The content and species of carotenoids are significantly affected by different carotenoids extraction methods. The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodopseudomonas faecalis PSB-B was carried out. Data ANOVA showed that ultrasound can greatly replace the conventional extraction. And then, based on ultrasonic assisting extraction method, the effect of ultrasonic time, solvent-solid ratio and ultrasonic power on the yield of carotenoids extracted from Rhodopseudomonas faecalis PSB-B was investigated using single factor and Box-Behnken experimental design. Under the extraction of temperature 20？C, N-hexane:Methanol (5:1), the optimal conditions for Ultrasonic assisted extraction of carotenoids found to be: Ultrasonic time 4.5 min, Solvent-solid ratio (mg/ml) (10:10), extraction power of 187 W. The yield of carotenoids could reach to 16.11 mg/L.
Mezzomo, N., Paz, E., Maraschin, M., et al. (2012) Supercritical Anti-Solvent Precipitation of Carotenoid Fraction from Pink Shrimp Residue: Effect of Operational Conditions on Encapsulation Efficiency. The Journal of Supercritical Fluids, 66, 342-349. https://doi.org/10.1016/j.supflu.2011.08.006
Michelon, M., de Borba, T.M., da Silva Rafael, R., et al. (2012) Extraction of Carotenoids from Phaffia rhodozyma: A Comparison between Different Techniques of Cell Disruption. Food Science and Biotechnology, 21, 1-8. https://doi.org/10.1007/s10068-012-0001-9
Farkade, V.D., Harrison, S. and Pandit, A.B. (2005) Heat Induced Translocation of Proteins and Enzymes within the Cell: An Effective Way to Optimize the Microbial Cell Disruption Process. Biochemical Engineering Journal, 23, 247-257. https://doi.org/10.1016/j.bej.2005.01.001
Lampila, L.E., Wallen, S.E., Bullerman, L.B., et al. (1985) The Effect of Illumination on Growth and β-Carotene Content of Blakeslea trispora Grown in Whey. Lebensmittel-Wissenschaft Technologie, 18, 370-373.
Sakaki, H., Nakanishi, T., Tada, A., et al. (2001) Activation of Torularhodin Production by Rhodotorula glutinis Using Weak White Light Irradiation. Journal of Bioscience and Bioengineering, 92, 294-297. https://doi.org/10.1016/S1389-1723(01)80265-6
Quackenbush, F.W. and Smallidge, R.L. (1986) Nonaqueous Reversedphase Liquid Chromatographic System for Separation and Quantitation of Pro-vitamins A. Journal of the Association of Official Analytical Chemists, 69, 767-772.
Sun, M. and Temelli, F. (2006) Supercritical Carbon Dioxide Extraction of Carotenoids from Carrot Using Canola Oil as a Continuous Co-Solvent. The Journal of Supercritical Fluids, 37, 397-408. https://doi.org/10.1016/j.supflu.2006.01.008
Zhang, H. and Hu, Q.P. (2015) Isolation, Identification and Physiological Characteristics of High Carotenoids Yield Rhodopseudomonas fecalis PSB-B. International Journal of Recent Scientific Research, 6, 3893-3899.
Khachik, F., Beecher, G.R. and Whittaker, N.F. (1986) Separation, Identification and Quantification of the Major Carotenoid and Chlorophyll Constituents in Extracts of Several Green Vegetables by Liquid Chromatography. Journal of Agricultural and Food Chemistry, 34, 603-616. https://doi.org/10.1021/jf00070a006
Charest, D.J., Bulaban, M.O., Marshall, M.R. and Cornell, J.A. (2001) Astaxanthin Extraction from Crawfish Shells by Supercritical CO2 with Ethanol as Co-Solvent. Journal of Aquatic Food Product Technology, 10, 79-93.
Kwon, J.H., Belanger, J.M.R., Pare, J.R., et al. (2003) Application of the Microwave-Assisted Process (MAP？) to the Fast Extraction of Ginseng Saponins. Food Research International, 36, 491-498. https://doi.org/10.1016/S0963-9969(02)00197-7
Tinoi, J., Rakariyatham, N. and Deming, R.L. (2005) Simplex Optimization of Carotenoid Production by Rhodotorula glutinis Using Hydrolyzed Mung Bean Waste Flour as Substrate. Process Biochemistry, 40, 2551-2557. https://doi.org/10.1016/j.procbio.2004.11.005