the objective of this study was to evaluate the effects of poultry litter and organosuper？ with three modes of application on the biomass production and nutrient content of the leaves of roselle plants. the treatments in each crop cycle were in a factorial arrangement, 2 x 3 + 1, composed of a control and combinations of the two organic fertilizers (poultry litter (10 ton. ha-1) and organosuper？ (10 ton. ha-1) and the three application modes (surface, incorporated and surface + incorporated), in a randomized block design with four replicates. in the surface + incorporated mode, the organic fertilizers were applied as 5 ton. ha-1 surface and 5 ton. ha-1 incorporated. the highest productions of fresh and dry weight and number of calyxes were obtained for poultry litter in surface (10,776, 1,239 and 3,980,602 kg ha-1, respectively) and organosuper？ incorporated (11,372, 1,308 and 4,405,075 kg ha-1, respectively) in the agricultural year 2009/2010. the increases in the fresh and dry weights of the calyxes, leaves, stems and roots, number of calyxes, leaf area and fibers in the agricultural year 2008/2009 in the poultry litter treatments. nutrients concentrations in the dry weight of the roselle leaves were not affected by the organic fertilizer type or by the mode of application.