with the discovery of the human immunodeficiency virus (hiv) in 1981, the frequency of the opportunistic infections in the immunosuppressed patients, especially the infected ones by the hiv, it has experienced a notable increase. inside these there are those of micotic aetiology, which present a high grade of mortality, if they are not diagnosed and treated on time. in our country with the instauration of the highly effective antirretroviral therapy since the year 2001, a significant reduction has been seen in the morbidity and the mortality of the opportunistic infections, specialle those caused by fungus, in this type of patients. in the present study the positividad was analyzed in the mycological diagnosis realized by conventional methods of the different clinical samples received in the national laboratory of reference of mycology, from patients infected with hiv/aids during the period between january 2005 and december 2009. of a whole of 1173 biological samples processed only 324 turned out positive, for 27, 6 % of positivity, being the oropharingeal candidiasis and the cryptococcal meningoencefalitis, those of major incidence observed. in the clinical samples analyzed, a concordance of 56,2 % was obtained in the positividad of the samples studied by means of direct examination and mycologycal cultures, which confirms the importance of direct preparations of clinical samples for the visualization of fungal elements from clinical samples.