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连续与间断西地那非干预对糖尿病大鼠阴茎海绵体影响的实验研究

Keywords: 阴茎勃起功能障碍,糖尿病,枸橼酸西地那非,大鼠

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Abstract:

目的观察并比较连续及间断西地那非(SDF)干预对糖尿病大鼠阴茎勃起功能及组织病变影响的异同。方法雄性SD大鼠70只,60只腹腔注射STZ诱发糖尿病,10只注射生理盐水作正常对照。成功诱发糖尿病大鼠53只,分为糖尿病组(DM组,25只)、SDF连续治疗组(SDL组,14只),SDF间断治疗组(SDS组,14只)。SDL组大鼠给予SDF2mg?(kg?d)-1灌胃,连续给药12周,SDS组大鼠在阿朴吗啡(APO)诱导阴茎勃起实验前给予SDF20mg?(kg?d)-1灌胃,连续给药3d。分别在糖尿病造模成功后的第4、8、12周进行APO诱导阴茎勃起试验,12周时光镜、透射电镜下观察各组大鼠阴茎组织病变情况。结果DM组阴茎勃起功能随病程延长进行性下降,与正常对照组相比勃起率及勃起次数差异均有统计学意义(P0.05)。DM组阴茎组织光镜、电镜下病理损坏明显,与之相比SDL组损伤程度更为严重,SDS组较DM组无明显变化。结论连续及间断SDF干预对早期糖尿病大鼠勃起功能均有显著改善作用,但随病程延长此作用明显下降,且连续SDF干预的改善作用消减更为明显。间断SDF干预对糖尿病大鼠阴茎组织病理改变无明显影响,而长期连续SDF干预则加重糖尿病大鼠阴茎海绵体病理损害程度。

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