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- TITLE: Development of a drtqPCR Assay for Differentiation of PCV-2a and 2b in Shanghai Area
- AUTHORS: Jian Liu, Feifei Ge, Houbing Ju, Dequan Yang, Xin Li, Jian Wang, Peihong Liu, Jinping Zhou
- KEYWORDS: Genotype Differentiation, drtqPCR, PCV-2
JOURNAL NAME: Open Journal of Veterinary Medicine
Dec 13, 2014
- ABSTRACT: A dual real-time quantitative polymerase
chain reaction assay (drtqPCR) was established to detect and differentiate
between Porcine circovirus-2a (PCV-2a) and Porcine circovirus-2b (PCV-2b).
Genotype-specific primer sets and probes were designed by using sequence data
published for different PCV-2 strains. Specificity and sensitivity of the
drtqPCR were examined by using PCV-2 isolates with known genotype. Among 367
tissue samples, 44.69% (164/367) were PCV-2 positive. From 164 PCV-2 positive
samples, 10.98% (18/164), 92.56% (137/164), and 3.31% (9/164) were positive for
PCV-2a, PCV-2b, and both genotypes, respectively. These results suggest that
the dif-ferential drtqPCR can be used to detect PCV-2 and to differentiate the
2 genotypes from field sam-ples. The PCV-2 infection is quite common in swine
of Shanghai area. Furthermore, the PCV-2b infective ratio is far higher than
PCV-2a, and PCV-2a/2b mixed infections are also observed but at a lower
prevalence in Shanghai area.