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核农学报  2015 

杜仲内生真菌DZJ07在小麦根际定殖及对根部酶活的影响

DOI: 10.11869/j.issn.100-8551.2015.06.1149, PP. 1149-1157

Keywords: 杜仲,内生真菌,定殖,小麦根际,小麦纹枯病,酶活性

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Abstract:

为了研究生防菌在植物根际定殖中的作用,本研究以一株对小麦纹枯病菌有较好抑菌活性的杜仲内生真菌DZJ07为试验菌株,通过浓度梯度法对其进行抗性标记,分析其在小麦根际的定殖特点;同时通过盆栽接种试验研究菌株抗性突变株DZJ07-2在小麦根际定殖对不同生育期的小麦植株根部中POD、PPO和PAL活性的影响。结果表明:筛选出的突变株菌株DZJ07-2能够耐受浓度为200μg·mL-1的80%多菌灵,具有较好的遗传稳定性;该突变株能成功在小麦根际定殖,接种第20天小麦根际土壤中DZJ07-2菌株数量达到最大值6.33×102cfu·g-1;菌株DZJ07-2在小麦根际的定殖能诱导小麦植株根部的PPO、POD和PAL3种防御酶活性的提高,在一定程度上增强了小麦对纹枯病的抗性,其苗期小麦纹枯病的发病率仅为13.36%,明显低于未经DZJ07-2处理的小麦纹枯病的发病率(90.25%)。本研究为揭示其生防机理及应用该菌提供了科学依据。

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