Para-dichlorobenzene (p-DCB) is widely used as a chemical intermediate in manufacturing of dyes, pharmaceuticals, polymers and other organic synthesis. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal, and spectroscopic properties of p-dichlorobenzene. The p-dichlorobenzene sample was divided into two groups that served as treated and control. The treated group received Mr. Trivedi’s biofield treatment. Subsequently the control and treated samples were evaluated using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and UV-Vis spectroscopy. XRD result showed an increase in crystallite size (4.93%) along with alteration in peak intensity of treated sample as compared to control. Furthermore, DSC analysis results showed that the latent heat of fusion of treated p-dichlorobenzene was considerably reduced by 8.66% as compared to control. The reduction in melting point of treated sample (54.99°C) was also observed as compared to control (57.01°C) p-dichlorobenzene. Moreover, TGA/DTG studies showed that Tmax (temperature, at which sample lost maximum of its weight) was increased by 6.26% and weight loss per degree celsius (°C) was decreased by 12.77% in biofield treated p-dichlorobenzene as compared to control sample. It indicates that thermal stability of treated p-dichlorobenzene sample might increase as compared to control sample. However, no change was found in UV-Vis spectroscopic character of treated p-dichlorobenzene as compared to control. These findings suggest that biofield treatment has significantly altered the physical and thermal properties of p-dichlorobenzene, which could make it more useful as a chemical intermediate.