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Quantitative Estimation of Gallic Acid as Biomarker in Lipitame Tablets by HPTLC Densitometry for Diabetic Dyslipidemia

DOI: 10.4236/cm.2014.54021, PP. 170-178

Keywords: Lipitame, Tablets, Quantitative Estimation, Densitometry, Diabetic Dyslipidemia

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Abstract:

Lipitame is a poly herbal formulation comprised of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica. The formulation is investigated for its analysis evaluation. Biomarkers of three herbs such as Terminalia arjuna, Terminalia belerica, and Phyllanthus emblica have been already cited to contain gallic acid, which was qualitatively and quantitatively estimated. In the present study rapid and inexpensive qualification methods for the quality control of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica on thin layer chromatography (TLC) were developed and validated. The solvent system used was toluene:ethyl acetate:formic acid:methanol (12:9:4:0.5). The scanning of plate was performed linearly at 273 nm (absorption) by use of a TLC Scanner III CAMAG with a deuterium source, and the area of spots corresponding to Gallic acid standard was integrated. It was found that gallic acid has been found in the Lipitame tablets on HPTLC densitometry assessment compared with authentic gallic acid reference standard.

References

[1]  Modi, J., Soni, H., Pandya, K., Patel, G. and Patel, N. (2014) A Detail Phyto-Chemical Evaluation of Herbo-Mineral Formulation Used in Respiratory Diseases. Journal of Pharmacognosy and Phytochemistry, 2, 36-42.
[2]  Desai, L., Oza, J. and Khatri, K. (2012) Prospective Process Validation of Polyherbal Cough Syrup Formulation. Journal of Advanced Pharmaceutical Technology & Research, 2, 225-231.
[3]  Khdair, A., Mohammad, M.K., Tawaha, K., Al-Hamarsheh, E., AlKhatib, H.S., Al-khalidi, B., Bustanji, Y., Najjar, S. and Hudaib, M. (2010) A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup. International Journal of Analytical Chemistry, 2010, Article ID: 478143, 5 p.
[4]  Herbal Formulation Comprising Extracts of Adhatoda, Hedychium and Curcuma as Cough Syrup, WIPO Patent Application (2005) WO/2005/077393 Kind Code: A1.
[5]  Saha, A., Pawar, V.M. and Jayaraman, S. (2012) Characterisation of Polyphenols in Terminalia arjuna Bark Extract. Indian Journal of Pharmaceutical Sciences, 74, 339-347.
[6]  Said, H.M. (1970) Emblica Officinalis. In: Hamdard Pharmacopoeia of Eastern Medicine, The Times Press, Karachi, 383.
[7]  Jacob, A., Pandey, M., Kapoor, S. and Saroja, R. (1988) Effect of the Indian Gooseberry (Amla) on Serum Cholesterol Levels in Men Aged 35 55 Years. European Journal of Clinical Nutrition, 42, 939-944.
[8]  Mathur, R., Sharma, A., Dixit, V.P. and Varma, M. (1996) Hypolipidaemic Effect of Fruit Juice of Emblica officinalis in Cholesterol-Fed Rabbits. Journal of Ethnopharmacology, 50, 61-68.
http://dx.doi.org/10.1016/0378-8741(95)01308-3
[9]  Kim, H.J., Yokozawa, T., Kim, H.Y., Tohda, C., Rao, T.P. and Juneja, L.R. (2005) Influence of Amla (Emblica officinalis Gaertn.) on Hypercholesterolemia and Lipid Peroxidation in Cholesterol-Fed Rats. Journal of Nutritional Science and Vitaminology, 51, 413-418.
[10]  Anila, L. and Vijayalakshmi, N.R. (2002) Flavonoids from Emblica officinalis and Mangifera indica—Effectiveness for Dyslipidemia. Journal of Ethnopharmacology, 79, 81-87.
http://dx.doi.org/10.1016/S0378-8741(01)00361-0
[11]  Rajak, S., Banerjee, S.K., Sood, S., Dinda, A.K., Gupta, Y.K., Gupta, S.K. and Maulik, S.K. (2004) Emblica officinalis Causes Myocardial Adaptation and Protects against Oxidative Stress in Ischemic-Reperfusion Injury in Rats. Phytotherapy Research, 18, 54-60.
http://dx.doi.org/10.1002/ptr.1367
[12]  Liu, L., Li, B. and Wang, L. (2005) Effect of Emblica officinalis on Formation of Atherosclerotic Plaque in Hypercholesterolemia Rabbits. Applied Journal of General Practice, 2, 97-98.
[13]  Qureshi, S.A., Asad, W. and Sultana, V. (2009) The Effect of Phyllanthus emblica Linn on Type-II Diabetes, Triglycerides and Liver-Specific Enzyme. Pakistan Journal of Nutrition, 8, 125-128.
http://dx.doi.org/10.3923/pjn.2009.125.128
[14]  Mand, J.K., Soni, J.L., Gupta, P.P. and Singh, R. (1991) Effect of Amla (Ebmlica officinalis) on the Development of Artherosclerosis in Hypocholesterolemic Rabbits. Journal of Research and Education in Indian Medicine, 10, 1-7.
[15]  Pharmacopoeia Committee (2003) Monographs on Herbal Drugs of Unani Medicine. Drugs Control and Traditional Medicine Division, National Institute of Health, Islamabad, Vol. 1, 530.
[16]  Kapoor, L.D. (1990) Ayurvedic Medicinal Plants. In: CRC Handbook of Ayurvedic Medicinal Plants, CRC Press, India, 322.
[17]  Cheng, H.Y., Lin, T.C., Yu, K.H., Yang, C.M. and Lin, C.C. (2003) Antioxidant and Free Radical Scavenging Activities of Terminalia chebula. Biological & Pharmaceutical Bulletin, 26, 1331-1335.
[18]  Chattopadhyay, R.R. and Bhattacharyya, S.K. (2007) Plant Review Terminalia chebula: An Update. Pharmacognosy Reviews, 26, 1331-1335.

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