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Comparative HPTLC fingerprint profile of Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms fruits  [PDF]
Saraswathy A,Vidhya B,Amala K
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: Objective: To establish the fingerprint profile of two Illicium species namely Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms using high performace thin layer chromatography (HPTLC) technique. Methods: CAMAG HPTLC system equipped with TLC autosampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software was used. n-hexane, chloroform and ethyl acetate extracts of the fruits were developed in suitable mobile phase using standard procedures and scanned under UV at 254nm, 366 nm and under visible light. Results: The HPTLC fingerprinting of the n-hexane, chloroform and ethyl acetate extracts showed several peaks with different Rf values. Conclusion: HPTLC fingerprint profile of two Illicium species may be useful in differentiating the species and also helpful in the identification and authentication of these species
HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa  [PDF]
Daya L. Chothani,M. B. Patel,S. H. Mishra
Chromatography Research International , 2012, DOI: 10.1155/2012/180103
Abstract: The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366?nm) at : 0.56 (mobile phase chloroform?:?toluene?:?ethyl acetate (6?:?3?:?1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366?nm) at : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound. 1. Introduction Marker compound means chemical constituents within a medicinal that can be used to verify its potency or identity. For sometimes, the marker compounds may be described as active ingredients or chemicals that confirm the correct botanical identity of the starting material. It is very difficult to identify correct marker compounds for all traditional medicinals, because some medicinals have unknown active constituents and others have multiple active constituents. A chromatographic fingerprint of a herbal medicine is a chromatographic pattern of the extract of some common chemical components of pharmacologically active and/or chemical characteristics. By using chromatographic fingerprints, the authentication and identification of herbal medicines can be accurately conducted even if the amount and/or concentration of the chemically characteristic constituents is not exactly the same for different samples of drug. Hence it is very important to obtain reliable chromatographic fingerprints that represent pharmacologically active and chemically characteristic component of the herbal drug [1–5]. Ruellia tuberosa is an erect, suberect, or diffuse perennial herb up to 60–70?cm tall herb and belongs to family Acanthaceae, a native of Central America, introduced into Indian garden as ornament. It is used medicinally in West Indies, Central America, Guiana, and Peru. R. tuberosa is commonly known as “Cracker plant” [6–8]. In Siddha system of medicine, leaves are given with liquid copal as remedy for gonorrhea and ear diseases [9], used in stomach cancer [10]. Dried and ground roots in dose of two ounces cause abortion and also used in sore eyes [11]. The herb also exhibits emetic activity and employed substitute of ipecac, also used in bladder
Preeti Tiwari* and Rakesh K. Patel
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta–T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of gallic acid in Arjunarishta-T, M and its marketed formulation was found to be 0.0332, 0.0331 and 0.0330% w/w respectively while ellagic acid was found to be 0.0361, 0.0360 and 0.0359% w/w respectively. This is the first report for the quantification of gallic acid and ellagic acid in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and ellagic acid from Arjunarishta.
Validated HPTLC Fingerprint Analysis for Simultaneous Estimation of Quercetin, Kaempferol and Asiatic Acid in Leaves of Two Different Chemotypes of Centella asiatica
C Joshi, J Savai,A Varghese,N Pandita
International Journal of Pharmaceutical and Biological Research , 2012,
Abstract: Quercetin, Kaempferol and Asiatic acid in the methanolic extract of leaves of Two Chemotypes of Centella asiatica was developed and validated. Methods: The HPTLC method was developed using a suitable mobile phase Toluene: Ethyl acetate: Chloroform: Formic acid (6:6:4:1). The plates were derivatized with anisaldehyde reagent and densitometric determination was performed at wavelength 530nm.Results: The Rf values for Asiatic acid, Quercetin, and Kaempferol was 0.33, 0.44 and 0.55 respectively. The linear regression analysis for the calibration showed a linear relationship with r2= 0.9764 and 0.9823 for Quercetin and Kaempferol respectively in the concentration range of 100-1000ng/band. In case of Asiatic acid, the linear regression analysis data for the calibration plots showed a linear relationship with r2=0.9996 in the concentration range of 100-500ng/band. Precision was confirmed by performing replicate analysis on a single day and different days. The accuracy of the method was validated by conducting recovery studies using thestandard addition method. The average recovery for Quercetin was 98.60% for Kaempferol was 90.08% and for Asiatic acid was 97.66%. System suitability parameter was evaluated by spotting five replicates of all three standards. The %RSD was found to be below 2% with respect to peak area as well as Rf value. Robustness ofmethod was evaluated by changing mobile phase concentration from Toluene: Ethyl Acetate: Chloroform: Formic Acid (6:6:4:1) to (5:5:5:1), for which %RSD of peak areas was found to be below 10%.Conclusion: The HPTLC method developed was found to be simple, precise, accurate, suitable and robust for routine quality control of the powder of leaves of Centella asiatica.
Chromatographic finger print analysis of budmunchiamines in Albizia amara by HPTLC technique
T. Rajkumar, B.N.Sinha
International Journal of Research in Pharmaceutical Sciences , 2010,
Abstract: Recently, there has been an increase in the use of herbal drugs for healthcare. Herbal Medicine is a time-tested and valuable resource for healing. In India, the herbal drug market is about $ one billion and the export of plant based crude drugs is around $ 80 million. So it is absolutely essential to provide scientifically proven and evidence based herbal formulations for global acceptance. Chromatographic fingerprint symbolize the active chemical constituents of herbal medicines for desired therapeutic action. This study presents a simple, rapid and selective HPTLC method for separation and determination of Albizia amara. Albizia amara (Fabaceae) is the medicinal plant which has been used to treat piles, diarrhoea, gonorrhoea, leprosy, and leucoderma. The macro cyclic alkaloid Budmunchiamines is the main constituent which has been separated and identified by camag HPTLC. The coarsely dried powdered leaves were extracted with Petroleum Ether (60-80)0C by cold maceration, and hot percolation with 90% methanol for 72 h. TLC profile and HPTLC fingerprints were recorded by using silica gel GF254 as stationary phase. Separation was performed on pre-coated 10x10cm HPTLC Aluminium sheets. Sample volumes of (5μl) were applied to the 6 tracks contain bandwidth of 5mm and 10 secs/μl using a TLC applicator. The plates were developed using a mobile phase of Chloroform: diethylamine (86:22 v/v). The peak 2, 6 reveals that the presence of macro cyclic alkaloids Budmunchiamines L4, L5 were characterised and confirmed by FTIR, 1H NMR, MASS and CHN analysis. The results were compared with earlier reports. The developed HPTLC fingerprints will help the manufacturer for quality control and standardization of herbal formulations.
Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Extracts of Pisonea Aculeata  [PDF]
Mamoon Hussain Syed,Ayesha Yasmeen,Mohammad Suleman Hussain,N. Siva Subramanian
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: Objective: To establish the fingerprint profile of Pisonea aculeata using high performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, triterpenes, tannins, saponnins, glycosides, phenolic compounds and flavonoids. HPTLC finger printing of chloroform extract of leaf revealed 14 peaks with Rf values in the range of 0.03 to 0.95; ethyl acetate extract of leaf showed 6 peaks with Rf values in the range of 0.04 to 0.94 and 90% ethanolic extract of leaf revealed 11 peaks with Rf values in the range of 0.03 to 0.93. Conclusions: It can be concluded that HPTLC fingerprint analysis of leaf extract of Pisonea aculeata can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.
Phytochemical, HPTLC finger printing and antibacterial activity of Acacia nilotica (L.) Delile
R.Venkataswamy,M.Sukumar,A.Doss, H. Mohamed Mubarack
Hygeia : Journal for Drugs and Medicines , 2010,
Abstract: The leaves of Acacia nilotica (L.) Delile was extracted with methanol and aqueous medium and studied for in vitro antimicrobial property. The methanol extract was found to be most active against all the bacterial species tested except S.aureus. Active methanol extract was further studied for HPTLC fingerprint and by phytochemical analysis. HPTLC analysis confirmed segregation of four individual compounds with individual Rf values and peak area percentage. The results of phytochemical screening of extract revealed the presence of Tannins, Carbohydrates and Glycosides. This analysis revealed the high antibacterial activity in the methanol extract of Acacia nilotica (L.) Delile
Chemicals and Nutritional Composition of Four Botanicals with Fungitoxic Properties  [PDF]
D.A. Alabi,M.Z. Onibudo,N.A. Amusa
World Journal of Agricultural Sciences , 2005,
Abstract: Evaluation of four botanicals Vernonia amygdalina L., Bryophyllum pinnatum L., Eucalyptus globules Labill and Ocimum gratissimum Kurz (Clocimum) for chemical and nutritional properties was conducted at Ago Iwoye in South-western Nigeria. The results from the investigation showed that V. amygdalina contained large quantity of Thiamine, Pyridoxine, Ascorbic acid, Glycine, Cysteine and Casein hydrolysate significantly more than other botanicals. Similarly, all the botanicals contained carbohydrates, proteins and lipids. However, the acid contents of B. pinnatum and E. globules were significantly higher than those of V. amygdalina and O. gratissimum. Similarly, the hydrocyanic acid and oxalic acid contents of B. pinnatum and E. globules were significantly (p = 0.05) higher than those of O. gratissimum. Moreover, the four botanicals contained mineral nutrients and sugars; hence, if the botanicals were used to treat cowpea plants, all these would be supplied to cowpea plants and so enhance their performances.
Automation of Fingerprint Recognition Using OCT Fingerprint Images  [PDF]
Nasibe Akbari, Ali Sadr
Journal of Signal and Information Processing (JSIP) , 2012, DOI: 10.4236/jsip.2012.31015
Abstract: Automated recognition of a person is one of the most critical issues in the modern society. Common biometric systems rely on the surface topography of an object and, thus, are potentially vulnerable for spoofing. Optical coherence tomography is a technology that has the capability to probe the internal structure of multilayered tissues. The paper describes an algorithm for automation fingerprint recognition that the algorithm is applied on the OCT fingerprint images. This algorithm is based on scanning of the enhanced and segmented OCT images.
Determination of bacoside A by HPTLC in Bacopa monnieri extract  [cached]
Prakash Om,Singh Gyanendra,Singh Raman,Mathur Satish
International Journal of Green Pharmacy , 2008,
Abstract: A simple sensitive HPTLC method developed for the determination of bacoside A in the plant Bacopa monnieri extracts. The stationary phase was precoated silica gel GF254. The mobile phase used was dichloromethane: methanol: water (4.5: 1.0: 0.1 v/v/v). The plate was scanned and quantified at 225 nm for bacoside A. The method was validated in terms of linearity, accuracy and specificity. The proposed HPTLC method provides a faster and cost effective qualitative control for routine analysis of bacoside A in extracts containing Bacopa monnieri saponins.
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