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Acetylcholinesterase Based Detection of Residual Pesticides on Cotton

DOI: 10.4236/ajac.2012.32013, PP. 93-98

Keywords: cetylcholinesterase, Acetylcholine, Acetylthiocholine Chloride, Biosensor Toxicity Analyzer, Cryogenic Homogenization, Soxhlet Extraction Method

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Abstract:

This study describes the measurement of bio-electrical signals caused by enzymatic inhibition of acetylcholinesterase (AChE) for the detection of organophosphorous and carbamate pesticides which are the strong inhibitors of AChE and prevents its normal function of the rapid removal of acetylcholine (Ach). Biosensor Toxicity Analyzer (BTA) was used for the testing and enzyme activity was determined by acetylthiocholine chloride (ATCCl) as enzyme substrate. The monitoring of changes in bio-electrical signals caused by the interaction of biological substances and residues were evaluated. Two samples of cotton were analyzed. Cryogenic homogenization was carried out for sample pretreatment and Soxhlet extraction method (SOX) was used for extraction. The resulted extracts were concentrated and then injected in the BTA. The method shows reasonable results and can successfully be utilized for the detection of residual pesticides on different types of cotton.

References

[1]  W. C. Robertson and B. A. Roberts, “Integrated Crop Management for Cotton Production in the 21st Century,” In: M. R. Chaudhry and P. J. Wakelyn, Eds., COTTON: Technology for the 21st Century, First, International Cotton Advisory Committee, Washington DC, 2010, pp. 63-98.
[2]  S. Stilton, D. Myers, “Organic Cotton: A More Sustainable Approach,” Organic Cotton, Intermediate Technology Publications Limited, London, 1999, pp. 1-7.
[3]  L. Grose, “Sustainable Cotton Production,” In: R. S. Blackburn, Ed., Sustainable Textiles: Life Cycle and Environmental Impact, Woodhead Publishing Limited, Cambridge, 2009, pp. 33-62.
[4]  T. E. Martín-Esteban, “Sample Handling of Pesticides in Food and Environmental Samples,” In: J. L. Tadeo, Ed., Analysis of Pesticides in Food and Environmental Samples, CRC Press, Boca Raton, 2008, pp. 35-58.
[5]  J. W. S. Hearle, “Physical Structure and Properties of cotton,” In: S. Gordon and Y.-L. Hsieh, Eds., Cotton: Science and Technology, Woodhead Publishing Limited, Cambridge, 2007, pp. 35-67.
[6]  A. Lutz, “Residue Analysis of 500 High Priority Pesticides: Better by GC-Ms or LC-Ms/Ms?” Wiley InterScience, Vol. 25, No. 6, 2006, pp. 838-865. doi:10.1002/mas.20091
[7]  A. El-Rahman El-Naggar, “Clinical Findings and Cholinesterase Levels in Children of Orga-nophosphates and Carbamates Poisoning,” European Journal of Pediatrics, Vol. 168, No. 8, 2009, pp. 951-956. doi:10.1007/s00431-008-0866-z
[8]  H. Y. Hu, “A Novel Chemiluminescence Assay of Or- ganophosphorous Pesticide Quinalphos Residue in Vegetable with Luminol Detection,” Chemistry Central Journal, Vol. 4, 2010, p. 13. doi:10.1186/1752-153X-4-13
[9]  A. Mulchandani, “Biosen-sors for Direct Determination of Organophosphate Pesticides,” Biosensors & Bioelectronics, Vol. 16, No. 4, 2001, pp. 225-230. doi:10.1016/S0956-5663(01)00126-9
[10]  M. L. Hannam, “Characterisation of Esterases as Po- tential Biomarkers of Pesticide Exposure in the Lugworm Arenicola Marina (Annelida: Polychaeta),” Environ- mental Pollution, Vol. 152, No. 2, 2008, pp. 342-350. doi:10.1016/j.envpol.2007.06.022
[11]  P. Mulchandani, “Biosensor for Direct Determination of Organophosphate Nerve Agents. 1. Potentiometric Enzyme Electrode,” Biosensors & Bioelectronics, Vol. 14, No. 1, 1999, pp. 77-85. doi:10.1016/S0956-5663(98)00096-7
[12]  R. P. Deo, “Deter-mination of Organophos-phate Pesticides at a Carbon Nano-tube/Organophosphorus Hydrolase Electrochemical Biosensor,” Analytica Chimica Acta, Vol. 530, No. 2, 2005, pp. 185-189. doi:10.1016/j.aca.2004.09.072
[13]  D. G. Buerk, “Biosensors: Theory and Applications,” 1st Edition, Technomic Publishing Company, Lancaster, 1995.
[14]  A. Adina. “Development of a Pesticides Biosensor Using Carbon-Based Electrode Systems,” In: L. Simeonov and E. Chirila, Eds., Chemicals as Intentional and Accidental Global Environmental Threats, Springer, Dor-drecht, 2006, pp. 337-343.
[15]  J. L. Tadeo, “Pesticides: Classi?cation and Properties,” In: J. L. Tadeo, Ed., Analysis of Pesticides in Food and Environmental Samples, CRC Press, Boca Raton, 2008, pp. 1-34.
[16]  A. Mulchandani, “Microbial Biosensors for Organophosphate Pesticides,” Applied Bioche-mistry and Biotechnology, Vol. 165, No. 2, 2011, pp. 687-699. doi:10.1007/s12010-011-9288-x

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