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多柔比星对体外培养小鼠腔前卵泡发育的影响

Keywords: ?多柔比星v腔前卵泡,生殖毒性,卵丘-卵母细胞复合体

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Abstract:

?目的:利用雌性icr小鼠腔前卵泡体外培养方法观察多柔比星对卵泡发育的影响,对多柔比星卵巢毒性机制进行初步探讨。方法:取12~14日龄的雌性icr小鼠卵巢的腔前卵泡进行原代培养,在培养的第2、6和11天,分别用不同浓度的多柔比星(0.4、0.8、1.6和3.2μg/ml)染毒24h后继续培养。通过测量卵泡的大小、存活率和卵丘-卵母细胞复合体(cumulus-oocytecellcomplexes,cocs)的排出率来判定多柔比星对腔前卵泡发育的影响。结果:在实验所设置的剂量范围内,与对照组比较,多柔比星在1.6和3.2μg/ml浓度下能明显抑制雌性icr小鼠卵巢腔前卵泡的发育(p<0.05),降低卵泡的存活率(p<0.05),并抑制cocs的排出(p<0.05),且呈一定的剂量效应关系。结论:多柔比星可抑制腔前卵泡的发育,降低卵泡的存活率,抑制cocs的排出,从而引起卵巢毒性,产生卵巢功能障碍。

References

[1]  knobfmt.theinfluenceofendocrineeffectsofadjuvanttherapyonqualityoflifeoutcomesinyoungerbreastcancersurvivors[j].oncologist,2006,11(2):96-110.
[2]  chemaitillyw,mertensac,mitbyp,etal.acuteovarianfailureinthechildhoodcancersurvivorstudy[j].jclinendocrinolmetab,2006,91(5):1723-1728.
[3]  leesj,schoverlr,partridgeah,etal.americansocietyofclinicaloncologyrecommendationsonfertilitypreservationincancerpatients[j].jclinoncol,2006,24(18):2917-2931.
[4]  ben-aharoni,bar-josephh,tzarfatyg,etal.doxorubicin-inducedovariantoxicity[j].reprodbiolendocrinol,2010,8:20.
[5]  rotiec,leismansk,abbottdh,etal.acutedoxorubicininsultinthemouseovaryiscell-andfollicle-typedependent[j].plosone,2012,7(8):42293.
[6]  sunf,betzendahli,sheny,etal.preantralfolliclecultureasanovelinvitroassayinreproductivetoxicologytestinginmammalianoocytes[j].mutagenesis,2004,19(1):13-25.
[7]  万旭英,朱玉平,马玺里,等.小鼠腔前卵泡体外培养方法的建立[j].卫生研究,2009,38(2):153-158.
[8]  梅树江,林晓潭,王晓梅,等.小鼠腔前卵泡体外培养成熟染色体分析技术的建立[j].癌变·畸变·突变,2009,21(6):463-466.
[9]  刘晓,邵方元,陈宏远.阿霉素抗肿瘤分子机制的研究进展[j].中国医药生物技术,2012,7(5):373-375.
[10]  cecconis,ciccarellic,barberim,etal.granulosacelloocyteinteractions[j].biolreprod,2004,115(sup1):19-22.

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