The patho-physiology of the gastritis achieved a significant progress in 1983, when it was proven that Helicobacter pylori colonized in the gastric mucosa. Several studies confirmed the relationship between Helicobacter pylori (H. pylori) and the type B gastritis. Further study reported the evidence of H. pylori colonization in the gastric anthrum of almost all duodenal ulcer patients. Since this micro-organism infected almost all subjects through out all ages, epidemiological data was therefore very important to determine the strategy to control the disease. This study aims to obtain the prevalence of the positivity of the antibody against. H. pylori (anti-Hp) among the volunteer blood donors in Surakarta. The passive haemagglutination (PHA) method (Biomedik-Mataram) was carried out to test the anti-Hp among 511 healthy subjects. The results obtained were 1984 (36%) showed anti-Hp positive. There is no significant difference between male and female groups in this study (p>0.05). The results of this study can be used as a preliminary study for further research to complete the epidemiological data of the H. pylori infectivity in Surakarta.