All Title Author
Keywords Abstract


Protein Bodies in Cotyledon Cells Exhibit Differential Patterns of Legumin-Like Proteins Mobilization during Seedling Germinating States

DOI: 10.4236/ajps.2013.412304, PP. 2444-2454

Keywords: 11S Globulins, Cotyledon, Endosperm, In Vitro Germination, Legumin-Like Proteins, Olea europaea L., Protein Bodies, Seed Proteins Mobilization

Full-Text   Cite this paper   Add to My Lib

Abstract:

Olive (Olea europaea L.) tree is one of the most extensive and important agricultural crop in Mediterranean countries due to its beneficial health and nutritional properties and its high economic value. Currently, olive tree constitutes the sixth most important cultivated plant in the world, spreading from the Mediterranean region of origin to new production areas such as Australia, South and North America and South Africa. However, the mobilization processes of storage materials i.e. reserve proteins during seed germination, which are largely involved in essential physiological process including plant growth and development, remain poorly understood. Morphometric and immunohistochemistry analyses of protein bodies contained in olive seed storage tissues, cotyledon and endosperm, were performed by using different microscopy techniques, including light (bright-field and fluorescence) microscopy and transmission electron microscopy. Furthermore, we used legumin-like proteins (11S-type globulins) as a molecular marker to study the mobilization of reserve proteins from PBs of cotyledons at germinating seedling stages by using immunofluorescence assays. Results demonstrated that cotyledon and endosperm are characterized by distinct PBs populations containing legumin-like proteins, distinctly discriminated by the number of PBs per cell and tissue, size, immunofluorescence and histochemical staining. These features reflect differential PBs biogenesis during development and maturation processes in olive seed tissues endosperm and cotyledon, in relation to proteins (polypeptides) final composition, SSPs processing and/or packaging during seed maturation. Three different mobilization patterns of legumin-like proteins were identified for the first time in cotyledon PBs

References

[1]  M. Otegui, R. Herder, J. Schulze, R. Jung and A. Staehelin, “The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies,” Plant Cell, Vol. 18, No. 10, 2006, pp. 2567-2581. http://dx.doi.org/10.1105/tpc.106.040931
[2]  E. M. Herman and B. A. Larkins, “Protein Storage Bodies and Vacuoles,” Plant Cell, Vol. 11, No. 4, 1999, pp. 601-614.
[3]  M. Otegui, R. Herder, J. Schulze, R. Jung and A. Staehelin, “The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies,” Plant Cell, 18, No. 10, 2006, 2567-2581.
http://dx.doi.org/10.1105/tpc.106.040931
[4]  A. D. Shutov, H. Bäulein, F. R. Blattner and R. Müntz, “Storage and Mobilization as Antagonistic Functional Constraints on Seed Storage Globulin Evolution,” Journal of Experimental Botany, Vol. 54, No. 388, 2003, pp. 1645-1654. http://dx.doi.org/10.1093/jxb/erg165
[5]  L. Jiang, T. E. Phillips, C. A. Hamm, Y. M. Drozdowicz, P. A. Rea, M. Maeshima, S. W. Rogers and J. C. Rogers, “The Protein Storage Vacuole: A Unique Compound Organelle,” Journal Of Cell Biology, Vol. 155, No. 6, 2001, pp. 991-1002. http://dx.doi.org/10.1083/jcb.200107012
[6]  H. Q. Zheng and A. Staehelin, “Protein Storage Vacuoles Are Transformed into Lytic Vacuoles in Root Meristematic Cells of Germinating Seedlings by Multiple, Cell Type-Specific Mechanisms,” Plant Physiology, Vol. 155, No. 4, 2011, pp. 629-639.
http://dx.doi.org/10.1104/pp.110.170159
[7]  H. T. Kim, U.-K. Choi, S. H. Ryu, S. J. Lee and O.-S. Kwon, “Mobilization of Storage Proteins in Soybean Seed (Glycine max L.) during Germination and Seedling Growth,” Biochimica and Biophysica Acta, Vol. 1814, No. 9, 2011, pp. 1178-1187.
http://dx.doi.org/10.1016/j.bbapap.2011.05.004
[8]  I. M. Brocard-Gifford, T. J. Lynch and R. R. Finkelstein, “Regulatory Networks in Seeds: Integrating Developmental, Abscisic Acid, Sugar, and Light Signaling,” Plant Physiology, Vol. 131, No. 1, 2003, pp. 78-92.
http://dx.doi.org/10.1104/pp.011916
[9]  A. L. Tan-Wilson and K. A. Wilson, “Mobilization of Seed Protein Reserves,” Physiologia Plantarum, Vol. 145, No. 1, 2012, Vol. 140-153.
[10]  J. D. Bewley, K. Bradford, H. Hilhorst and H. Nonogaki, “Seeds: Physiology of Development, Germination and Dormancy,” 3rd Edition, Springer, Berlin, 2013, p. 195.
[11]  H. Nonogaki, F. Chen and K. Bradford, “Mechanisms and Genes Involved in Germination Sensu Stricto,” In: K. Bradford and H. Nonogaki, Eds., Seed Development, Dormancy and Germination, Blackwell, Oxford, 2007, pp. 264-304. http://dx.doi.org/10.1002/9780470988848.ch11
[12]  J. Tiedemann, B. Neubohn and K. Muntz, “Different Functions of Vicilin and Legumin Are Reflected in the Histopattern of Globulin Mobilization during Germination of Vetch (Vicia sativa L.),” Planta, Vol. 211, No. 1, 2000, pp. 1-12. http://dx.doi.org/10.1007/s004250000259
[13]  A. Schlereth, C. Becker, C. Horstmann, J. Tiedemann and K. Muntz, “Comparison of Globulin Mobilization and Cysteine Proteinases in Embryonic Axes and Cotyledons during Germination and Seedling Growth of Vetch (Vicia sativa L.),” Journal of Experimental Botany, Vol. 51, 349, 2000, pp. 1423-1433.
http://dx.doi.org/10.1093/jexbot/51.349.1423
[14]  T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Plant Physiology, Vol. 15, No. 3, 1962, pp. 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[15]  J. A. Bergeron and M. Singer, “Metachromasy: An Experimental and Theoretical Reevaluation,” Journal of Biophysical and Biochemical Cytology, Vol. 4, No. 4, 1958, pp. 433-457. http://dx.doi.org/10.1083/jcb.4.4.433
[16]  J. Parker, “Stains for Strands in Sieve Tubes,” Stain Technology, Vol. 40, No. 4, 1965, pp. 223-225.
[17]  J. D. Alché, J. C. Jimenez-Lopez, W. Wang, A. J. Castro and M. I. Rodriguez-Garcia, “Biochemical Characterization and Cellular Localization of 11S Type Storage Proteins in Olive (Olea europaea L.) Seeds,” Journal of Agricultural and Food Chemistry, Vol. 54, No. 15, 2006, pp. 5562-5570. http://dx.doi.org/10.1021/jf060203s
[18]  J. T. Madison, J. F. Thompson and A. E. Muenster, “Turnover of Storage Protein in Seeds of Soybean and Pea,” Annals of Botany, Vol. 47, No. 1, 1981, pp. 65-73.
[19]  K. Gallardo, C. Signor, J. Vandekerckhoye, R. Thompson and J. Burstin, “Proteomics of Medigo tranculata Seed Development Establishes the Time Frame of Diverse Metabolic Processes Related to Reserve Accumulation,” Plant Physiology, Vol. 133, No. 2, 2003, pp. 664-668.
http://dx.doi.org/10.1104/pp.103.025254
[20]  B. Hoh, G. Hinz, B. K. Jeong and D. G. Robinson, “Protein Storage Vacuoles Form de Novo during Pea Cotyledon Development,” Journal of Cell Science, Vol. 108, Pt. 1, 1995, pp. 299-310.
[21]  C. Job, L. Rajjou, Y. Lovigny, M. Belghazi and D. Job, “Patterns of Protein Oxidation in Arabidopsis Seeds and during Germination,” Plant Physiology, Vol. 138, No. 2, 2005, pp. 790-802.
http://dx.doi.org/10.1104/pp.105.062778
[22]  M. Abirached-Darmency, F. Dessaint, E. Benlicha and C. Schneider, “Biogenesis of Protein Bodies during Vicilin Accumulation in Medicago truncatula Immature Seeds,” BMC Research Notes, Vol. 5, 2012, p. 409.
http://dx.doi.org/10.1186/1756-0500-5-409
[23]  L. Tian, L. L. Dai, Z. J. Yin, M. Fukuda, T. Kumamaru, X. B. Dong, X. P. Xu and L. Q. Qu, “Small GTPase Sar1 Is Crucial for Proglutelin and α-Globulin Export from the Endoplasmic Reticulum in Rice Endosperm,” Journal of Experimental Botany, Vol. 64, No. 10, 2013, pp. 2831-2845. http://dx.doi.org/10.1093/jxb/ert128
[24]  K. Muntz, “Deposition of Storage Proteins,” Plant Molecular Biology, Vol. 38, No. 1-2, 1998, pp. 77-99.
http://dx.doi.org/10.1023/A:1006020208380
[25]  A. Zienkiewicz, J. C. Jimenez-Lopez, K. Zienkiewicz, J. D. Alché and M. I. Rodríguez-García, “Development of the Cotyledon Cells during Olive (Olea europaea L.) in Vitro Seed Germination and Seedling Growth,” Protoplasma, Vol. 248, No. 4, 2011, pp. 751-765., 2011, pp. 751-765.
[26]  G. B. Fincher, “Molecular and Cellular Biology Associated with Endosperm Mobilization in Germinating Cereal Grains,” Annual Review of Physiology Plant Molecular Biology, Vol. 40, 1989, pp. 305-346.
http://dx.doi.org/10.1146/annurev.pp.40.060189.001513
[27]  K. Muntz, “Proteases and Proteolytic Cleavage of Storage Proteins in Developing and Germination Dicot Seeds,” Journal of Experimental Biology, Vol. 47, No. 298, 1996, pp. 605-622.
[28]  M. Wink, “The Plant Vacuole: A Multifunction Compartment,” Journal of Experimental Botany, Vol. 44, 1993, pp. 231-246.
[29]  Y. Oda, T. Higaki, S. Hasezawa and N. Kutsuna, “New Insights into Plant Vacuolar Structure and Dynamics,” International Review of Cell and Molecular Biology, Vol. 277, 2009, pp. 103-135.
[30]  T. Shimada, K. Yamada, M. Kataoka, S. Nakaune, Y. Koumoto, M. Kuroyanagi, S. Tabata, T. Kato, K. Shinozaki, M. Seki, M. Kobayashi, M. Kondo, M. Nishimura and I. Hara-Nishimura, “Vacuolar Processing Enzymes Are Essential for Proper Processing of Seed Storage Proteins in Arabidopsis Thaliana,” Journal of Biological Chemistry, Vol. 278, No. 34, 2003, pp. 32292-32299.
http://dx.doi.org/10.1074/jbc.M305740200
[31]  S. D. V. F. da Rosa, M. B. McDonald, A. D. Veiga, F. del Vilela and I. A. Ferreira, “Staging Coffee Seedling Growth: A Rationale for Shortening the Coffee Seed Germination Test,” Seed Science and Technology, Vol. 38, No. 2, 2010, pp. 421-431.
[32]  I. Hara and H. Matsubara, “Pumpkin (Cucurbita sp.) seed Globulin. V. Proteolytic Activities Involved in Globulin Degradation in Ungerminated Seeds,” Plant and Cell Physiology, Vol. 21, No. 2, 1980, pp. 219-232.
[33]  G. Psaras, K. Georghiou and K. Mitrakos, “Red-Light-Induced Endosperm Preparation for radicle Protrusion of Lettuce Embryos,” Botanical Gazette, Vol. 142, No. 1, 1981, pp. 13-18. http://dx.doi.org/10.1086/337190
[34]  A. Schlereth, C. Becker, C. Horstmann, J. Tiedemann and K. Muntz, “Comparison of Globulin Mobilization and Cysteine Proteinases in Embryonic Axes and Cotyledons during Germination and Seedling Growth of Vetch (Vicia sativa L.),” Journal Experimental Botany, Vol. 51, No. 349, 2000, pp. 1423-1433.
http://dx.doi.org/10.1093/jexbot/51.349.1423
[35]  J. Tiedemann, A. Schlereth and K. Müntz, “Differential Tissue-Specific Expression of Cysteine Proteinases Forms the Basis for the Fine-Tuned Mobilization of Storage Globulin during and after Germination in Legume Seeds,” Planta, Vol. 212, No. 5-6, 2001, pp. 728-738.
http://dx.doi.org/10.1007/s004250000435
[36]  K. Müntz, “Protein Dynamics and Proteolysis in Plant Vacuoles,” Journal of Experimental Botany, Vol. 58, No. 10, 2007, pp. 2391-2407.
[37]  E. L. Virgil and T. K. Fang, “Protease Activities and Elongation Growth of Excised Cotton Seed Axes during the First 24h of Imbibition,” Seed Science Research, Vol. 5, No. 4, 1995, pp. 201-207.
[38]  P. C. Bethke, S. J. Swanson, S. Hillmer and R. L. Jones, “From Storage Compartment to Lytic Organelle: The Metamorphosis of the Aleurone Protein Storage Vacuole,” Annals of Botany, Vol. 82, No. 4, 1998, pp. 399-412.
http://dx.doi.org/10.1006/anbo.1998.0702
[39]  D. Gruis, J. Schulze and R. Jung, “Storage Protein Accumulation in the Absence of the Vacuolar Processing Enzyme Family of Cysteine Proteases,” Plant Cell, Vol. 16, No. 1, 2004, pp. 270-290.
http://dx.doi.org/10.1105/tpc.016378
[40]  C. Shi and L. L. Xu, “Characters of Cysteine Endopeptidases in Wheat Endosperm during Seed Germination and Subsequent Seedling Growth,” Journal of Integrative Plant Biology, Vol. 51, No. 1, 2009, pp. 52-57.
http://dx.doi.org/10.1111/j.1744-7909.2008.00778.x
[41]  J. Fischer, C. Becker, S. Hillmer, C. Horstmann, B. Neubohn, A. Schlereth, V. Senyuk, A. Shutov and K. Müntz, “The Families of Papain- and Legumain-Like Cysteine Proteinases from Embryonic Axes and Cotyledons of Vicia Seeds. Developmental Patterns, Intracellular Localization and Functions in Globulin Proteolysis,” Plant Molecular Biology, Vol. 43, No. 1, 2000, pp. 83-101.
http://dx.doi.org/10.1023/A:1006456615373
[42]  N. Y. Zhang and B. L. Jones, “Purification and Partial Characterization of a 31-kDa Cysteine Endopeptidase from Germinated Barley,” Planta, Vol. 199, No. 4, 1996, pp. 565-572. http://dx.doi.org/10.1007/BF00195188
[43]  G. Galili and E. M. Herman, “Protein Bodies: Storage Vacuoles in Seeds,” Advances in Botanical Research, Vol. 25, 1997, pp. 113-140.
[44]  Y. J. Ahn and G. Q. Chen, “Temporal and Spatial Expression of 2S Albumin in Castor (Ricinus communis L.),” Journal of Agricultural and Food Chemistry, Vol. 55, No. 24, 2007, pp. 10043-10049.
http://dx.doi.org/10.1021/jf071272p
[45]  N. Y. Zhang and B. L. Jones, “Characterization of Germinated Barley Endoproteolytic Enzymes by Two Dimensional Gel Electrophoresis,” Journal of Cereal Science, Vol. 21, No. 2, 1995, pp. 145-153.
http://dx.doi.org/10.1016/0733-5210(95)90030-6
[46]  N. Y. Zhang and B. L. Jones, “Polymorphism of Aspartic Proteinases in Resting and Germinating Barley Seeds,” Cereal Chemistry, Vol. 76, No. 1, 1999, pp. 134-138.
http://dx.doi.org/10.1094/CCHEM.1999.76.1.134
[47]  M. J. Chrispeels and E. M. Herman, “Endoplasmic Reticulum-Derived Compartments Function in Storage and as Mediators of Vacuolar Remodeling via a New Type of Organelle, Precursor Protease Vesicles,” Plant Physiology, Vol. 123, No. 4, 2000, pp. 1227-1233.
http://dx.doi.org/10.1104/pp.123.4.1227

Full-Text

comments powered by Disqus