Effectiveness of Apricots (Prunus armeniaca), Pomegranate (Punica granatum) Juice and Lactic Acid Fermented Sobya on Plasma Levels of Lipid Profile Parameters and Total Homocysteine among Egyptian Adults
Objectives: The aim of this study was to investigate the effectiveness of dietary intervention with apricots, pomegranate juice or lactic acid fermented sobya on improving the plasma levels of lipid profile parameters and total plasma homocysteine (tHcy) among healthy adults. Design of the study: Thirty-five men and women (28.9 ± 3.0 years) with mean body mass index of 23.5 ± 1.2 kg/m2 were randomly assigned to a dietary intervention study. During the pre-feeding study records of 24-hour food intake, anthropometric measurements and blood sampling were collected for biochemical investigations. Thereafter the volunteers were divided into five groups: a control group (C); the other four groups received daily one of the following supplements: 200 g of deep yellow apricots (Prunus armeniaca) (AF); 250 g pomegranate (Punica granatum) juice (PJ); mixture of 100 g (PJ) and 150 g of fermented sobya (FS), (PJ-FS) or 165 g (FS). After completing the three-week dietary intervention, blood samples were collected and blood indices were again evaluated. Biochemical analysis of total lipids (TL), total glycerides (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) and tHcy were completed. The low density lipoprotein-cholesterol level and three indices of risk factors were calculated using the appropriate equations. Results: Three-week dietary intervention improved the levels of lipid profile parameters TG, TC, HDL-C and LDL-C as compared to the respective baseline levels and the response of the control group that did not receive any supplement. Consuming (PJ-FS) had a synergistic beneficially added value on some of the above mentioned lipid profile parameters. The mean plasma tHcy concentrations; a marker for occlusive vascular disease was reduced significantly following the three week consumption of (PJ) or (FS), compared with the respective pre-feeding levels. Conclusion: Pomegranate juice (PJ) or lactic acid fermented sobya (FS) or mixture of both are potential natural agents in lowering the plasma levels of lipid profile parameters and tHcy among apparently healthy young women and men, without dyslipidemic symptoms.
The ACCESS Investigators (2011) Management of Acute Coronary Syndromes in Developing Countries: Acute Coronary Events—A Multinational Survey of Current Management Strategies. American Heart Journal, 162, 852-859.
Abdel-Aziz, T. (2000) The Lipid Profile among Egyptians (LPE)—1st National Survey of Lipid Profiles and Ischaemic Heart Disease (IHD). The Egyptian Central Agency for Public Mobilization & Statistics (CAPMAS), Cairo.
Fernandez, G., Spatz, E., Jablecki, C. and Phillips, P.S. (2011) Statin Myopathy: A Common Dilemma Not Reflected in Clinical Trials. Cleveland Clinic Journal of Medicine, 78, 393-403. http://dx.doi.org/10.3949/ccjm.78a.10073
Ooi, L.-G. and Liong, M.-T. (2010) Cholesterol-Lowering Effects of Probiotics and Prebiotics: A Review of in Vivo and in Vitro Findings. International Journal of Molecular Sciences, 11, 2499-2522.
Gil, M.I., Tomas-Barberan, F.A., Hess-Pierce, B., Holcroft, D.M. and Kader, A.A. (2000) Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing. Journal of Agricultural and Food Chemistry, 48, 4581-4589. http://dx.doi.org/10.1021/jf000404a
Tucker, K.L. (2004) Dietary Intake and Coronary Heart Disease: A Variety of Nutrients and Phytochemicals Are Important. Current Treatment Options in Cardiovascular Medicine, 6, 291-302.
Esmaillzadeh, A., Tahbaz, F., Gaieni, I., Alavi-Majd, H., Azadbakht, L. (2006) Cholesterol-Lowering Effect of Concentrated Pomegranate Juice Consumption in Type II Diabetic Patients with Hyperlipidemia. International Journal for Vitamin and Nutrition Research, 76, 147-151. http://dx.doi.org/10.1024/0300-9822.214.171.124
Zhuang, G., Liu, X.M., Zhang, Q.X., Tian, F.W., Zhang, H., Zhang, H.P. and Chen, W. (2012) Research Advances with Regards to Clinical Outcome and Potential Mechanisms of the Chlestero-Lowering Effects of Probiotics. Clinical Lipidology, 7, 501-507. http://dx.doi.org/10.2217/clp.12.40
Anderson, J.W. and Gilliland, S.E. (1999) Effect of Fermented Milk (Yogurt) Containing Lactobacillus acidophilus L1 on Serum Cholesterol in Hypercholesterolemic Humans. Journal of the American College of Nutrition, 18, 43-50.
Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology, 299, 152-178.
Shireman, R.B. and Durieux, J. (1993) Microplate Methods for Determination of Serum Cholesterol, High Density Lipoprotein Cholesterol, Triglyceride and Apolipoproteins. Lipids, 28, 151-155. http://dx.doi.org/10.1007/BF02535780
Friedewald, W.T., Levy, R.I. and Fredrickson, D.S. (1972) Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, without Use of the Preparative Ultracentrifuge. Clinical Chemistry, 18, 499-502.
Casetta, B. (2010) Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS). Methods in Molecular Biology, 603, 253-260.
Lloyd-Jones, D.M., Hong, Y.L., Labarthe, D., et al. (2010) Defining and Setting National Goals for Cardiovascular Health Promotion and Disease Reduction: The American Heart Association’s Strategic Impact Goal Through 2020 and Beyond. Circulation, 121, 586-613. http://dx.doi.org/10.1161/CIRCULATIONAHA.109.192703
National Cholesterol Education Program (1994) Second Report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II). Circulation, 89, 1333-1445.
National Cholesterol Education Program (2001) Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). Journal of the American Medical Association, 285, 2486-2497.
Mertens-Talcott, S.U., Jilma-Stohlawetz, P., Rios, J., Hingorani, L. and Derendorf, H. (2006) Absorption, Metabolism, and Antioxidant Effects of Pomegranate (Punica granatum L.) Polyphenols after Ingestion of a Standardized Extract in Healthy Human Volunteers. Journal of Agricultural and Food Chemistry, 54, 8956-8961.
Tziomalos, K., Doumas, M., and Athyros, V.G. (2013) No-Pharmacological Intervention: Pomegranate Juice for the Management of Hypertension and the Improvement of Cardiovascular Health. The Open Hypertension Journal, 5, 23-26. http://dx.doi.org/10.2174/1876526201305010023
Tehranifar, A., Zarei, M., Esfandiyari, B. and Nemati, Z. (2010) Physicochemical Properties and Antioxidant Activities of Pomegranate Fruit (Punica granatum) of Different Cultivars Grown in Iran. Horticulture, Environment, and Biotechnology, 51, 573-579.
Seeram, N.P., Lee, R. and Heber, D. (2004) Bioavailability of Ellagic Acid in Human Plasma after Consumption of Ellagitannins from Pomegranate (Punica granatum L.) Juice. Clinica Chimica Acta, 348, 63-68.
Anoosh, E.G., Mojtaba, E.I. and Fatemeh, S.A. (2010) Study the Effect of Juice of Two Variety of Pomegranate on Decreasing Plasma LDL Cholesterol. Procedia-Social and Behavioral Sciences, 2, 620-623.
Asgary, S., Sahebkar, A., Afshani, M.R., Keshvari, M., Haghjooyjavanmard, S. and Rafieian-Kopaei, M. (2014) Clinical Evaluation of Blood Pressure Lowering, Endothelial Function Improving, Hypolipidemic and Anti-Inflammatory Effects of Pomegranate Juice in Hypertensive Subjects. Phytotherapy Research, 28, 193-199.
Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D. and Fuhrman, B. (2000) Pomegranate Juice Consumption Reduces Oxidative Stress, Atherogenic Modifications to LDL, and Platelet Aggregation: Studies in Humans and in Atherosclerotic Apolipoprotein E-Deficient Mice. American Journal of Clinical Nutrition, 71, 1062-1076.
Sumner, M.D., Elliott-Eller, M., Weidner, G., Daubenmier, J.J., Chew, M.H., Marlin, R., Raisin, C.J. and Ornish, D. (2005) Effects of Pomegranate Juice Consumption on Myocardial Perfusion in Patients with Coronary Heart Disease. American Journal of Cardiology, 96, 810-814. http://dx.doi.org/10.1016/j.amjcard.2005.05.026
Kelishadi, R., Gidding, S.S., Hashemi, M., Hashemipour, M., Zakerameli, A. and Poursafa, P. (2011) Acute and Long Term Effects of Grape and Pomegranate Juice Consumption on Endothelial Dysfunction in Pediatric Metabolic Syndrome. Journal of Research in Medical Sciences, 16, 245-253.
Liu, K., Xing, A., Chen, K., Wang, B., Zhou, R., Chen, S., Xu, H. and Mi, M. (2013) Effect of Fruit Juice on Cholesterol and Blood Pressure in Adults: A Meta-Analysis of 19 Randomized Controlled Trials. PloS ONE, 8, e61420.
Barkas, F. and Liberopoulos, E. (2013) Is pomegranate Juice Readily Available and Useful for the Management of Hypertension and the Improvement of Cardiovascular Health? The Open Hypertension Journal, 5, 40.
Bialonska, D., Ramnani, P., Kasimsetty, S.G., Muntha, K.R., Gibson, G.R. and Ferreira, D. (2010) The Influence of Pomegranate By-Product and Punicalagins on Selected Groups of Human Intestinal Microbiota. International Journal of Food Microbiology, 140,175-182. http://dx.doi.org/10.1016/j.ijfoodmicro.2010.03.038
Mathew, A.S, Capel-Williams, G.M., Berry, S.E. and Hall, W.L. (2012) Acute Effects of Pomegranate Extract on Postprandial Lipaemia, Vascular Function and Blood Pressure. Plant Foods for Human Nutrition, 67, 351-357.
Le Chatelier, E., Nielsen, T., Qin, J.J., Prifti, E., Hildebrand, F., et al. (2013) Richness of Human Gut Microbiome Correlates with Metabolic Markers. Nature, 500, 541-546. http://dx.doi.org/10.1038/nature12506
Lansky, E.P. and Newman, R.A. (2007) Punica granatum (Pomegranate) and Its Potential for Prevention and Treatment of Inflammation and Cancer. Journal of Ethnopharmacology, 109, 177-206.
Kumar, M., Ravinder Nagpal, R., Kumar, R., Hemalatha, R., Verma, V., Kumar, A., Chakraborty, C., Singh, B., Marotta, F., Jain, S. and Yadav, H. (2012) Cholesterol-Lowering Probiotics as Potential Biotherapeutics for Metabolic Diseases. Experimental Diabetes Research, 2012, Article ID: 902917.
Kieling, G., Schneider, J. and Jahreis, G. (2002) Long-Term Consumption of Fermented Dairy Products over 6 Months Increases HDL Cholesterol. European Journal of Clinical Nutrition, 56, 843-849.
Hussein, L., Gouda, M., Fouad, M., Labib, E., Bassyouni, R. and Mohammad, M. (2014) Dietary Intervention with Yoghurt, Synbiotic Yogurt or Traditional Fermented Sobya: Bio-Potency among Male Adolescents Using Five Bio-Markers of Relevance to Colonic Metabolic Activities. Food and Nutrition Sciences, 5, 1131-1144.
Refsum, H., Smith, A.D., Ueland, P.M., Nexo, E., Clarke, R., McPartlin, J., Johnston, C., Engbaek, F., Schneede, J., McPartlin, C. and Scott, J.M. (2004) Facts and Recommendations about Total Homocysteine Determinations: An Expert Opinion. Clinical Chemistry, 50, 3-32. http://dx.doi.org/10.1373/clinchem.2003.021634
Refsum, H., Yajnik, C.S., Gadkari, M., Schneede, J., Vollset, S.E., Orning, L., et al. (2001) Hyperhomocysteinemia and Elevated Methylmalonic Acid Indicate a High Prevalence of Cobalamin Deficiency in Asian Indians. American Journal of Clinical Nutrition, 74, 233-241.
Bhagwat, V.R., Yadav, A.S. and Rathod, I.M. (2009) Homocysteine, Lipid Indices and Antioxidants in Patients with Ischaemic Heart Disease from Maharashtra, India. Singapore Medical Journal, 50, 418-424.