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香樟齿喙象成虫触角感器的微观特征分析

DOI: 10.3969/j.issn.1000-2006.201606018

Keywords: 香樟齿喙象, 扫描电镜, 触角, 感器, 超微结构
Pagiophloeus tsushimanus Morimoto
, scanning electron microscope, antennal, sensilla, ultrastructure

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Abstract:

【目的】香樟齿喙象(Pagiophloeus tsushimanus Morimoto)是新发现的危害香樟(Cinnamomum camphora)的钻蛀性害虫,研究触角感器超微形态有助于了解该害虫的嗅觉行为及其与寄主植物的相互关系。【方法】将成虫触角用超声波清洗并冷冻干燥,采用离子溅射仪喷金镀膜,再利用扫描电镜观察其外部形态、感器类型及分布状况。【结果】香樟齿喙象雌、雄成虫触角均呈膝状,共有7类11种感器类型,分别为3种刺形感器、2种毛形感器、2种锥形感器以及表皮孔、柱形感器、栓锥形感器和叉形感器。刺形感器是香樟齿喙象的主要感器类型,在性别之间除刺形感器Ⅰ型、Ⅱ型长度存在显著差异(P<0.05)外,其他类型感器长度、数量及分布均没有显著差异(P>0.05); 锥形感器和叉形感器仅在雌虫触角上发现。【结论】此次研究观察了香樟齿喙象成虫的触角感器类型,可为开发基于信息素的害虫防治方法提供理论依据。
【Objective】Pagiophloeus tsushimanus Morimoto is a wood-boring beetle that has recently been introduced to China causing serious damage to Cinnamomum camphora in Shanghai City and Fujian Province. Investigating the ultrastructure of the pests' antennal sensilla could elucidate its olfactory behaviors and interactions with its host.【Method】The antennae were submerged in an ultrasonic bath, freeze-dried and sputter-coated with gold. Then, the antennae morphology, the type, number and distribution of antennal sensilla were visualized by using scanning electron microscopy.【Result】The adult antennae were geniculate, seven types including 11 subtypes of sensilla were recorded: sensilla chaetica(Sch Ⅰ-Ⅲ), trichoid sensilla(St Ⅰ-Ⅱ), sensilla basiconica(Sb Ⅰ-Ⅱ), cuticular pore, cylindric sensilla, styloconica sensilla and sensilla furcatea. Sensilla chaetica represent the most abundant type of sensillum, and there were no significant sex-specific differences in length, number, or distribution of the antennal sensilla, except for the lengths of Sch Ⅰ and Sch Ⅱ. However, sensilla basiconica and sensilla furcatea were only found in females.【Conclusion】Studying on the ultrastructure of antennal sensilla of female and male P. tsushimanus provides a theoretical foundation for the development of pheromone-based P. tsushimanus control

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