purpose: to evaluate anatomical and functional results of 25-gauge transconjunctival sutureless pars plana vitrectomy in patients with uveitis. methods: vitrectomy was performed on 20 eyes with residual vitritis secondary to infectious and noninfectious uveitis. patients were evaluated 1 week before surgery and after surgery at day 1, week 1, week 4 and week 12. visual acuity (va), intraocular pressure, anterior chamber cells and flare and vitreous haze were measured. results: mean va improved from 2.06 ± 0.94 logmar before surgery to 0.58 ± 0.46 logmar at week 12 (p<0.05). no case required conversion to standard 20-gauge instrumentation or suture placement, no intraoperative complications were noted. transient postoperative hypotony was seen in three eyes. one patient with toxoplasmic retinochoroiditis had a relapse during follow-up. conclusion: 25-gauge vitrectomy has proven its efficacy on cleansing vitreous opacities and improving visual acuity on patients with residual vitritis secondary to uveitis with minimal postoperative inflammation and complications.