the purpose of this research was to evaluate the concentration of tryglicerides, cholesterol, aspartate transaminase (ast) and progesterone (p4) in embryo donor nelore cows superovulated with different protocols. twenty four donors were randomly distributed in three groups: group 1 (n=11), donors superovulated with 500ui of fsh and group 2 (n=8) and group 3 (n=5) respectively with 200mg and 180mg of fsh, in decreasing doses, twice a day, during four consecutive days. blood samples were collected before superovulation (a), in the third day of superovulation (b), at the artificial insemination time (c) and at the embryo collection time (d). the concentrations of tryglicerides, aspartate transaminase (ast) and cholesterol were measured by spectrophotometry and progesterone (p4) by radioimmunoassay. there was no alteration (p<0.05) in the concentration of tryglicerides, ast and cholesterol among the samples. there was no effect (p>0.05) of the superovulation protocol on the concentration of tryglicerides, ast and p4 in the samples. in the samples a and b of group 2 the concentration of cholesterol was lower (p<0.05) than in groups 1 and 3, probably due to the instability of the parameter.