sixty two virus-free accessions of solanum phureja from the colombian central collection of this tuber (bogotá-soacha) were tested for their susceptibility to infection with potato yellow vein virus (pyvv). under greenhouse conditions, the plants were inoculated with pyvv using viruliferous trialeurodes vaporariorum (westwood) whiteflies, and monitored for three months (the tuber formation period of s. phureja) for symptoms of infection and virus capsid protein (cp) production, by means of a reverse transcription-polymerase chain reaction (rt-pcr) assay. symptoms of pyvv infection, consisting in the chlorotic flecking of leaflets, followed by complete vein yellowing, were noted in 38 accessions whose rna extracts generated correct-sized cp amplicons in rt-pcr assays. thirteen of the remaining accessions were found to be latently infected with the virus through positive rt-pcr assays. twelve accessions were free of virus infection as judged by the absence of symptoms and negative rt-pcr assay results over two cycles of tuber setting and germination. these accessions may possess field resistance to pyvv. this work is the first one to detect and define the levels of susceptibility of colombian s. phureja accessions to pyvv infection, and to identify the potential existence of resistance to this deleterious andean potato virus.