peroxidasa y la cinética del color y textura en tubérculos de papa criolla (Solanum tuberosum grupo phureja) sometidos a escaldado mediante agua a 80°C, 90°C y vapor saturado a 93°C. Los coeficientes de transferencia de calor para el escaldado en agua oscilaron entre 214 y 230 W °C-1 m-2 mientras que para vapor saturado fueron en promedio 84.5 W °C-1 m-2. A diferentes tiempos de precocción el tubérculo de papa criolla presentó una zona en donde ocurrió pardeamiento enzimático, hecho que fue modelado según la cinética de Michaelis-Menten y otra zona en donde el cambio de color disminuyó debido a la inactivación enzimática que correspondió a una cinética de primer orden, de manera similar al comportamiento de la textura. La inactivación de la enzima peroxidasa obedeció al mecanismo de Lumry-Eyring. The objective of this work was to determine the inactivation kinetics of peroxidase enzyme and the change in color and texture in golden potato tubers (Solanum tuberosum phureja group) exposed to blanching with water at 80°C, 90°C and to saturated steam at 93°C. The heat transfer coefficients for the blanch water ranged between 214 and 230 W °C-1 m-2 while for saturated steam they showed an average of 84.5 W °C-1 m-2. At different pre-cooking times the golden potato tuber showed an area that developed enzymatic browning, which was fitted to the Michaelis-Menten kinetic model and another area where the color change decreased due to enzyme inactivation, which corresponded to first order kinetics, in a similar way as the texture behavior. The inactivation of peroxidase enzyme followed the Lumry-Eyring mechanism.
Assessment of the processing profile of six “creole potato” genotypes (Solanum tuberosum Phureja Group)  [cached]
Rivera Jesús Elías,Herrera Anibal Orlando,Rodríguez Luis Ernesto
Agronomía Colombiana , 2011,
Abstract: Six diploid potato (“creole potato”, Solanum tuberosum PhurejaGroup) genotypes were grown at the localities of Soacha, Duitama and Mosquera (Colombia) and were assessed for their suitability for industrial processing using Individual Quick Freezing (IQF) as pickled and dehydrated (flakes) potatoes. The following variables were assessed: Percentage of defective tubers, dry weight, specific gravity, “eye” depth, skin type, shape, appearance, color, aroma, flavor and texture. Cultivar Criolla Colombia and clone 98-71.26 showed the best behavior for precooking processing. The best characteristics for IQF and pickling corresponded to tubers with yellow colored peel, round shape, shallow “eyes”, 16 to 20.5% dry weight, 2.5 to 3.5 cm diameter and less than 0.1% reducing sugars. For dehydrated flakes the tubers with 21-25% dry weight, large size and reducing sugars below 0.1% exhibited the best processing behavior. In addition, it was observed that the cultivar’s environmental conditions affect tuber quality and processing type to be performed.
Propuesta de un sistema de transformación de plantas de papa (Solanum tuberosum sp. andigena var. Pastusa suprema) mediado por Agrobacterium tumefaciens
López,Alfredo; Chaparro,Alejandro;
Agronomía Colombiana , 2007,
Abstract: it has been demonstrated that agrobacterium tumefaciens mediated-transformation of potato (solanum tuberosum) was depended upon the genotype. in addition, most of the proposed protocols were inefficient to transform the andigena subspecies. in this proposal, the initial processes of genetic improvement of the new colombian variety pastusa suprema, which highly andro-sterile characteristic is of great importance for genetically modified organisms, were handled. this variety was produced across the inter-specific hybridization of three different species of potato (solanum stoloniferum, solanum phureja var. yema de huevo y solanum tuberosum sp. andigena var. parda pastusa). stem explants was transformed using the vector pcambia 2301, which has the gene reporter of β-glucoronidase and the gene of resistance to kanamycin. percentage initial transformation was expressed as formation of callus on a selection medium of 31 ± 2.5% with a final 30% frequency using gus assay. it is the first report of a transformation process of an inter-specific hybrid derived from three different species.
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