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-  2018 

高血压病血瘀证患者血清对内皮细胞功能及AMPK激活的影响
Effects of the serum of hypertensive patients with blood stasis syndrome on function and AMPK activation of endothelial cells

DOI: 10.11778/j.jdxb.2018.03.013

Keywords: 高血压病,血瘀证,内皮细胞功能,炎症反应,蛋白激酶
hypertension
,blood stasis syndrome,endothelial function,inflammation,AMPK

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Abstract:

摘要 目的: 探索高血压病血瘀证患者血清对内皮细胞功能及AMPK活性的影响.方法:高血压病血瘀证患者血清刺激人脐静脉内皮细胞(HUVECs)24 h,以高血压病非血瘀证患者血清作为对照.采用一氧化氮(NO)、内皮素(ET-1)检测试剂盒检测NO和ET-1的含量.Western blot法检测磷酸化一氧化氮合酶(eNOS)、总eNOS、细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、磷酸化AMPK及总AMPK的表达.定量PCR法检测肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)的表达.结果:高血压病血瘀证患者血清处理24 h后,显著降低NO含量、抑制eNOS的活性、升高ET-1的含量,升高ICAM-1、VCAM-1的表达,升高单核细胞黏附率,升高内皮细胞TNF-α、IL-1β的表达水平,与高血压病非血瘀证患者血清相比,有统计学差异(P<0.05).此外,高血压病血瘀证患者血清显著降低蛋白激酶AMPKα的磷酸化水平,与高血压病非血瘀证患者血清相比,有统计学差异(P<0.05).结论:高血压病血瘀证患者血清能诱导内皮功能障碍和炎症反应,其机制可能与抑制AMPK的活性有关.

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