全部 标题 作者
关键词 摘要


Lipid Peroxidation and Antioxidant Enzymes Evaluation in Lactating Female Albino Rats Following Supplementation with Fermented Soya Bean and Vitamin C

DOI: 10.4236/aer.2017.52003, PP. 24-31

Keywords: Soya Bean, Lactation, Vitamin C, Lipid Peroxidation, Antioxidant Enzymes

Full-Text   Cite this paper   Add to My Lib

Abstract:

In mammals, lactation is the most energetically demanding period of a female’s reproductive life. This study was designed to evaluate the effect of fermented Soya bean and Vitamin C supplement on lipid peroxidation and antioxidant enzymes in lactating albino rats. Thirty five (35) adult female rats were used for this study. At parturition, the animals were randomly divided into five groups of five (5) rats each. Except group four (4) that was subdivided into three (3) sub groups of five animals each (n = 5). Treatment was carried out as follows: Group I: (Normal control) was given normal feed and distilled water, orally (1 ml/kg), Group II: metoclopramide (5 mg/kg), Group III: 100 mg/kg of Vitamin C. The three (3) sub groups under group four (4) received 10%, 20% and 40% soya bean, respectively, Group V: was co-administered with 20% soya bean supplement and Vitamin C (100 mg/kg). Treatment was done for the period of ten (10) days at 06:00 h daily. Although there was an increase in serum MDA concentrations in all the treated groups compared to the control, lipid peroxidation was however significantly higher (P < 0.05) in the metoclopramide group relative to the soya bean supplemented groups. This study has shown that supplementation with soya bean induces a mild antioxidant effect by increasing serum level of superoxide dismutase. There was however a significant decrease in serum SOD in the 10% SB group compared to the control. There was a significant difference in serum catalase activity in the group treated with METCL (46.20 ± 1.53), SB 10% (44.00 ± 1.14) and SB 20% (45.20 ± 1.28) compared to the control (52.00 ± 0.71) (P < 0.05). Serum level of glutathione peroxidase GPx showed a significant difference in the group treated with VIT C, SB 10% and SB 20% compared to the control (P < 0.05).

References

[1]  Felipe, P.T., Juleana, V.B.J. and Zulma T.R. (2014) Pharmacological Overview of Galactogogues. Veterinary Medicine International, 66, 505-511.
[2]  Halliwell, B. and Gutteridge, J.M.C. (1998) The Antioxidants of Human Extracellular Fluids. Archives of Biocmistry and Biophysics Impact Factor, 280-218.
[3]  Castillo, C., Hernández, J. and Valverde, I. (2006) Plasma Malonaldehyde (MDA) and Total Antioxidant Status (TAS) during Lactation in Dairy Cows. Research in Veterinary Science, 80, 133-139.
https://doi.org/10.1016/j.rvsc.2005.06.003
[4]  Upreti, K., Chaki, S.P. and Misro, M.M. (2002) Evaluation of Peroxidative Stress and Enzymatic Antioxidant Activity in Liver and Kidney during Pregnancy and Lactation in Rats. Health Population Perspective Issues, 25, 177-185.
[5]  Kim, G.Y., Moon, H.K., Lee, S.W., Moon, J.N. and Yoon, W.J. (2010) Effect of Mixed Soybeans Materials on Quality Characteristics of Traditional Soybean Paste (Doenjang) during Aging. Korean Journal of Food and Cookery Science, 26, 314-322.
[6]  Yoo, K.M., Hwang, J.Y. and Lee, S.M. (2011) Development of Soybean Snacks and Analysis of Bioactive Compounds. Korean Journal of Food and Nutrition, 24, 702-707.
https://doi.org/10.9799/ksfan.2011.24.4.702
[7]  Marthins, M.I. (2006) Zaria and Its Regions. Ahmadu Bello University, Zaria, 41.
[8]  Janero, D.R. (1990) Malondialdehyde and Thiobarbituric Acid-Reactivity as Diagnostic Indices of Lipid Peroxidation and Peroxidative Tissue Injury. Free Radical Biology and Medicine, 9, 515-540.
https://doi.org/10.1016/0891-5849(90)90131-2
[9]  Martin, J.P., Daily, M. and Sugarrnan, E. (1987) Negetive and Positive Assay of Superoxide Dismutase Based on Hematoxylin Autoxidation. Archives of Biochemistry and Biophysics, 255, 329-336.
https://doi.org/10.1016/0003-9861(87)90400-0
[10]  Beers, R.F. and Sizer, I.W. (1952) A Spectrophotometric Method for Measuring the Breakdown of Hydrogen Peroxide by Catalase. Journal of Biological Chemistry, 195, 133-140.
[11]  Avissar, N., Eisenmann, C. and Breen, J.G. (1994) Human Placenta Makes Extracellular Glutathione Peroxidase Secretes It into Maternal Circulation. American Journal of Physiology, 267, 68-76.

Full-Text

comments powered by Disqus