during a six-year period from 2000 to 2006, 257 animal samples (fur and skin desquamation) were submitted to mycological examination in the laboratory of microbiology at the universidade de trás-os-montes e alto douro, vila real, portugal. dermathophytes were cultured from 38 of 257 (14.8%) specimens. chi-squared test was used to compare differences between independent groups. the most frequent isolated dermathophyte was microsporum canis (47.4%). other isolated dermathophytes were: trichophyton mentagrophytes var. mentagrophytes (31.5%), microsporum gypseum (15.8%), and microsporum nanum (5.3%). the rates of dermathophytes isolation were 8.4% in dogs; 21.3% in cats; 66.7% in rabbits; and in 11.1% in horses.