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磷酸盐对盐生杜氏藻生长和细胞色素积累的影响
Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina

DOI: 10.12677/AMS.2016.33014, PP. 100-107

Keywords: KH2PO4/sub>,盐生杜氏藻,生长,β-胡萝卜素,吸收规律
KH2PO4/sub>
, Dunaliella salina, Growth, β-Carotene, Absorpted Patterns

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Abstract:

研究了不同浓度(0~0.30 mmol/L)的KH2PO4对盐生杜氏藻生长和细胞色素积累的影响,结果表明,添加0.10 mmol/L KH2PO4,细胞生长最快,最高密度为153 × 104 cell/mL,无磷对照组密度最低,仅为36 × 104 cell/mL;KH2PO4浓度为0.20 mmol/L时,β-胡萝卜素积累量最高,为12.8 mg/L,其次是0.15 mmol/L,对照组很低,仅为6.28 mg/L,KH2PO4超过0.25 mmol/L对β-胡萝卜素积累有抑制作用;适当添加KH2PO4对D. salina叶绿素a合成有促进作用;在培养的前6天,pH值明显上升,随后4~5天又下降;添加0.1 mmol/L KH2PO4细胞蛋白质含量最高,为37.34%,对照组仅为24.86%;0.10 mmol/L组的KH2PO4在6天内吸收完毕,KH2PO4吸收的时间与其最初的浓度呈正相关关系,建立了藻液中KH2PO4被盐生杜氏藻吸收的动力学方程。
The effects of KH
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