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MICROPROPAGACIóN DE PILóN (HIERONYMA ALCHORNEOIDES)

Algunos aspectos de la morfología radicular de Hieronyma alchorneoides (Pilón)

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Adapta o de espécies arbóreas nativas em um sistema agrossilvicultural, submetidas a extremos climáticos de geada na regi o de Florianópolis

Wood characteristics of Terminalia amazonia, Vochysia guatemalensis and Hyeronima alchorneoides planted in Costa Rica

Adapta??o de espécies arbóreas nativas em um sistema agrossilvicultural, submetidas a extremos climáticos de geada na regi?o de Florianópolis

Modelos para estimar la biomasa de especies nativas en plantaciones y bosques secundarios en la zona Caribe de Costa Rica

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Micropropagación de Pilón (Hieronyma Alchorneoides)

Keywords: micropropagation, in vitro culture, pilon, hieronyma alchorneoides, cytokinins.

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Abstract:

pilon (hieronyma alchorneoides) (euphorbiaceae) is one of the native timberspecies of costa rica best adapted to conditions of open plantation. due to its growth characteristics and variety of uses, the demand for planting material has increased. this species presents serious problems with seed reproduction; it is adioecious, the fruit production varies overtime and between trees, trees produce largequantities of fruit but are heavily predated by different birds. in recent years, the percentage of damaged fruit has increased due to wasps and the seeds lose their ability to germinate within days of harvest (recalcitrant). thes factorsmake micropropagation an attractive option for masspropagation of the species. in the present study various stages of in vitro cultivation were evaluated. the aseptic establishment of theembryos was achieved by scarifying the seeds with concentrated sulfuric acid for 10 min, followed by incubation in 5.5% sodium hypochloride (naclo) for 30 min, then embryos were inoculated on the culture medium described by murashige and skoog (ms) without growth regulators. when vegetative material was used as initial explant, the use of 0.095% mercuric chloride (hgcl2) during 5 min, allowed 50% of cuttings to develop shoots under aseptic conditions on a simple ms medium. it was observed that pilon does not require the addition of growth regulators during bud induction nor in the multiplication stages. during rooting, the addition of indole butyric acid (iba) at concentrations of 0.1 mg.l-1 promoted the highest percentage of rooted shoots (80%), the largest number of roots per stem (5.0) and the best quality of the roots formed. survival of in vitro plants after acclimation under greenhouse condition reached 70%

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