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Optimization Ultrasound Assisted Extraction of Carotenoids from Rhodopseudomonas faecalis PSB-B

DOI: 10.4236/oalib.1105045, PP. 1-10

Keywords: Rhodopseudomonas faecalis PSB-B, Carotenoids Extraction, Ultrasound Assisting

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Abstract:

The content and species of carotenoids are significantly affected by different carotenoids extraction methods. The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodopseudomonas faecalis PSB-B was carried out. Data ANOVA showed that ultrasound can greatly replace the conventional extraction. And then, based on ultrasonic assisting extraction method, the effect of ultrasonic time, solvent-solid ratio and ultrasonic power on the yield of carotenoids extracted from Rhodopseudomonas faecalis PSB-B was investigated using single factor and Box-Behnken experimental design. Under the extraction of temperature 20?C, N-hexane:Methanol (5:1), the optimal conditions for Ultrasonic assisted extraction of carotenoids found to be: Ultrasonic time 4.5 min, Solvent-solid ratio (mg/ml) (10:10), extraction power of 187 W. The yield of carotenoids could reach to 16.11 mg/L.

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