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饲粮中不同锌源及锌水平对固始鸡和AA肉鸡肝脏金属硫蛋白含量及基因表达的影响

DOI: 10.7685/j.issn.1000-2030.2012.06.018, PP. 111-117

Keywords: ,固始鸡,AA肉鸡,金属硫蛋白(MT),MTmRNA

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Abstract:

为了研究肉鸡肝脏中金属硫蛋白(metallothionein,MT)含量和MTmRNA表达水平作为评定锌源生物利用率的有效性,在固始鸡和AA肉鸡饲料中添加不同水平的饲料级硫酸锌(ZnSO4?H2O)、氧化锌(ZnO)和氨基酸络合锌(ZnAA),分别测定了2、3、4、5和6周龄末肝脏中MT含量和MTmRNA的表达水平。结果表明品种间肝脏中MT含量和MTmRNA表达水平差异极显著,固始鸡高于AA肉鸡。锌源和锌添加水平对所测定指标产生显著影响,氨基酸络合锌显著高于硫酸锌,这两种锌源极显著高于氧化锌;随着锌添加水平增加,肉鸡肝脏中MT含量和MTmRNA表达水平提高,锌水平之间差异极显著。4周龄前,随着周龄增长,肝脏中MT含量和MTmRNA水平显著升高,而4周龄后随周龄显著降低。以肝脏中MT含量评定的锌源间相对生物利用率与以MTmRNA表达水平为指标评定的结果一致性较好,说明肝脏中MT含量和MTmRNA表达水平是评定锌源相对生物利用率的有效指标,相比较而言,MT含量受品种、周龄和锌添加水平的影响大于MTmRNA表达水平。

References

[1]  Ohki K.Zinc nutrition related to critical deficiency and toxicity levels for sorghum[J].Agronomy Journal,1984,76:253-256
[2]  Richards M P,Cousins R J.Metallothionein and its relationship to the metabolism of dietary zinc in rats[J].Journal of Nutrition,1976,106(11):1591-1599
[3]  Aydemir T B,Blanchard R K,Cousins R J.Zinc supplementation of young men alters metallothionein,zinc transporter,and cytokine gene expression in leukocyte populations[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(6):1699-1704
[4]  Fleet J C,Qureshi M A,Dietert R R,et al.Tissue-specific accumulation of metallothionein in chickens as influenced by the route of zinc administration[J].Journal of Nutrition,1988,118(2):176-182
[5]  曹家银,罗绪刚,Davis S R,等.以组织锌、金属硫蛋白及其基因表达指标评价肉仔鸡对锌源的相对生物学利用率[J].畜牧兽医学报,2003,34(3):227-231
[6]  黄艳玲,吕林,罗绪刚,等.饲粮锌水平对肉仔鸡组织锌转运蛋白基因表达的影响[J].营养学报,2008,30(5):475-479
[7]  Mohanna C,Nys Y.Infuence of age,sex and cross on body concentrationsof trace elements(zinc,iron,copper and manganese)in chickens[J].British Poultry Science,1998,39:536-543
[8]  高延玲.固始鸡微量元素锰、锌需要量的研究.郑州:河南农业大学,2004
[9]  Wedekind K J,Hortin A E,Baker D H.Methodology for assessing zinc bioavailability:efficacy estimates for zinc-methionine,zinc sulfate,and zinc oxide[J].Journal of Animal Science,1992,70(1):178-187
[10]  Sandoval M,Henry P R,Ammerman C B,et al.Relative bioavailability of supplemental inorganic zinc sources for chicks[J].Journal of Animal Science,1997,75:3195-3205
[11]  Richards M P,Cousins R J.Mammalian zinc homeostasis:requirement for RNA and metallothionein synthesis[J].Biochem Biophys Res Commun,1975,64(4):1215-1223
[12]  Blanchard R K,Cousins R J.Differential display of intestinal mRNAs regulated by dietary zinc[J].Proceedings of the National Academy of Sciences of the United States of America,1996,93(14):6863-6868
[13]  Liuzzi J P,Blanchard R K,Cousins R J.Differential regulation of zinc transporter 1,2,and 4 mRNA expression by dietary zinc in rats[J].Journal of Nutrition,2001,131(1):46-52
[14]  Bittel D,Dalton T,Samson S L,et al.The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc,but not by other transition metals[J].Journal of Biological Chemistry,1998,273(12):7127-7133
[15]  Coppenet M,Golven J,Simon J C,et al.Evolution chimique des sols en exploitations d’élevage intensif:exemple du Finistère[J].Agronomie,1993,13:77-83
[16]  Giordano P M,Mortvedt J J,Mays D A.Effect of municipal wastes on crop yields and uptake of heavy metals[J].Journal of Environmental Quality,1975,4(3):394-399
[17]  Emmert J L,Baker D H.Zinc stores in chickens delays the onset of zinc deficiency symptoms[J].Poultry Science,1995,74:1011-1021
[18]  Eaton D L,Toal B F.Evaluation of the Cd/Hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues[J].Toxicology and Applied Pharmacology,1982,66:134-142
[19]  Gittins J E,Overfield N D.Quality of Poultry ProductsⅡ.Eggs and Egg Products[M].Beekbergen,Spelderholt:Centre for Poultry Research and Information Services,1991:291
[20]  Hahn J D,Baker D H.Growth and plasma zinc responses of young pigs fed pharmacologic levels of zinc[J].Journal of Animal Science,1993,71(11):3020-3024
[21]  Kidd M T,Ferket P R,Qureshi M A.Zinc metabolism with special reference to its role in immunity[J].World’s Poultry Science Journal,1996,52(3):309-324
[22]  Udom A O,Brady F O.Reactivation in vitro of zinc-requiring apo-enzymes by rat liver zinc-thionein[J].Biochem J,1980,187:329-335
[23]  Blalock T L,Dunn M A,Cousins R J.Metallothionein gene expression in rats:tissue-specific regulation by dietary copper and zinc[J].Journal of Nutrition,1988,118(2):222-228
[24]  Huang Y L,Lu L,Li S F,et al.Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet[J].Journal of Animal Science,2009,87:2038-2046
[25]  Cao J,Henry P R,Guo R,et al.Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants[J].Journal of Animal Science,2000,78:2039-2054
[26]  Sandoval M,Henry P R,Luo Y X,et al.Performance and tissue zinc and metallothionein accumulation in chicks fed a high dietary level of zinc[J].Poultry Science,1998,77:1354-1363

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