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-  2015 

纤维素酶对柠条粉基质化静态堆腐发酵效果的影响
Effect of Cellulose on Fermentation of Caragana korshinskii Powder as Substrate during Composting in Static State

DOI: 10.7606/j.issn.1004-1389.2015.07.025

Keywords: 纤维素酶 柠条 基质 发酵 酶活性 微生物种群数量
Cellulose Caragana korshinskii Substrate Fermentation Enzyme activity Microorganism species and quantity

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Abstract:

为了加快柠条粉发酵速度,缩短柠条粉发酵时间,以纤维素酶为发酵助剂,设置0.125、0.25、0.5、1.25、2.5、0 kg·m-3(CK)6个处理,通过添加纤维素酶,观测不同剂量纤维素酶添加处理下柠条粉发酵物的温度、理化性状、微生物种群数量、基质酶活性、发芽指数等指标的变化。结果表明:添加纤维素酶能够加快柠条粉发酵速度,柠条粉发酵60 d时,添加量为0.5 kg·m-3处理的发芽指数达到81.05%、添加量为1.25 kg·m-3处理的发芽指数达到83.45%;纤维素酶剂量增加能够提高发酵物电导率和柠条发酵物的养分释放量,增加速效氮质量分数;多酚氧化酶活性与纤维素酶使用剂量线性相关系数为0.903。当纤维素酶添加量为0.5 kg·m-3和1.25 kg·m-3时,柠条粉发酵60 d已达到腐熟及无毒害标准。可见,适当剂量纤维素酶的使用对柠条粉发酵物厌氧发酵具有促进作用,可明显加快柠条粉发酵速度,较之前发酵时间缩短1/3,建议使用0.5 kg·m-3纤维素酶制剂作为柠条粉发酵助剂用量。
In order to accelerate Caragana korshinskii powder fermentation and shorten fermentation time of Caragana korshinskii powder.Cellulose enzyme was used to accelerate fermentation of Caragana korshinskii powder,six treatments such as 0.125,0.25,0.5,1.25,2.5,0 kg·m-3 (CK) were done to observe temperature,physical and chemical properties,microbial populations,substrate enzyme activity,germination index and other indicators of change in Caragana korshinskii yeast powder under different doses of cellulase.The results showed that adding cellulose enzyme could speed up the fermentation of Caragana korshinskii powder,Caragana korshinskii powder yeast was 60 days,germination index of treatment reached 81.05% afer adding 0.5 kg·m-3 while adding 1.25 kg·m-3 germination index of treatment reached 83.45%;different doses of cellulose enzyme could improve the yeast conductivity,Caragana korshinskii yeast and nutrient release quantity, and increase the available nitrogen mass fraction;polyphenol oxidase activity and cellulose enzyme dosage for the linear correlation coefficient were 0.903.When cellulase amount was 0.5 kg·m-3 and 1.25 kg·m-3,powder yeast of Caragana korshinskii had reached 60 days rotten and specialties of the standard.So the appropriate dosage of cellulose enzyme could promote the anaerobic fermentation of Caragana korshinskii powder yeast,could also obviously accelerated the Caragana korshinskii powder fermentation,fermentation time was 1/3 shorter than reasonable use of Caragana korshinskii resources.We recommende to use cellulose enzyme preparation powder 0.5 kg·m-3 as agent dosage of Caragana korshinskii fermentation.

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