the objective of this study was to evaluate the effect of the application of micronutrients and growth regulators on the germination, vigor, activity of some enzymes, and on the total protein contents in tomato seeds during the storage. the seeds were treated with the chemicals starter？, cellerate？, and stimulate？ at the dosages corresponding to 0%, 50%, 100%, 150%, and 200% of the dose recommended by the manufacturer, utilizing the film-coating technique. the evaluations were performed at 0, 6, and 12 months of storage by the following parameters: percentage of germination, percentage and emergency velocity rate, activity of enzymes endo-a-mannanase and esterase, total protein content, and health. the completely randomized design in a 3 x 5 x 3 factorial structure was utilized, namely, three chemicals, five doses, and three storage periods with four replicates of 50 seeds per treatment. coating with the chemicals based on micronutrients and growth regulators and storage interfere on the activity of enzyme endo-a-mannanase in the tomatoes and seeds; the activity of enzyme esterase increases with the storage of tomato seeds, pointing to an increase in the deterioration process; there is an increase in the total protein content in tomato seeds throughout the storage period; chemicals based on growth regulators promote an increase in emergency velocity rate of the tomato seedlings when applied at pre-sowing at the recommended dose.