Deng L, Wang C, Spencer E, Yang LY, Braun A, et al. (2000) Activation of the I kappa B kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 103: 351–361.
has been cited by the following article:
- TITLE: The Dark Side of EGFP: Defective Polyubiquitination
- AUTHORS: Mathijs Baens, Heidi Noels, Vicky Broeckx, Sofie Hagens, Sabine Fevery, An D. Billiau, Hugo Vankelecom, Peter Marynen
JOURNAL NAME: PLOS ONE
Aug 29, 2014
- ABSTRACT: Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect cell physiology. Thus far, the precise mechanism of GFP-associated defects remained unclear. Here we demonstrate that EGFP and EGFP fusion proteins inhibit polyubiquitination, a posttranslational modification that controls a wide variety of cellular processes, like activation of kinase signalling or protein degradation by the proteasome. As a consequence, the NF-κB and JNK signalling pathways are less responsive to activation, and the stability of the p53 tumour suppressor is enhanced in cell lines and in vivo. In view of the emerging role of polyubiquitination in the regulation of numerous cellular processes, the use of EGFP as a live cell reporter should be carefully considered.