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50Hz0.1mT三角波电磁场提高大鼠峰值骨量

, PP. 2349-2352

Keywords: 三角波电磁场,骨密度,峰值骨量

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Abstract:

目的研究50Hz、0.1mT三角波电磁场对大鼠峰值骨量(peakbonemass,PBM)的影响。方法将5周龄的雌性SD大鼠分成对照组和实验组(n=9)。实验组每天使用三角波电磁场处理90min,对照组除不进行磁场干预外其他条件与实验组相同。在磁场处理中的第1、2、3个月末,腹腔麻醉测量骨密度(bonemineraldensity,BMD)。第3个月末心脏取血处死。ELISA试剂盒检测血清中碱性磷酸酶(bonealkalinephosphatase,BALP)、瘦素(leptin,LEP)和抗酒石酸酸性磷酸酶(tartrateresistantacidphosphatase5b,Tracp5b)含量,并进行骨形态计量分析。?结果?实验组在1、2个月末,BMD检测与对照组相比无显著性差异(P>0.05);3个月末,实验组BMD检测与对照组相比有显著性差异(P<0.05)。血清生化检测结果显示实验组ALP含量上升,Tracp5b和LEP含量下降,与对照组相比具有显著性差异(P<0.05)。实验组双荧光间距离和骨小梁宽度均高于对照组,有显著性差异(P<0.05)。结论低频三角波电磁场能够提高大鼠PBM。

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