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-  2015 

不同活血化瘀方对扩张型心肌病小鼠心肌细胞的作用及对NF?拨?B通路的影响
Effects of different activating blood and removing stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and NF?拨?B pathway

Keywords: 活血化瘀,扩张型心肌病,NF?拨?B通路,小鼠
activating blood and removing blood stasis
,dilated cardiomyopathy,NF?拨?B pathway,mouse

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Abstract:

目的:研究不同活血化瘀方对扩张型心肌病小鼠心肌细胞的作用及对NF?拨?B通路的影响。方法:240只BALB/c小鼠随机分为方剂A组、方剂B组、模型组和空白组,每组60只。前3组采用柯萨奇B3m病毒制作实验室病毒性扩张型心肌病模型,方剂A组、方剂B组小鼠造模后分别以方剂A和方剂B灌胃治疗,模型组和空白组小鼠均灌注等量的纯水,1次/d,连续5周。比较各组小鼠的心脏质量指数。运用HE和VG染色法观察各组小鼠心肌细胞的病理学改变。采用TUNEL法检测小鼠心肌细胞的凋亡情况。采取RT??PCR法和免疫组化法检测各组小鼠心肌细胞NF?拨?B mRNA和蛋白的表达情况。结果:方剂A组、方剂B组和模型组小鼠心脏质量指数、心肌胶原容积分数、凋亡心肌细胞数及NF?拨?B mRNA和蛋白的表达均高于空白组(P<0.05);方剂A组和方剂B组小鼠的上述5个指标均低于模型组(P<0.05);方剂A组上述5个指标均低于方剂B组(P<0.05)。结论:不同活血化瘀方对扩张型心肌病小鼠心肌细胞的改善作用和对NF?拨?B通路的影响有差异。
Aim: To study the effect of different promoting blood circulation to remove blood stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and the effect on the NF?拨?B pathway.Methods: A total of 240 BALB/c mice were allocated into 4 groups with 60 in each group:group A,group B,model group,and control group.The model of dilated cardiomyopathy was made by B3m virus in the first 3 groups. The model mice in group A and group B were treated by two kinds of activating blood and removing blood stasis decoction. The heart mass index of the mice was compared. The pathological changes of myocardial cells from the mice were observed by HE and VG staining. The apoptosis of myocardial cells was detected by TUNEL. The expressions of NF?拨?B protein and mRNA were detected by immunohistochemical method and RT??PCR.Results: The heart mass index, myocardial collagen volume fraction, apoptosis of myocardial cells, and expressions of NF?拨?B protein and mRNA of group A, group B and the model group were higher than the control group(P<0.05). The 5 indexes of group A and group B were lower than those in the model group(P<0.05). And the 5 indexes of group A were lower than those in group B(P<0.05).Conclusion: The effects of different promoting blood circulation to remove blood stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and the effect on the NF?拨?B pathway are different

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