Chlorine and sulfate toxicity in water and soils are the main factors limiting growth and yield of most plants. Tolerance and sensitive indexes related to Cl and SO4 in irrigation water and effect of nitrogen fertilizer on barley were evaluated in a completely randomized design with three replications, under greenhouse conditions. Quantity salinity tolerance and susceptibility indices such as Mean Productivity (MP), Tolerance Index (TOL), Geometric Mean Productivity (GMP), Stress Susceptibility Index (SSI), Reduction Yield Ratio (Yr) and Stress Tolerance Index (STI) on the basis of plant yield with stress (Ys) and without stress (Yp) conditions were determined. Results showed that STI had a positive and highest significant correlation with grain and straw yields, compared to other indexes. Study of Standard Beta contents in grain and straw with STI index showed that the impact of Cl had a greater effect on reduction of salinity tolerance than SO4. Also Cl/SO4 ratios of 1:3 and 1:2 for grain and 1:2 and 1:1 for plant straw brought about highest tolerance to salinity, compared to non stress conditions. The scatter plot also confirmed such findings.