文章

Guo, L.J., Lu, Y.H., Wei, Y.W., Huang, L.P. and Liu, C.M. (2010) Porcine Circovirus Type 2 (PCV2): Genetic Variation and Newly Emerging Genotypes in China. Virology Journal, 7, 273.
http://dx.doi.org/10.1186/1743-422X-7-273

被如下文章引用：

• TITLE: Development of a drtqPCR Assay for Differentiation of PCV-2a and 2b in Shanghai Area
• AUTHORS: Jian Liu, Feifei Ge, Houbing Ju, Dequan Yang, Xin Li, Jian Wang, Peihong Liu, Jinping Zhou
• KEYWORDS: Genotype Differentiation, drtqPCR, PCV-2
• JOURNAL NAME: Open Journal of Veterinary Medicine DOI: 10.4236/ojvm.2014.412035 Dec 13, 2014
• ABSTRACT: A dual real-time quantitative polymerase chain reaction assay (drtqPCR) was established to detect and differentiate between Porcine circovirus-2a (PCV-2a) and Porcine circovirus-2b (PCV-2b). Genotype-specific primer sets and probes were designed by using sequence data published for different PCV-2 strains. Specificity and sensitivity of the drtqPCR were examined by using PCV-2 isolates with known genotype. Among 367 tissue samples, 44.69% (164/367) were PCV-2 positive. From 164 PCV-2 positive samples, 10.98% (18/164), 92.56% (137/164), and 3.31% (9/164) were positive for PCV-2a, PCV-2b, and both genotypes, respectively. These results suggest that the dif-ferential drtqPCR can be used to detect PCV-2 and to differentiate the 2 genotypes from field sam-ples. The PCV-2 infection is quite common in swine of Shanghai area. Furthermore, the PCV-2b infective ratio is far higher than PCV-2a, and PCV-2a/2b mixed infections are also observed but at a lower prevalence in Shanghai area.