The purpose of the study is to evaluate the use of the pollen as bio-indicator of environmental pollution. The content in Fe, Mn, and Zn from pollen samples uniformly distributed in Dambovita region was determined. We have collected samples during April 2009 from 18 bee houses from private farms, kipped in industrial-urban and non industrial-rural areas. The samples were analyzed with an Elvax ED-XRF spectrometer having a solid state Si-pin-diode detector with thermo-electrical cooling with 165 eV at 5.9 keV (Fe55 isotope) energy resolution. We found differences between the two kinds of samples and we concluded that the pollen can be used for bio-monitoring of environment for Fe, Mn, and Zn.