The Light Emitting Diode
(LED) is a phototherapy equipment, poorly studied, which is able of acting on
blood level and it has frequently appeared in clinical practice of
physiotherapy to aid on skin rejuvenation, control inflammation and wound
healing. The aim of the study was to evaluate the effects of LED on red blood
cells through the spectrophotometer. This is an experimental study with a
sample of 5 male Wistar rats weighting between 200 g and 350 g, randomly
selected, anesthetized and 4 ml of blood was collected by cardiac puncture. The
blood collected was divided into four groups, one control and three treated,
these individually irradiated by blue LED (420 - 490 nm), green LED (515 - 570
nm) and red LED (620 - 680 nm) at an output of 3 watts, for 10 minutes and 5 cm
apart from the surface of the blood, then it was analyzed by a
spectrophotometer at 540 nm. The results were submitted to Anova and Post-roch
Turkey (p< 0.05). All
hemolysis greater than the control in hypotonic solutions of NaCl (range 0.02
to 0.06 M) (p< 0.01).
Only the band between 515 - 570 nm provides a higher hemolysis (range 0.06 to
0.1) when compared to the control group (p< 0.05). The irradiation of blood
through the LED therapy was able to weaken the cell membrane of the red blood
cells. It is likely that hemolysis does not bring harm the body, because as it
is a local therapy, the amount of hemolysis that can be caused is small and it
will induce the reproduction of new red blood cells, thus improving their
Meyer, P.F., Filho, S.D.S., Bonelli, L., Fonseca, A.S., Costa, I.C.C., Ronzio, O.A., Neto, J.B., Medeiros, A.C. and Filho, M.B. (2007) Consequences of the Magnetic Field, Sonic and Radiofrequency Waves and Intense Pulsed Light on the Labeling of Blood Constituents with Technetium-99m. Brazilian Archives of Biology and Technology, 50, 117-122.
Moreira, M.C. (2009) Utilizacao de conversores eletronicos que alimentam LEDs de alto brilho na aplicacao de tecido humano e sua interacao terapêutica. Universidade Federal de Santa Maria, Doutorado em Engenharia Elétrica, Santa Maria.
Baez, F. and Reilly, L.R. (2007) The Use of Light-Emitting Diode Therapy in the Treatment of Photoaged Skin. Journal of Cosmetic Dermatology, 6, 189-194. http://dx.doi.org/10.1111/j.1473-2165.2007.00329.x
Cavalcanti, T.C., Gregorini, C.C., Guimaraes, F., Rettori, O. and Vieira-Matos, A.N. (2003) Changes in Red Blood Cell Osmotic Fragility Induced by Total Plasma and Plasma Fractions Obtained from Rats Bearing Progressive and Regressive Variants of the Walker 256 Tumor. Brazilian Journal of Medical and Biological Research, 36, 887-895.
Goldberg, D.J. and Russell, B.A. (2006) Combination Blue (415 nm) and Red (633 nm) LED Phototherapy in the Treatment of Mild to Severe Acne Vulgaris. Journal of Cosmetic and Laser Therapy, 8, 71-75.
Weiss, R.A., Macdaniel, D.H., Geronemus, R.G. and Weiss, M.A. (2005) Clinical Trial of a Novel Non-Thermal LED Array for Reversal of Photoaging: Clinical, Histologic, and Surface Profilometric Results. Lasers in Surgery and Medicine, 36, 85-91. http://dx.doi.org/10.1002/lsm.20107
Lim, W., Lee, S., Kim, S., Chung, M., Kim, M., Lim, H., Park, J., Kim, O. and Choir, H.C. (2007) The Anti-Inflammatory Mechanism of 635 nm Light-Emitting-Diode Irradiation Compared with Existing Cox Inhibitors. Lasers in Surgery and Medicine, 39, 614-621. http://dx.doi.org/10.1002/lsm.20533
Takezaki, S., Omi, T., Sato, S. and Kawana, S. (2006) Light-Emitting Diode Phototherapy at 630 +/? 3 nm Increases Local Levels of Skin-Homing t-Cells in Human Subjects. Journal of Nippon Medical School, 73, 75-81.