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水体铜对黄河鲤肝胰脏抗氧化酶活性和总抗氧化能力的影响

Keywords: ,黄河鲤,肝胰脏,抗氧化酶,总抗氧化能力

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Abstract:

为了研究不同浓度的水体铜对黄河鲤肝胰脏抗氧化能力的影响,采用0.01、0.05、0.10、0.30、0.50、0.70和1.00mg·L-1Cu2+分别刺激黄河鲤1、3、5、7d后,测定黄河鲤肝胰脏抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT和谷胱甘肽过氧化物酶GPx)的活性及总抗氧化能力(T-AOC)。结果表明,黄河鲤暴露于0.01mg·L-1Cu2+溶液时,SOD、CAT和GPx活性及T-AOC高于对照组,其中GPx活性达到最大,且显著高于对照组(P<0.05),T-AOC3、5、7d时,显著高于对照组(P<0.05);Cu2+浓度为0.05mg·L-1时,SOD和CAT活性及T-AOC(P<0.05)达到最大,其中SOD和CAT活性3、5、7d时,显著高于对照组(P<0.05),GPx活性1d时高于对照组,3、5、7 d时低于对照组,其中5、7d时,显著低于对照组(P<0.05);Cu2+浓度为0.10mg·L-1时,SOD活性3、5、7d显著低于对照组(P<0.05),CAT和GPx活性及T-AOC显著低于对照组(P<0.05);高浓度组(0.30、0.50、0.70和1.00mg·L-1)Cu2+抑制了SOD、CAT和GPx活性及T-AOC。结果提示,黄河鲤肝胰脏SOD、CAT和GPx活性及T-AOC对铜的污染均具指示作用,其中最为灵敏的是GPx活性,可以用来指示低剂量重金属的污染。

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