hevea brasiliensis rubber plants were inoculated with native arbuscular mycorrhizal fungi (amf) during their greenhouse acclimatization. the amf were multiplied for 4 months associated with lolium sp. plants having 73% root colonization. seven morphotypes were obtained. two different groups of h. brasiliensis plants were inoculated with these morphotypes to determine amf effect on mortality rate, growth, mycorrhization and leaf p content: those produced in vivo by seed germination and those produced in vitro by micropropagation. plantlets produced in vitro returned 12.6% amf colonization and 0.41 g accumulated dry biomass, whereas seedlings produced in vivo had 44.7% and 1.40 g , respectively, compared to control plants with no amf inoculation which accumulated 0.37 g (in vitro) and 0.40 g (in vivo). amf inoculation reduced the plants？ mortality rate regarding in vitro produced material; however, it did not influence foliar p content. inoculated plants？ growth response was different, depending on plant material and the kind of inoculum used (native or foreign). symbiosis developed between amf and h. brasiliensis in controlled growth conditions although its advance was limited by the plants？ growth stage. nevertheless, such association influenced inoculated plants？ biomass accumulation and decreased their mortality rate and could be used as an alternative treatment during the earliest stages of h. brasiliensis plantlet production.