in greenhouse potato cultivation, mineral nutrition is one of the main factors contributing to high yields and better product quality. knowledge about the amount of nutrients accumulated in the plants at each growing phase provides important information that helps the establishment of a more balanced fertilizer application. the objective of this research was to determine the time course of macronutrients uptake and accumulation in potato plants for seed-tuber production, grown in nutrient solution. the experiment was carried out in a greenhouse, using in vitro material from the pre-basic category of the ？atlantic？ variety. the plants were collected weekly from 14 days after transplanting (dat) until 70 dat. the experimental design was a completely randomized block with 9 treatments to sampling times and four replicates. the highest nutrient requirement in the plant shoot occurred at the periods between 28 and 56 dat, while in the tubers it was after 49 dat. the maximum accumulation sequence of macronutrients was k>n>s>ca>p>mg.