All Title Author
Keywords Abstract

核农学报  2015 

铅胁迫下萝卜基因组DNA甲基化分析

DOI: 10.11869/j.issn.100-8551.2015.07.1278, PP. 1278-1284

Keywords: 萝卜,铅胁迫,DNA甲基化,甲基化敏感扩增多态性

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用甲基化敏感扩增多态性(MSAP)技术,分析不同浓度铅(Pb)处理下萝卜基因组DNA甲基化水平的变化,探讨DNA甲基化在植株对Pb胁迫反应中的作用。结果表明,经100、400和800mg·L-1Pb(NO3)2处理后,MSAP比率分别为12.7%、14.2%和17.0%,均高于对照;全甲基化率分别为9.7%、10.2%和12.8%,而其对照为9.5%,表明重金属铅胁迫后,某些位点发生了重新甲基化。萝卜肉质根中总甲基化水平随Pb处理浓度上升而提高。甲基化变异可分为重新甲基化、去甲基化、不定类型以及与对照相同等甲基化类型。萝卜Pb胁迫处理引起的植株基因组甲基化程度的提高主要是重新甲基化。研究为从DNA水平揭示重金属Pb毒害机理及植物对Pb胁迫的响应机制提供了理论依据。

References

[1]  Wadhwa S K, Tuzen M, Kazi T G, Soylakc M, Hazerd B. Polyhydroxybutyrate-b-polyethyleneglycol block copolymer for the solid phase extraction of lead and copper in water, baby foods, tea and coffee samples [J]. Food Chemistry, 2014, 152: 75-80
[2]  陈璨, 常云霞, 古红梅, 李俐俐, 陈龙. 重金属及有机物复合污染对大豆生理特性和 DNA 损伤效应的影响[J]. 大豆科学, 2012, 31(6): 915-919
[3]  Black M C, Ferrel J R, Horning R C. DNA strand breakage in fresh water Mussels (Anodonta grandis) exposed to lead in the laboratory and field [J]. Environmental Toxicology and Chemistry, 1996, 15(5): 802-808
[4]  肖志华, 张义贤, 张喜文, 李萍. 外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响[J]. 生态学报, 2012, 32(3): 889-897
[5]  Peng H, Zhang J. Plant genomic DNA methylation in response to stresses: potential applications and challenges in plant breeding [J]. Progress in Natural Science, 2009, 19(9): 1037-1045
[6]  Ou X F, Zhang Y H, Xu C M, Lin X Y, Zang Q, Zhuang T T, Jiang L L, von Wettstein D, Liu B. Transgenerational inheritance of modified DNA methylation patterns and enhanced tolerance induced by heavy metal stress in rice (Oryza sativa L.) [J]. PLoS One, 2012, 7(9): e41143
[7]  孟华兵, 杜雪, 姜宇晓, 朴学成, 郭万里, 蒋立希. 镉胁迫下二倍体和同源四倍体油菜 DNA 甲基化差异分析[J]. 核农学报, 2010, 24(6): 1297-1304
[8]  葛才林, 杨小勇, 刘向农, 孙锦荷, 罗时石, 王泽港. 重金属对水稻和小麦DNA甲基化水平的影响[J]. 植物生理与分子生物学学报, 2002, 28(5): 363-368
[9]  黑淑梅. 重金属铬对小麦根系 DNA 甲基化水平的影响[J]. 吉林农业科学, 2012, 37(2): 14-15
[10]  Labra M, Grassi F, Imazio S, Di Fabioc T, Citterioa S, Sgorbatia S, Agradic E. Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L[J]. Chemosphere, 2004, 54(8): 1049-1058
[11]  Bender J. Cytosine methylation of repeated sequences in eukaryotes: the role of DNA pairing[J]. Trends in Biochemical Sciences, 1998, 23(7): 252-256
[12]  Aina R, Sgorbati S, Santagostino A, Labra1 M, Ghiani A, Citterio S. Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp [J]. Physiologia Plantarum, 2004, 121(3): 472-480
[13]  杨美娜, 杨瑰丽, 郭涛, 刘永柱, 张建国, 陈志强, 王慧. 逆境胁迫下植物DNA甲基化及其在抗旱育种中的研究进展[J]. 中国农学通报, 2013, 29(6):6-11
[14]  Long L, Lin X, Zhai J, Kou H, Yang W, Liu B. Heritable alteration in DNA methylation pattern occurred specifically at mobile elements in rice plants following hydrostatic pressurization [J]. Biochemical and Biophysical Research Communications, 2006, 340(2): 369-376
[15]  Causevic A, Delaunay A, Ounnar S, Righezza M. DNA methylating and demethylating treatments modify phenotype and cell wall differentiation state in sugarbeet cell lines [J]. Plant Physiology and Biochemistry, 2005, 43(7): 681-691
[16]  Guimil S, Dunand C. Patterning of Arabidopsis epidermal cells: Epigenetic factors regulate the complex epidermal cell fate pathway [J]. Trends in Plant Science, 2006, 11(12): 601-609
[17]  李照令, 王鹤潼, 陈瑞娟, 贾春云, 李晓军, 台培东, 李培军, 刘宛. 运用MSAP 研究镉胁迫对拟南芥幼苗基因甲基化的影响[J].农业环境科学学报, 2014, 33(1): 28-36
[18]  Zilberman D. The evolving functions of DNA methylation [J]. Current Opinion in Plant Biology, 2008, 11(5): 554-559
[19]  韩雅楠, 赵秀娟, 蔡禄. MSAP 技术在植物抗逆性方面的应用[J]. 生物技术通报, 2010, (6): 71-74
[20]  Richards E J. DNA methylation and plant development [J]. Trends in Genetics, 1997, 13(8): 319-323
[21]  Holliday R. The inheritance of epigenetic defects [J]. Science, 1987, 238(4824): 163-170
[22]  王子成, 李忠爱, 李锁平. MSAP技术及其在植物上的应用[J]. 生物技术通讯, 2006(S1): 195-196
[23]  Portis E, Acquadro A, Comino C, Lanteri S. Analysis of DNA methylation during germination of pepper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP) [J]. Plant Science, 2004, 166(1): 169-178
[24]  杨岚, 李红, 向增旭. 二倍体和同源四倍体甜叶菊的 DNA 甲基化差异分析[J]. 核农学报, 2013, 27(8): 1125-1130
[25]  徐小万, 雷建军, 张长远, 李颖, 王恒明, 李涛, 徐晓美. 高温多湿胁迫下辣椒 DNA 甲基化分析[J]. 核农学报, 2014, 28(7):1175-1180
[26]  汪隆植, 何启伟. 中国萝卜[M]. 北京: 科学技术文献出版社, 2005
[27]  Goyeneche R, Di Scala K, Roura S. Biochemical characterization and thermal inactivation of polyphenol oxidase from radish (Raphanus sativus var. sativus) [J]. LWT-Food Science and Technology, 2013, 54(1): 57-62
[28]  Liu L, Guo W, Zhu X, Zhang T. Inheritance and fine mapping of fertility-restoration for cytoplasmic male sterility in Gossypium hirsutum L [J]. Theoretical and Applied Genetics, 2003, 106(3): 461-469
[29]  周新文, 朱国念, Mwalilino J, 孙锦荷. Cu、Zn、Pb、Cd 及其混合重金属离子对鲫鱼DNA 甲基化水平的影响[J]. 中国环境科学, 2001, 21(6): 549-552
[30]  Dahl C, Guldberg P. DNA methylation analysis techniques [J]. Biogerontology, 2003, 4(4): 233-250
[31]  Xiong L Z, Xu C G, Saghai-Maroof M A, Zhang Q. Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique [J]. Molecular and General Genetics, 1999, 261(3): 439-446
[32]  Adam R L P, Burdon R H. Molecular Biology of DNA Methylation [M]. New York: Springer-verlag, 1985
[33]  聂丽娟, 王子成. DNA 甲基化抑制剂作用机理及其在植物发育生物学研究中的应用[J]. 核农学报, 2007, 21(4): 362-365
[34]  梁喜龙, 方淑梅, 胡瑞学. 植物 DNA 甲基化与逆境响应[J]. 中国生物制品学杂志, 2012, 25(8): 1064-1068
[35]  朱国念, 周新文, 孙锦荷. 混合重金属离子诱导的鲫鱼 DNA 损伤与基因表达[J]. 核农学报, 2003, 17(3): 199-202
[36]  葛才林, 杨小勇, 刘向农, 孙锦荷, 罗时石, 王泽港. 重金属胁迫对作物DNA胞嘧啶甲基化的影响[J]. 农业环境保护, 2002, 21(4): 301-305
[37]  王丙莲, 张迎梅, 谭玉凤, 候亚妮, 刘阳. 镉铅对泥鳅DNA甲基化水平的影响[J]. 毒理学杂志, 2006, 20(2): 78-80
[38]  杨金兰, 柳李旺, 龚义勤, 黄丹琼, 王峰, 何玲莉. 镉胁迫下萝卜基因组DNA甲基化敏感扩增多态性(MSAP)分析[J]. 植物生理与分子生物学学报, 2007, 33(3): 219-226
[39]  王子成, 马洪霞, 何艳霞. 重金属镉对拟南芥DNA 甲基化的影响[J].植物生理学通讯, 2009, 45(2): 115-118
[40]  马浪浪, 江舟, 黄小波, 沈亚欧, 潘光堂. 植物DNA甲基化调控研究进展[J]. 中国生物工程杂志, 2013, 33(9):101-110
[41]  Hix S, Augusto O. DNA methylation by tert-butyl bydroperoxide-iron (Ⅱ): a role for the transition metal ion in production of DNA base adducts [J]. Chemico-Biological Interactions, 1999, 118(2): 141-149
[42]  黑淑梅. 重金属 Cr 6+ 胁迫下小麦幼苗 DNA 5-MeC 含量的改变[J]. 广西植物, 2012, 32(6): 788-792
[43]  Sciandrello G, Caradonna F, Mauro M, Barbata G. Arsenic-induced DNA hypomethylation affects chromosoma instability in mammalian cells [J]. Carcinogenesis, 2004, 25(3): 413-417
[44]  Baccarelli A, Bollati V. Epigenetics and environmental chemicals [J]. Current Opinion in Pediatrics, 2009, 21(2): 243-251
[45]  孙颖, 葛锋, 刘迪秋, 陈朝银. 植物中DNA甲基化模式及其相关机制[J]. 植物生理学报, 2011, 47(8): 745-751
[46]  He L L, Liu L W, Gong Y Q, Hou X L, Zhu X W, Yang J L, Wu B. Physiological responses of radish (Raphanus sativus L.) to lead stress [C]//IEEE 2008. The 2nd International Conference on Bioinformatics and Biomedical Engineering 2008. Shanghai, 2008: 4602-4605

Full-Text

comments powered by Disqus