the objective was to evaluate the effects of temperature on performance and activity of lipase in the nile tilapia, thai lineage. the experimental design was completely randomized with four treatments (20, 24, 28 and 32oc), six replicates and ten fishes per experimental unit. the diet was the same for all treatments. at 55 days of experiment were evaluated apparent feed intake, weight gain, apparent feed conversion, lipase activity and specific lipase activity. the apparent feed intake and weight gain increased linearly with temperature increase. for apparent feed conversion, quadratic effect was observed as a function of temperature, showing a conversion improvement of 1.79 to 1.00 with the increase of the temperature until 29,15oc. linear effect in lipase and specific lipase activities was observed too as a result of temperature, comprising high lipase activity at 32oc. water temperature influences the performance and amylase activity in nile tilapia.