heterantha commonly called indigo bush is a
member of leguminoseae family found in the Himalayan region of Kashmir. A
lectin has been isolated from the seeds of Indigofera
heterantha by the purification procedure involving anion exchange chromatography
on DEAE-cellulose followed by gel filtration chromatography on Sephadex G 100.
Molecular characterization of the lectin was done by gel filtration and
SDS-PAGE. Activity of the lectin was checked by hemagglutination assay and the
sugar specificity by sugar inhibition tests. The antimicrobial activity of the
purified lectin was carried out by Agar disc diffusion using appropriate
standards. On the ion exchange column, the bound protein when eluted with 0-0.5 M NaCl gradient emerged as three peaks—peak I, peak II and peak III out of
which only peak II showed the hemagglutinating activity. The lectin further
resolved into two peaks G1 and G2 on gel filtration, with the lectin activity
residing in G1, corresponding to a molecular weight of 70 KDa. The purified
lectin named as Indigofera heterantha Lectin
(IHL) produced a single band on SDS PAGE (18 KDa), revealing the tetrameric
nature of the lectin. It agglutinated human erythrocytes (A, B, AB, and O).
Hemagglutination was inhibited by D-galactose, Dmannose and D-arabinose. The
lectin is reasonably thermostable showing full activity within a temperature
range of 30°C to 90°C. pH stability of the lectin falls in the range of 2-9.
IHL demonstrated a remarkable antibacterial activity against the pathogenic
bacteria Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. IHL also inhibited the growth
Liu, B., Li, C.Y., Bian, H.J., Min, M.W., Chen, L.F. and Bao, J.K. (2009) Antiproliferative activity and apoptosisinducing mechanism of concanavalin A on human melanoma A375 cells. Archives of Biochemistry and Biophysics, 482, 1-6. http://dx.doi.org/10.1016/j.abb.2008.12.003
Oliveira, M.D., Andrade, C.A., Santos-Magalhaes, N.S., Coelho, L.C., Teixeira, J.A., Carneiro-da-Cunha, M.G., et al. (2008) Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity. Letters in Applied Microbiology, 46, 371-376. http://dx.doi.org/10.1111/j.1472-765X.2007.02319.x
Sun, J., Wang, L., Wang, B., Guo, Z., Liu, M., Jiang, K., et al. (2008) Purification and characterization of a natural lectin from the plasma of the shrimp Fenneropenaeus chinensis. Fish and Shellfish Immunology, 25, 290-297. http://dx.doi.org/10.1016/j.fsi.2008.06.001
Bourne, Y., Ayouba, A., Rouge, P. and Cambillau, C. (1994) Interaction of a legume lectin with two components of the bacterial cell wall. A crystallographic study. The Journal of Biological Chemistry, 269, 9429-9435.
Cammue, B.P., Peeters, B. and Peumans, W.J. (1985) Isolation and partial characterization of an N-acetylgalactosamine-specific lectin from winter-aconite (Eranthis hyemalis) root tubers. The Biochemical Journal, 227, 949-955.
Ngai, P.H. and Ng, T.B. (2007) A mannose-specific tetrameric lectin with mitogenic and antibacterial activities from the ovary of a teleost, the cobia (Rachycentron canadum). Applied Microbiology and Biotechnology, 74, 433-438. http://dx.doi.org/10.1007/s00253-006-0649-2
Wang, H., Ye, X.Y. and Ng, T.B. (2001) Purification of chrysancorin, a novel antifungal protein with mitogenic activity from garland chrysanthemum seeds. Biological Chemistry, 382, 947-951. http://dx.doi.org/10.1515/BC.2001.118
Castillo-Villanueva, A., Caballero-Ortega, H., Abdullaev-Jafarova, F., Garfias, Y., del Carmen Jimenez-Martinez, M., Bouquelet, S., et al. (2007) Lectin from Phaseolus acutifolius var. escumite: Chemical characterization, sugar specificity, and effect on human T-lymphocytes. Journal of Agricultural and Food Chemistry, 55, 5781-5787. http://dx.doi.org/10.1021/jf063644k
Adenike, K.O.O., Kemi, A.M.A. and Toyin, O. (2009) Purification of a mannose/glucose-specific lectin with antifungal activity from pepper seeds (Capsicum annuum). African Journal of Biochemistry Research, 3, 272-278.
Ngai, P.H. and Ng, T.B. (2004) A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells. Biochemical and Biophysical Research Communications, 314, 988-993. http://dx.doi.org/10.1016/j.bbrc.2003.12.196
Talas-Ogras, T., Ipekci, Z., Bajrovic, K. and Gozukirmizi, N. (2005) Antibacterial activity of seed proteins of Robinia pseudoacacia. Fitoterapia, 76, 67-72. http://dx.doi.org/10.1016/j.fitote.2004.10.020
Yan, Q., Jiang, Z., Yang, S., Deng, W. and Han, L. (2005) A novel homodimeric lectin from Astragalus mongholicus with antifungal activity. Archives of Biochemistry and Biophysics, 442, 72-81. http://dx.doi.org/10.1016/j.abb.2005.07.019
Ye, X.Y., Ng, T.B., Tsang, P.W. and Wang, J. (2001) Isolation of a homodimeric lectin with antifungal and antiviral activities from red kidney bean (Phaseolus vulgaris) seeds. Journal of Protein Chemistry, 20, 367-375. http://dx.doi.org/10.1023/A:1012276619686