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Comparative HPTLC fingerprint profile of Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms fruits

Keywords: Ilicium verum , Illicium griffithii , HPTLC fingerprinting , Absorption spectra.

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Abstract:

Objective: To establish the fingerprint profile of two Illicium species namely Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms using high performace thin layer chromatography (HPTLC) technique. Methods: CAMAG HPTLC system equipped with TLC autosampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software was used. n-hexane, chloroform and ethyl acetate extracts of the fruits were developed in suitable mobile phase using standard procedures and scanned under UV at 254nm, 366 nm and under visible light. Results: The HPTLC fingerprinting of the n-hexane, chloroform and ethyl acetate extracts showed several peaks with different Rf values. Conclusion: HPTLC fingerprint profile of two Illicium species may be useful in differentiating the species and also helpful in the identification and authentication of these species

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