A new, rapid and efficient reverse phase
Liquid Chromatography (RP-LC) method was developed for determination of
Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and
vegetative tissue of Epithelantha
micromeris. Purification of GA4 was carried out by solid phase extraction
(SPE), in Epithelantha micromeris. In
the chromatography method was obtaining a retention time of 2.1 min, using
Hypersil GOLD C-18 column (100 ×4.6 mm dim and size particle 5 μ), mobile
phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out
by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The
obtained correlation coefficient was 0.995.
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