Enzymatic activities are important to be quantified in products as enzymatic cleaners, which are used in medical and surgical devices reprocessing. Enzymatic activities are critical for the proper chemical cleaning that intends to remove solid organic dirt from inaccessible sites. The most important enzyme for this purpose is the protease, which is able to dissolve the main dirt attached to medical and surgical instruments. In this context, this study contributes to the development of a new proteolytic activity quantification method and its validation. The methodology is based on colorimetry and uses a UV-Vis spectrophotometer to measure the substrate hydrolysis by the blue color intensity, employing Protazyme AK tablets as substrate.
Ransjö, U., Engström, L., Håkansson, P., Ledel, T., Lindgren, L., Lindqvist, A.L., Marcusson, E. and Rudbäck, K. (2001) A Test for Cleaning and Disinfection Processes in a Washer-Disinfector. APMIS, 109, 299-304.
Lopes, L.S., Silva, F.S.Q., Silva, A.S. and Delgado, I.F. (2013) Validation of a UV Spectrophotometric Method for Determination of Proteolytic Activity of Enzymatic Detergents. Journal of Surfactants and Detergents, 16, 445-448.
Agência Nacional de Vigilancia Sanitária (Anvisa) (2012) RDC no 55 de 14 de novembro de 2012—Dispõe sobre os detergentes enzimáticos de uso restrito em estabelecimentos de assistência à saúde com indicação para limpeza de dispositivos médicos e dá outras providências. Diário Oficial da Uniõo, Brasília.
Valls, C., Pujadas, G., Garcia-Vallve, S. and Mulero, M. (2011) Characterization of the Protease Activity of Detergents. Biochemistry and Molecular Biology Education, 39, 280-290. http://dx.doi.org/10.1002/bmb.20488
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Galo, A.L. and Colombo, M.F. (2009) Long Optical Path Length Spectrophotometry in Conventional Double-Beam Spectrophotometers: A Simple Alternative for Investigating Samples of Very Low Optical Density. Química Nova, 32, 488-492. http://dx.doi.org/10.1590/S0100-40422009000200036
International Organization for Standardization (1994) ISO 5725-3. Accuracy (Trueness and Precision) of Measurement Methods and Results—Part 3: Intermediate Measures of the Precision of a Standard Measurement Method. ISO/TC 69 Applications of Statistical Methods. Geneva.
Polonini, H.C., Santos, F.C., Vaz, U.P., Brandão, M.A.F., Raposo, N.R.B. and Ferreira, A.O. (2011) Development and Validation of Analytical Method for Determination of Simvastatin in Capsules. Química Nova, 34, 516-519.