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美洲大蠊药材HPLC指纹图谱研究
Fingerprint of Periplaneta americana by HPLC

DOI: 10.12677/AAC.2015.53005, PP. 39-48

Keywords: 美洲大蠊药材,特征指纹图谱,HPLC
Periplaneta americana
, Characteristic Chromatographic Profile, HPLC

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Abstract:

采用Diamonsil-C18(1)色谱柱(250 mm × 4.6 mm, 5 μm),流动相乙腈-0.1%三氟乙酸水溶液,检测波长256 nm,结果10批不同来源的美洲大蠊药材标定了22个共有峰,其中指认了3个共有峰,其相似度大于0.950;与美洲大蠊药材对照指纹图谱比较,1批黑胸大蠊药材的相似度为0.990,但只有18个共有峰。本文建立的方法具有简便,重复性好,特征性强的特点,可用于美洲大蠊药材的鉴别与质量评价。
Taking Diamonsil C18(1) column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% trifluoroacetic acid as mobile phase, detection wavelength 256 nm, we got a result that there were 22 common peaks in batches of Periplaneta Americana, the peaks similarity was more than 0.950, three com-mon peaks were identified, the similarity of Periplaneta fulginosa was 0.990 by comparing with the control fingerprint, but there were 18 common peaks. The method is repeatable and feasible and can be suitable for the quality control of Periplaneta americana.

References

[1]  清?黄奭辑 (1982) 神农本草经. 中医古籍出版社, 北京, 238.
[2]  国家中医药管理局《中华本草》编委会 (1999) 中华本草. 上海科学技术出版社, 上海, 149-151.
[3]  云南省食品药品监督管理局编 (2006) 云南省中药材标准(2005年版). 云南美术出版社, 昆明, 246.
[4]  吴红梅, 李晨晨, 刘楠, 等 (2012) 闪式与回流提取美洲大蠊总氨基酸的工艺比较. 中国实验方剂学杂志, 21, 52-53.
[5]  杨芳, 邵金良, 汪庆平, 等 (2010) 美洲大蠊水解氨基酸含量测定及营养评价. 氨基酸和生物资源, 1, 9-11.
[6]  肖培云, 杨永寿, 李树楠 (2012) 不同养殖场美洲大蠊中甘露醇的含量测定. 中国现代应用药学, 2, 156-158.
[7]  郭念欣, 李颖春, 谢伟桥, 等 (2011) 广陈皮与陈皮HPLC指纹图谱的建立与鉴别. 中国实验方剂学杂志, 7, 90-93.
[8]  王祥培, 黄婕, 靳凤云, 等 (2010) 威灵仙类药材HPLC指纹图谱鉴别研究. 中成药, 5, 704-708.
[9]  刘璃曦, 王荣荣, 齐文, 等 (2013) 基于 HPLC 特征指纹图谱的蒲黄炭的鉴别研究. 中成药, 1, 127-131.
[10]  李辰, 刘志平, 邸多隆 (2013) 快速鉴别原药材与其胶囊制剂的HPLC指纹图谱比对法. 中成药, 1, 143-147.
[11]  王利春, 孔繁贵, 瘳军, 等 (2008) 一种美洲大蠊药材及其提取物的指纹图谱质量测定方法. 中国, ZL200810116773.0.
[12]  万德光 (1997) 中药分类学. 人民卫生出版社, 北京, 155-193.
[13]  焦春香, 张成桂, 刘光明, 等 (2011) 心脉隆注射液HPLC指纹图谱建立方法的研究. 中成药, 10, 1648-1652.

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